Rabbit anti-Human Lamin B1 Polyclonal Antibody | anti-LMB1 antibody

Lamin B1, CT (Lamin-B1, LMB1, LMN, LMN2, LMNB, LMNB1, ADLD, MGC111419) (MaxLight 405)

Cat. Num. AAA6484651

• Gene Names: LMNB1; LMN; ADLD; LMN2; LMNB
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Ammonium Sulfate Precipitation.
• Synonyms: Lamin B1; Polyclonal Antibody; CT (Lamin-B1; LMB1; LMN; LMN2; LMNB; LMNB1; ADLD; MGC111419) (MaxLight 405); anti-LMB1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human Lamin B1.
• Purity/Purification: Purified by Ammonium Sulfate Precipitation.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight405.

• Applicable Applications for anti-LMB1 antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:50-1:100; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the C-terminal region of human Lamin B1 (KLH).
• Conjugate: MaxLight405
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight405 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis of Lamin B1 antibody (C-term) in Ramos cell line lysates (35ug/lane). Lamin B1 (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(Lamin B1 Antibody (C-term) IHC analysis in formalin fixed and paraffin embedded mouse brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the Lamin B1 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Product Categories/Family for anti-LMB1 antibody

Antibodies; Lamin; Laminin

References

1. Martin, C., J. Cell. Sci. 122(PT 10), 1551-1562 (2009). 2. Brussino, A., J. Neurol. Neurosurg. Psychiatr. 80(2), 237-240 (2009).

NCBI and Uniprot Product Information

• NCBI GI #: 5031877
• NCBI GeneID: 4001
• NCBI Accession #: NP_005564
• NCBI GenBank Nucleotide #: NM_005573
• UniProt Accession #: P20700
• Molecular Weight: 66kDa
• NCBI Official Full Name: lamin-B1 isoform 1
• NCBI Official Synonym Full Names: lamin B1
• NCBI Official Symbol: LMNB1
• NCBI Official Synonym Symbols: LMN; ADLD; LMN2; LMNB
• NCBI Protein Information: lamin-B1
• UniProt Protein Name: Lamin-B1
• UniProt Gene Name: LMNB1
• UniProt Synonym Gene Names: LMN2; LMNB
• UniProt Entry Name: LMNB1_HUMAN

NCBI Description

This gene encodes one of the two B-type lamin proteins and is a component of the nuclear lamina. A duplication of this gene is associated with autosomal dominant adult-onset leukodystrophy (ADLD). Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2015]

Uniprot Description

Lamin B1: nuclear lamins are intermediate filament proteins that constitute the lattice-like matrix at the inner face of the nuclear membrane that underlies the nuclear envelop. The lamins, highly conserved throughout evolution, are encoded by three genes in the human: LMNA, LMNB1, and LMNB2. The A-type lamins (lamin A/C) are developmentally regulated and are generally expressed in differentiated cells. The anchoring of chromatin to the nuclear lamina is involved in the control of gene expression and in DNA replication and repair. During mitosis, the nuclear lamina is reversibly disassembled as the lamin proteins are phosphorylated. B-type lamins are phosphorylated within minutes of engaging the IgM surface receptor of resting splenic B cells. Nuclear lamins are cleaved by caspases during apoptosis.

Protein type: Cytoskeletal

Chromosomal Location of Human Ortholog: 5q23.2

Cellular Component: nucleoplasm; nuclear membrane; membrane; lamin filament; nuclear inner membrane; nuclear envelope

Molecular Function: phospholipase binding; structural molecule activity

Biological Process: apoptosis; cell structure disassembly during apoptosis

Disease: Leukodystrophy, Demyelinating, Adult-onset, Autosomal Dominant