Western Blot (WB)
(Western Blot: Sample: Recombinant LAMC3, Mouse.)
Rabbit anti-Mouse Laminin Gamma 3 (LAMC3) Polyclonal Antibody | anti-LAMC3 antibody
Polyclonal Antibody to Laminin Gamma 3 (LAMC3)
Gene Names
Lamc3; AI562206; AW240805
Reactivity
Mouse
Applications
Immunocytochemistry, Immunohistochemistry, ELISA, Western Blot
Purity
Affinity Chromatography
Synonyms
Laminin Gamma 3 (LAMC3); Polyclonal Antibody; Polyclonal Antibody to Laminin Gamma 3 (LAMC3); anti-LAMC3 antibody
Host
Rabbit
Reactivity
Mouse
Clonality
Polyclonal
Specificity
The antibody is a rabbit polyclonal antibody raised against LAMC3. It has been selected for its ability to recognize LAMC3 in immunohistochemical staining andwestern blotting.
Purity/Purification
Affinity Chromatography
Form/Format
Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3,50% glycerol.
Concentration
500ug/mL (varies by lot)
Sequence
Antigen: The target protein is fused with N-terminal His-Tag and its sequence is listed below.
MGHHHHHHSGSEF- GFYGNAFSGR ADDCQPCPCP GQSACATIPE SGDVVCTHCP PGQRGRRCES CEDGFFGDPL GLSGAPQPCR RCQCSGNVDL NAVGNCDPHS GHCLRCLYNT TGAHCEHCRE GFYGSAVATR PVDKC
MGHHHHHHSGSEF- GFYGNAFSGR ADDCQPCPCP GQSACATIPE SGDVVCTHCP PGQRGRRCES CEDGFFGDPL GLSGAPQPCR RCQCSGNVDL NAVGNCDPHS GHCLRCLYNT TGAHCEHCRE GFYGSAVATR PVDKC
Applicable Applications for anti-LAMC3 antibody
Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB)
Application Notes
Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Immunogen
Recombinant LAMC3 (Gly751~Cys875) expressed in E.coli.
Cross Reactivity
Mouse
Conjugated Antibody
The APC conjugated antibody version of this item is also available as catalog #MBS2062954
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Western Blot (WB)
(Western Blot: Sample: Recombinant LAMC3, Mouse.)
Western Blot (WB)
(Western Blot: Sample: Recombinant LAMC3, Mouse.)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Mouse Liver Tissue))
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Mouse Liver Tissue))
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
172,322 Da
NCBI Official Full Name
laminin subunit gamma-3
NCBI Official Synonym Full Names
laminin gamma 3
NCBI Official Symbol
Lamc3
NCBI Official Synonym Symbols
AI562206; AW240805
NCBI Protein Information
laminin subunit gamma-3
UniProt Protein Name
Laminin subunit gamma-3
Protein Family
UniProt Gene Name
Lamc3
Uniprot Description
LAMC3: Binding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components. Defects in LAMC3 are the cause of cortical malformations occipital (OCCM). OCCM is a disease in which affected individuals develop seizures, sometimes associated with transient visual changes. Brain MRI shows both pachygyria and polymicrogyria restricted to the lateral occipital lobes.
Protein type: Extracellular matrix; Motility/polarity/chemotaxis; Secreted; Secreted, signal peptide
Chromosomal Location of Human Ortholog: 2|2 B
Cellular Component: basement membrane; extracellular region; proteinaceous extracellular matrix
Biological Process: astrocyte development; cell adhesion; cellular morphogenesis during differentiation; retina development in camera-type eye; visual perception
Research Articles on LAMC3
1. Deletion of Lamb2 and Lamc3 genes affects astrocyte migration and spatial patterning in the retina.
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Product Notes
The LAMC3 lamc3 (Catalog #AAA2002440) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Laminin Gamma 3 (LAMC3) reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Laminin Gamma 3 (LAMC3) can be used in a range of immunoassay formats including, but not limited to, Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB). Western blotting: 1:100-400 Immunocytochemistry in formalin fixed cells: 1:100-500 Immunohistochemistry in formalin fixed frozen section: 1:100-500 Immunohistochemistry in paraffin section: 1:50-200 Enzyme-linked Immunosorbent Assay: 1:100-200. Researchers should empirically determine the suitability of the LAMC3 lamc3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Antigen: The target protein is fused with N-terminal His-Tag and its sequence is listed below. MGHHHHHHSG SEF- GFYGNAFSGR ADDCQPCPCP GQSACATIPE SGDVVCTHCP PGQRGRRCES CEDGFFGDPL GLSGAPQPCR RCQCSGNVDL NAVGNCDPHS GHCLRCLYNT TGAHCEHCRE GFYGSAVATR PVDKC. It is sometimes possible for the material contained within the vial of "Laminin Gamma 3 (LAMC3), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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