Rat Prostate Specific Antigen ELISA Kit | PSA elisa kit

Rat Prostate Specific Antigen ELISA Kit

Cat. Num. AAA760132

• Gene Names: KLK3; APS; PSA; hK3; KLK2A1
• Reactivity: Rat
• Synonyms: Prostate Specific Antigen; Rat Prostate Specific Antigen ELISA Kit; PSA/KLK3/APS seminin/EC 3.4.21/EC 3.4.21.77/Gamma-seminoprotein/gamma-seminoprotein/hK3/kallikrein 3; (prostate specific antigen)/Kallikrein-3/kallikrein-3/kallikrein-related peptidase 3/KLK2A1/KLK3/P-30 antigen/prostate specific antigen/PSA semenogelase/Semenogelase/Seminin; PSA elisa kit

• Reactivity: Rat
• Specificity: This assay has high sensitivity and excellent specificity for detection of PSA. No significant cross-reactivity or interference between PSA and analogues was observed.
• Sequence Length: 227

• Samples: Serum, plasma, tissue homogenates and other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 0.156-10ng/ml
• Sensitivity: 0.094ng/ml
• Intra-assay Precision: CV<8%
• Inter-assay Precision: CV<10%
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for PSA elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Capture antibody was pre-coated onto 96-well plates. And the biotin conjugated antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of target can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 24370944
• NCBI GeneID: 354
• UniProt Accession #: Q8IXI4; Q15096; Q16272; Q86TG8; C9JXH3; G3V0H4; G3XAE3; P07288
• Molecular Weight: 25,116 Da
• NCBI Official Full Name: prostate specific antigen
• NCBI Official Synonym Full Names: kallikrein related peptidase 3
• NCBI Official Symbol: KLK3
• NCBI Official Synonym Symbols: APS; PSA; hK3; KLK2A1
• NCBI Protein Information: prostate-specific antigen
• UniProt Protein Name: Prostate-specific antigen
• UniProt Gene Name: KLK3
• UniProt Synonym Gene Names: APS; PSA; Seminin
• UniProt Entry Name: KLK3_HUMAN

NCBI Description

Kallikreins are a subgroup of serine proteases having diverse physiological functions. Growing evidence suggests that many kallikreins are implicated in carcinogenesis and some have potential as novel cancer and other disease biomarkers. This gene is one of the fifteen kallikrein subfamily members located in a cluster on chromosome 19. Its protein product is a protease present in seminal plasma. It is thought to function normally in the liquefaction of seminal coagulum, presumably by hydrolysis of the high molecular mass seminal vesicle protein. Serum level of this protein, called PSA in the clinical setting, is useful in the diagnosis and monitoring of prostatic carcinoma. Alternate splicing of this gene generates several transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]

Uniprot Description

Hydrolyzes semenogelin-1 thus leading to the liquefaction of the seminal coagulum.