Testing Data
(Mouse section of xortex satined with MBS555738 (red). Blue is DAPI staining of DNA. This antibody reveals strong staining in the basement membranes of blood vessels.)
Rabbit anti-Mouse Laminin 111 Polyclonal Antibody
Laminin 111, Affinity Purified, Rabbit
Reactivity
Mouse
Applications
Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot
Purity
Affinity purified antibody
Synonyms
Laminin 111; Polyclonal Antibody; Affinity Purified; Rabbit; anti-Laminin 111 antibody
Host
Rabbit
Reactivity
Mouse
Clonality
Polyclonal
Purity/Purification
Affinity purified antibody
Form/Format
Liquid
Concentration
1.0 mg/mL (varies by lot)
Applicable Applications for anti-Laminin 111 antibody
Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blot (WB)
Application Notes
Immunofluorescence/Immunohistochemistry: 1:100-5000
Western Blot: 1:10000-5000
Dilutions listed as recommendation.
Optimal dilutions should be determined by investigator.
Western Blot: 1:10000-5000
Dilutions listed as recommendation.
Optimal dilutions should be determined by investigator.
Immunogen Sequence
Laminin-111 isolated from mouse EHS cells
HGNC name : LAMA1, LAMB1, LAMC1
HGNC name : LAMA1, LAMB1, LAMC1
Preparation and Storage
Antibody can also be aliquotted and stored frozen at -20°C to -70°C in a manual defrost freezer for six months without detectable loss of activity. The antibody can be stored at 2-8°C for 1 month without detectable loss of activity. Avoid repeated freeze thaw cycles.
Testing Data
(Mouse section of xortex satined with MBS555738 (red). Blue is DAPI staining of DNA. This antibody reveals strong staining in the basement membranes of blood vessels.)
Testing Data
(Mouse section of xortex satined with MBS555738 (red). Blue is DAPI staining of DNA. This antibody reveals strong staining in the basement membranes of blood vessels.)
Western Blot (WB)
(Western Blot analysis of MBS555738.Blot rat heart cell lysates (lane 1) and 0.2 ug of purified laminin-111 protein from mouse EHS sarcoma (lane 2) was probed with MBS555738. This antibody recognizes 3 laminin isotypes: a1(440 kda) beta (220kd) and y1 (220 kda).Also recognized is a laminin binding protein at 120 kda in both rat heart lysates and purified laminin protein. Since this protein always coexpresses with lamini this crossreactivity is irrelevant.)
Western Blot (WB)
(Western Blot analysis of MBS555738.Blot rat heart cell lysates (lane 1) and 0.2 ug of purified laminin-111 protein from mouse EHS sarcoma (lane 2) was probed with MBS555738. This antibody recognizes 3 laminin isotypes: a1(440 kda) beta (220kd) and y1 (220 kda).Also recognized is a laminin binding protein at 120 kda in both rat heart lysates and purified laminin protein. Since this protein always coexpresses with lamini this crossreactivity is irrelevant.)
Related Product Information for anti-Laminin 111 antibody
Laminins are high-molecular weight (~400kDa) proteins of the extracellular matrix. They are an important and biologically active part of the basal lamina, which is one of the layers of the basement membrane, a protein network foundation for most cells and organs, influencing cell differentiation, migration, and adhesion. Laminins are heterotrimeric proteins that contain an alpha-chain, a beta-chain, and a gamma-chain. The distribution of the different laminin isoforms is tissue-specific. The laminin molecules are named according to their chain composition.
Laminin-111 is one of the first laminin isoforms to be discovered. The "111" identifies the isoform's chain composition of alpha1beta1gamma1. Laminin-111 is predominantly expressed in the embryonic epithelium, but can also be found in some adult epithelium such as the kidney,liver, testis, ovaries, and brain blood vessels. Laminin-111 has been identified as a crucial molecule for development of the embryo, genetic ablation of laminin-111 results in embryonic death.
Laminin-111 is one of the first laminin isoforms to be discovered. The "111" identifies the isoform's chain composition of alpha1beta1gamma1. Laminin-111 is predominantly expressed in the embryonic epithelium, but can also be found in some adult epithelium such as the kidney,liver, testis, ovaries, and brain blood vessels. Laminin-111 has been identified as a crucial molecule for development of the embryo, genetic ablation of laminin-111 results in embryonic death.
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Product Notes
The Laminin 111 (Catalog #AAA555738) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Laminin 111, Affinity Purified, Rabbit reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Laminin 111 can be used in a range of immunoassay formats including, but not limited to, Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blot (WB). Immunofluorescence/Immunohistochemistry: 1:100-5000 Western Blot: 1:10000-5000 Dilutions listed as recommendation. Optimal dilutions should be determined by investigator. Researchers should empirically determine the suitability of the Laminin 111 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Laminin 111, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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