Rabbit KSR1 Polyclonal Antibody | anti-KSR1 antibody

KSR1 Antibody

Cat. Num. AAA9613349

• Gene Names: KSR1; KSR; RSU2
• Reactivity: Human, Mouse, Rat; Predicted Reactivity: Pig (100%), Bovine (100%), Rabbit (100%), Dog (100%), Chicken (92%)
• Applications: Western Blot, ELISA
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin
• Synonyms: KSR1; Polyclonal Antibody; KSR1 Antibody; BKSR 1; BKSR1; dKsr; EK 31; EK31; hb; Kinase suppressor of Ras 1; Kinase suppressor of ras; KSR 1; KSR; KSR1_HUMAN; Positive Ras signaling mediator family member (86.4KD); Positive Ras signaling mediator family member; RSU 2; RSU2; SR 31; SR31; Suppressor of activated let 60 Ras SUR3; Suppressor of Ras1 31; anti-KSR1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat; Predicted Reactivity: Pig (100%), Bovine (100%), Rabbit (100%), Dog (100%), Chicken (92%)
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: KSR1 Antibody detects endogenous levels of total KSR1.
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin
• Form/Format: Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)

• Applicable Applications for anti-KSR1 antibody: Western Blot (WB), Peptide ELISA (EIA)
• Application Notes: WB: 1:500-1:2000; Peptide ELISA: 1:20,000-1:40,000
• Immunogen: A synthesized peptide derived from human KSR1, corresponding to a region within the internal amino acids.
• Conjugation: Unconjugated
• Fragment: Fab fragment
• Post Translational Modifications: Phosphorylated on Ser-311 and, to a higher extent, on Ser-406 by MARK3. Dephosphorylated on Ser-406 by PPP2CA. In resting cells, phosphorylated KSR1 is cytoplasmic and in stimulated cells, dephosphorylated KSR1 is membrane-associated. Phosphorylated by PKA at Ser-888. Phosphorylation at Ser-888 is required for cAMP-dependent activation of MAPK1 and/or MAPK3 (By similarity).
• Subunit Structure: Homodimer. Heterodimerizes (via N-terminus) with BRAF (via N-terminus) in a MAP2K1/MEK1 or MAP2K2/MEK2-dependent manner. Interacts with MAP2K1/MEK1 and MAP2K2/MEK2. Binding to MAP2K1/MEK1 releases the intramolecular inhibitory interaction between KSR1 N-terminus and kinase domains which is required for the subsequent RSK1 dimerization with BRAF. Identified in a complex with AKAP13, MAP2K1 and BRAF (By similarity). Interacts with AKAP13 and BRAF. Interacts with RAF and MAPK/ERK, in a Ras-dependent manner (By similarity). Interacts with 14-3-3 proteins including YWHAB (By similarity). Interacts with HSP90AA1/HSP90, YWHAE/14-3-3 and CDC37. The binding of 14-3-3 proteins to phosphorylated KSR1 prevents the membrane localization (By similarity). Interacts with MARK3 (By similarity). Interacts with PPP2R1A and PPP2CA (By similarity). Interacts with isoform 1 of VRK2.
• Similarity: The protein kinase domain is predicted to be catalytically inactive. The domain is sufficient for KSR1 and KSR1-mediated MAP2K1 and MAP2K2 membrane localization. The domain is required but not sufficient for MAP kinase-mediated inhibition of ELK1 phosphorylation (PubMed:10409742).The N-terminal region mediates interaction with BRAF (PubMed:29433126). Also mediates membrane localization (By similarity).Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family.
• Subcellular Location: Cytoplasm. Membrane>Peripheral membrane protein. Cell membrane>Peripheral membrane protein. Cell projection>Ruffle membrane. Endoplasmic reticulum membrane.; Note: In unstimulated cells, where the phosphorylated form is bound to a 14-3-3 protein, sequestration in the cytoplasm occurs. Following growth factor treatment, the protein is free for membrane translocation, and it moves from the cytoplasm to the cell periphery.
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Western Blot (WB)

(Western blot analysis of KSR1 in lysates of HepG2, using KSR1 Antibody(AF4641).)

Related Product Information for anti-KSR1 antibody

Part of a multiprotein signaling complex which promotes phosphorylation of Raf family members and activation of downstream MAP kinases (By similarity). Independently of its kinase activity, acts as MAP2K1/MEK1 and MAP2K2/MEK2-dependent allosteric activator of BRAF; upon binding to MAP2K1/MEK1 or MAP2K2/MEK2, dimerizes with BRAF and promotes BRAF-mediated phosphorylation of MAP2K1/MEK1 and/or MAP2K2/MEK2. Promotes activation of MAPK1 and/or MAPK3, both in response to EGF and to cAMP (By similarity). Its kinase activity is unsure (By similarity). Some protein kinase activity has been detected in vitro, however the physiological relevance of this activity is unknown (By similarity).

NCBI and Uniprot Product Information

• NCBI GI #: 75677325
• NCBI GeneID: 8844
• NCBI Accession #: NP_055053.1
• NCBI GenBank Nucleotide #: NM_014238.1
• UniProt Accession #: Q8IVT5; Q13476
• Molecular Weight: 102,032 Da
• NCBI Official Full Name: kinase suppressor of Ras 1
• NCBI Official Synonym Full Names: kinase suppressor of ras 1
• NCBI Official Symbol: KSR1
• NCBI Official Synonym Symbols: KSR; RSU2
• NCBI Protein Information: kinase suppressor of Ras 1
• UniProt Protein Name: Kinase suppressor of Ras 1
• Protein Family: Kinase suppressor of Ras
• UniProt Gene Name: KSR1
• UniProt Synonym Gene Names: KSR
• UniProt Entry Name: KSR1_HUMAN

Uniprot Description

KSR: a serine/threonine kinase of the Raf family with no demonstrated kinase activity. A location-regulated scaffolding protein connecting MEK to RAF. Promotes MEK and RAF phosphorylation and activity through assembly of an activated signaling complex. Interacts with RAF and MAPK/ERK, in a Ras-dependent manner. The binding of 14-3-3 proteins to phosphorylated KSR prevents the membrane localization. In unstimulated cells, the phosphorylated form is bound to a 14-3-3 protein and sequestered in the cytoplasm. Translocates from the cytoplasm to the cell periphery following TNF treatment. When Bad is available, ceramide signaling through the Ras/Raf cascade can lead to apoptosis. Three alternatively-spliced isoforms have been described.

Protein type: Protein kinase, TKL; Protein kinase, Ser/Thr (non-receptor); Kinase, protein; TKL group; RAF family

Chromosomal Location of Human Ortholog: 17q11.2

Cellular Component: endoplasmic reticulum membrane; membrane; endoplasmic reticulum

Molecular Function: protein serine/threonine kinase activity; MAP-kinase scaffold activity; protein binding; mitogen-activated protein kinase kinase binding; metal ion binding; ATP binding; protein kinase activity

Biological Process: positive regulation of MAPKKK cascade; Ras protein signal transduction; protein amino acid phosphorylation