Rabbit Ku-70 Polyclonal Antibody | anti-Ku-70 antibody

Ku-70 (R586) Polyclonal Antibody

Cat. Num. AAA3001189

• Gene Names: XRCC6; ML8; KU70; TLAA; CTC75; CTCBF; G22P1
• Reactivity: Human, Mouse, Rat
• Applications: Immunohistochemistry
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: Ku-70; Polyclonal Antibody; Ku-70 (R586) Polyclonal Antibody; X-ray repair cross-complementing protein 6; 5'-deoxyribose-5-phosphate lyase Ku70; 5'-dRP lyase Ku70; 70 kDa subunit of Ku antigen; ATP-dependent DNA helicase 2 subunit 1; ATP-dependent DNA helicase II 70 kDa subunit; CTC box-binding factor 75 kDa subunit; CTC75; CTCBF; DNA repair protein XRCC6; Lupus Ku autoantigen protein p70; Ku70; Thyroid-lupus autoantigen; TLAA; X-ray repair complementing defective repair in; Chinese hamster cells 6; XRCC6; G22P1; anti-Ku-70 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Ku-70 (R586) polyclonal antibody detects endogenous levels of Ku-70 protein
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 609

• Applicable Applications for anti-Ku-70 antibody: Immunohistochemistry (IHC)
• Application Notes: IHC: 1:50-1:200
• Immunogen: Synthetic peptide, corresponding to amino acids 560-600 of Human Ku-70.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Immunohistochemistry (IHC)

(Immunohistochemistry (IHC) analyzes of Ku-70 (R586) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.)

Related Product Information for anti-Ku-70 antibody

The Ku protein is localized in the nucleus and is composed of subunits referred to as Ku-70 (or p70) and Ku-86 (or p86), which is also known by the synonym Ku-80 (or p80). Ku was first described as an autoantigen to which antibodies were produced in a patient with scleroderma-polymyositis overlap syndrome, and was later found in the sera of patients with other rheumatic diseases. Both subunits of the Ku protein have been cloned, and a number of functions have been proposed for Ku, including cell signaling, DNA replication and transcriptional activation.

NCBI and Uniprot Product Information

• NCBI GI #: 4503841
• NCBI GeneID: 2547
• NCBI Accession #: NP_001460.1
• NCBI GenBank Nucleotide #: NP_001460.1
• UniProt Accession #: P12956
• Molecular Weight: ~ 70kDa
• NCBI Official Full Name: X-ray repair cross-complementing protein 6 isoform 1
• NCBI Official Synonym Full Names: X-ray repair cross complementing 6
• NCBI Official Symbol: XRCC6
• NCBI Official Synonym Symbols: ML8; KU70; TLAA; CTC75; CTCBF; G22P1
• NCBI Protein Information: X-ray repair cross-complementing protein 6
• UniProt Protein Name: X-ray repair cross-complementing protein 6
• UniProt Gene Name: XRCC6
• UniProt Synonym Gene Names: G22P1; 5'-dRP lyase Ku70; CTC75; CTCBF; Ku70; TLAA
• UniProt Entry Name: XRCC6_HUMAN

NCBI Description

The p70/p80 autoantigen is a nuclear complex consisting of two subunits with molecular masses of approximately 70 and 80 kDa. The complex functions as a single-stranded DNA-dependent ATP-dependent helicase. The complex may be involved in the repair of nonhomologous DNA ends such as that required for double-strand break repair, transposition, and V(D)J recombination. High levels of autoantibodies to p70 and p80 have been found in some patients with systemic lupus erythematosus. [provided by RefSeq, Jul 2008]

Uniprot Description

Ku70: a mini-chromosome maintenance protein, essential for the initiation of eukaryotic genome replication. Allows DNA to undergo a single round of replication per cell cycle. Required for the entry in S phase and for cell division.

Protein type: Helicase; EC 3.6.4.-; DNA-binding; DNA repair, damage; Nuclear receptor co-regulator; EC 4.2.99.-; RNA-binding

Chromosomal Location of Human Ortholog: 22q13.2

Cellular Component: nucleoplasm; transcription factor complex; nuclear telomere cap complex; membrane; nucleolus; cytosol; nucleus

Molecular Function: ATP-dependent DNA helicase activity; protein C-terminus binding; protein binding; DNA binding; double-stranded DNA binding; damaged DNA binding; double-stranded telomeric DNA binding; 5'-deoxyribose-5-phosphate lyase activity; ATP binding

Biological Process: positive regulation of neurogenesis; V(D)J recombination; transcription, DNA-dependent; viral reproduction; positive regulation of transcription, DNA-dependent; DNA repair; double-strand break repair via nonhomologous end joining; DNA duplex unwinding; DNA ligation; double-strand break repair; positive regulation of interferon type I production; innate immune response; positive regulation of transcription from RNA polymerase II promoter; brain development; negative regulation of transcription, DNA-dependent; telomere maintenance