Goat anti-Mouse Laminin A4 Polyclonal Antibody
Laminin A4 (Biotin)
Reactivity
Mouse
Applications
Western Blot
Purity
Affinity PurifiedPurified by mouse Laminin a4 affinity chromatography and then biotinylated.
Synonyms
Laminin A4; Polyclonal Antibody; Laminin A4 (Biotin); Anti -Laminin A4 (Biotin); anti-Laminin A4 antibody
Host
Goat
Reactivity
Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
Selected for use as a detection antibody in mouse Laminin a4 Western Blots.
Purity/Purification
Affinity Purified
Purified by mouse Laminin a4 affinity chromatography and then biotinylated.
Purified by mouse Laminin a4 affinity chromatography and then biotinylated.
Form/Format
Supplied as a lyophilized powder from a 0.2um filtered solution in PBS containing 50ug of BSA per 1ug of antibody. Reconstitute with sterile TBS, pH 7.3 (20mM Trizma base, 150mM sodium chloride) containing 0.1% BSA, 40-50% glycerol. If 1ml of buffer is us
Applicable Applications for anti-Laminin A4 antibody
Western Blot (WB)
Application Notes
Western Blot: This antibody can be used at 0.1-0.2ug/ml with the appropriate secondary reagents to detect recombinant mouse Laminin 4. The detection limit for rmLaminin 4 is approximately 10 ng/lane under non-reducing and reducing conditions., Optimal dilutions should be determined by each laboratory for each application.
Immunogen
NS0-derived rmLaminin a4 (aa826-1816)
Preparation and Storage
Lyophilized powder may be stored at 4 degree C for short-term only. Reconstitute to nominal volume by adding sterile TBS with 40-50% glycerol and store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Related Product Information for anti-Laminin A4 antibody
Laminins are heterotrimeric glycoproteins and are major components of the basement membrane. Each laminin is comprised of a single,, and chain that heterotrimerize via their coiled-coil domains to form a large cruciform-shaped molecule. Mature Laminin 4 is a subunit of Laminin 411, Laminin 421, and Laminin 423. Mature Laminin 4 is a 1792 amino acid (aa) residue protein that has a Laminin N-terminal domain, four Laminin EGF-like domains, the coiled-coil domain, and five C-terminal tandem Laminin G-like domains. The G-like domains contain binding sites for integrin, heparin, and dystroglycan. Within the region used as immunogen, mouse Laminin 4 shows 91% and 97% aa sequence identity with human and rat Laminin 4, respectively.
Product Categories/Family for anti-Laminin A4 antibody
Similar Products
Product Notes
The Laminin A4 (Catalog #AAA617500) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Laminin A4 (Biotin) reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Laminin A4 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Western Blot: This antibody can be used at 0.1-0.2ug/ml with the appropriate secondary reagents to detect recombinant mouse Laminin 4. The detection limit for rmLaminin 4 is approximately 10 ng/lane under non-reducing and reducing conditions., Optimal dilutions should be determined by each laboratory for each application. Researchers should empirically determine the suitability of the Laminin A4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Laminin A4, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
