Western Blot (WB)
(L3MBTL2 antibody (pAb) tested by Immunoprecipitation. 10 ul of L3MBTL2 antibody was used to immunoprecipitate L3MBTL2 from 500 ug of HeLa nuclear cell extract (lane 2). 10 ul of rabbit IgG was used as a negative control (lane 1). The immunoprecipitated protein was detected by Western blotting using the L3MBTL2 antibody at a dilution of 1:2,000.)
Rabbit anti-Human L3MBTL2 Polyclonal Antibody | anti-L3MBTL2 antibody
L3MBTL2 antibody (pAb)
IP: 10ul per IP
WB: 1:500-1:2,000 dilution
Western Blot (WB)
(L3MBTL2 antibody (pAb) tested by Immunoprecipitation. 10 ul of L3MBTL2 antibody was used to immunoprecipitate L3MBTL2 from 500 ug of HeLa nuclear cell extract (lane 2). 10 ul of rabbit IgG was used as a negative control (lane 1). The immunoprecipitated protein was detected by Western blotting using the L3MBTL2 antibody at a dilution of 1:2,000.)
Western Blot (WB)
(L3MBTL2 antibody (pAb) tested by Immunoprecipitation. 10 ul of L3MBTL2 antibody was used to immunoprecipitate L3MBTL2 from 500 ug of HeLa nuclear cell extract (lane 2). 10 ul of rabbit IgG was used as a negative control (lane 1). The immunoprecipitated protein was detected by Western blotting using the L3MBTL2 antibody at a dilution of 1:2,000.)
Western Blot (WB)
(L3MBTL2 pAb tested by Western blot. Detection of L3MBTL2 by Western blot analysis with 20 ug HeLa nuclear extract and a 1:2,000 dilution of L3MBTL2 pAb.)
Western Blot (WB)
(L3MBTL2 pAb tested by Western blot. Detection of L3MBTL2 by Western blot analysis with 20 ug HeLa nuclear extract and a 1:2,000 dilution of L3MBTL2 pAb.)
Background: L3MBTL2 (Lethal (3) malignant brain tumor-like 2 protein) is a putative member of the polycomb group family and is the human homolog of Drosophila Sfmbt, critical for Hox gene silencing. L3MBTL2 contains one FCS-type zinc finger and four MBT (malignant brain tumor) repeats. L3MBTL2 associates with chromatin-remodeling complexes and helps inhibit the expression of proteins that trigger the cells into mitosis. L3MBTL2 is a part of a complex involved in the G0 phase of the cell cycle. This complex contains other proteins such as E2F-6, Max or HP1 gamma, and participates in transcriptional repression.
NCBI and Uniprot Product Information
Uniprot Description
Function: Putative Polycomb group (PcG) protein. PcG proteins maintain the transcriptionally repressive state of genes, probably via a modification of chromatin, rendering it heritably changed in its expressibility. Its association with a chromatin-remodeling complex suggests that it may contribute to prevent expression of genes that trigger the cell into mitosis. Binds to monomethylated and dimethylated 'Lys-20' on histone H4. Binds histone H3 peptides that are monomethylated or dimethylated on 'Lys-4', 'Lys-9' or 'Lys-27'. Ref.12
Subunit structure: Part of the E2F6.com-1 complex in G0 phase composed of E2F6, MGA, MAX, TFDP1, CBX3, BAT8, EUHMTASE1, RING1, RNF2, MBLR, BAT8 and YAF2.
Subcellular location: Nucleus
Probable.
Sequence similarities: Contains 1 FCS-type zinc finger.Contains 4 MBT repeats.
Sequence caution: The sequence BAB84917.1 differs from that shown. Reason: Intron retention.The sequence BAC04936.1 differs from that shown. Reason: Intron retention.
Research Articles on L3MBTL2
Similar Products
Product Notes
The L3MBTL2 l3mbtl2 (Catalog #AAA388494) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The L3MBTL2 antibody (pAb) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's L3MBTL2 can be used in a range of immunoassay formats including, but not limited to, Immunoprecipitation (IP), Western Blot (WB). Applications Validated: IP: 10ul per IP WB: 1:500-1:2,000 dilution. Researchers should empirically determine the suitability of the L3MBTL2 l3mbtl2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "L3MBTL2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
