Testing Data
(Anti-Laminin Picoband antibody, MBS177438-2.jpgAll lanes: Anti Laminin (MBS177438) at 0.5ug/mlLane 1: HELA Whole Cell Lysate at 40ugLane 2: HEPG2 Whole Cell Lysate at 40ugPredicted bind size: 177KDObserved bind size: 177KD )
Rabbit anti-Human Laminin subunit gamma-1&Laminin subunit gamma-2&Laminin subunit gamma-3 Polyclonal Antibody | anti-LAMC1&LAMC2&LAMC3 antibody
Anti-Laminin Antibody
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Immunohistochemistry (Paraffin-embedded Section): Concentration: 0.5-1mug/ml; Tested Species: Human; Antigen Retrieval: By heat
Immunocytochemistry: Concentration: 0.5-1ug/ml; Tested Species: Human
WB: The detection limitg for laminin is approximately 0.25ng/lane under reducing conditions.
By heat: Boiling the paraffin section in 10mM citrate buffer, pH6.0, for 20mins is required for the stainng of formalin/paraffin sections.
Other applications have not been tested.
Optimal dilutions should be determined by end users.
Testing Data
(Anti-Laminin Picoband antibody, MBS177438-2.jpgAll lanes: Anti Laminin (MBS177438) at 0.5ug/mlLane 1: HELA Whole Cell Lysate at 40ugLane 2: HEPG2 Whole Cell Lysate at 40ugPredicted bind size: 177KDObserved bind size: 177KD )
Testing Data
(Anti-Laminin Picoband antibody, MBS177438-2.jpgAll lanes: Anti Laminin (MBS177438) at 0.5ug/mlLane 1: HELA Whole Cell Lysate at 40ugLane 2: HEPG2 Whole Cell Lysate at 40ugPredicted bind size: 177KDObserved bind size: 177KD )
Immunohistochemistry (IHC)
(Anti-Laminin Picoband antibody, MBS177438-3.JPGIHC(P): Human Lung Cancer Tissue )
Immunohistochemistry (IHC)
(Anti-Laminin Picoband antibody, MBS177438-3.JPGIHC(P): Human Lung Cancer Tissue )
Background: Laminins are major proteins in the basal lamina (one of the layers of the basement membrane), a protein network foundation for most cells and organs. Laminins form independent networks and are associated with type IV collagen networks via entactin, fibronectin, and perlecan. They are important and biologically active parts of the basal lamina, influencing cell differentiation, migration, and adhesion, as well as phenotype and survival. Laminins are trimeric proteins that contain an alpha-chain, a beta-chain, and a gamma-chain, found in five, four, and three genetic variants, respectively. Laminins critically contribute to cell attachment and differentiation, cell shape and movement, maintenance of tissue phenotype, and promotion of tissue survival.
NCBI and Uniprot Product Information
NCBI Description
Laminins, a family of extracellular matrix glycoproteins, are the major noncollagenous constituent of basement membranes. They have been implicated in a wide variety of biological processes including cell adhesion, differentiation, migration, signaling, neurite outgrowth and metastasis. Laminins, composed of 3 non identical chains: laminin alpha, beta and gamma (formerly A, B1, and B2, respectively), have a cruciform structure consisting of 3 short arms, each formed by a different chain, and a long arm composed of all 3 chains. Each laminin chain is a multidomain protein encoded by a distinct gene. Several isoforms of each chain have been described. Different alpha, beta and gamma chain isomers combine to give rise to different heterotrimeric laminin isoforms which are designated by Arabic numerals in the order of their discovery, i.e. alpha1beta1gamma1 heterotrimer is laminin 1. The biological functions of the different chains and trimer molecules are largely unknown, but some of the chains have been shown to differ with respect to their tissue distribution, presumably reflecting diverse functions in vivo. This gene encodes the gamma chain isoform laminin, gamma 1. The gamma 1 chain, formerly thought to be a beta chain, contains structural domains similar to beta chains, however, lacks the short alpha region separating domains I and II. The structural organization of this gene also suggested that it had diverged considerably from the beta chain genes. Embryos of transgenic mice in which both alleles of the gamma 1 chain gene were inactivated by homologous recombination, lacked basement membranes, indicating that laminin, gamma 1 chain is necessary for laminin heterotrimer assembly. It has been inferred by analogy with the strikingly similar 3' UTR sequence in mouse laminin gamma 1 cDNA, that multiple polyadenylation sites are utilized in human to generate the 2 different sized mRNAs (5.5 and 7.5 kb) seen on Northern analysis. [provided by RefSeq, Aug 2011]
Uniprot Description
LAMC1: Binding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components.
Protein type: Secreted, signal peptide; Motility/polarity/chemotaxis; Secreted
Chromosomal Location of Human Ortholog: 1q31
Cellular Component: extracellular matrix; extracellular space; laminin-1 complex; laminin-10 complex; extracellular region; basement membrane
Molecular Function: extracellular matrix structural constituent; glycosphingolipid binding
Biological Process: extracellular matrix disassembly; axon guidance; cell migration; hemidesmosome assembly; extracellular matrix organization and biogenesis; protein complex assembly; cell adhesion; positive regulation of epithelial cell proliferation; endoderm development
Disease: Pelvic Organ Prolapse, Susceptibility To
Research Articles on LAMC1&LAMC2&LAMC3
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Product Notes
The LAMC1&LAMC2&LAMC3 lamc1 (Catalog #AAA177438) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-Laminin Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Laminin subunit gamma-1&Laminin subunit gamma-2&Laminin subunit gamma-3 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC-P). Western Blot: Concentration: 0.1-0.5mug/ml; Tested Species: Human Immunohistochemistry (Paraffin-embedded Section): Concentration: 0.5-1mug/ml; Tested Species: Human; Antigen Retrieval: By heat Immunocytochemistry: Concentration: 0.5-1ug/ml; Tested Species: Human WB: The detection limitg for laminin is approximately 0.25ng/lane under reducing conditions.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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