Rabbit KSR1 Polyclonal Antibody | anti-KSR1 antibody

KSR1 Antibody

Cat. Num. AAA9611205

• Gene Names: KSR1; KSR; RSU2
• Reactivity: Human, Mouse; Predicted: Pig, Bovine, Rabbit, Dog
• Applications: Western Blot, ELISA
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Synonyms: KSR1; Polyclonal Antibody; KSR1 Antibody; BKSR 1; BKSR1; dKsr; EK 31; EK31; hb; Kinase suppressor of Ras 1; Kinase suppressor of ras; KSR 1; KSR; KSR1_HUMAN; Positive Ras signaling mediator family member (86.4 kD); Positive Ras signaling mediator family member; RSU 2; RSU2; SR 31; SR31; Suppressor of activated let 60 Ras SUR3; Suppressor of Ras1 31; anti-KSR1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse; Predicted: Pig, Bovine, Rabbit, Dog
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: KSR1 Antibody detects endogenous levels of KSR1.
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Form/Format: Liquid; Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)
• Sequence Length: 762

• Applicable Applications for anti-KSR1 antibody: Western Blot (WB), Peptide ELISA (EIA)
• Application Notes: WB: 1:500-1:2000; ELISA (Peptide): 1:20000-1:40000
• Immunogen: A synthesized peptide derived from human KSR1.
• Subcellular Location: Endoplasmic Reticulum; Plasma Membrane
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Western Blot (WB)

(Western blot analysis of KSR1 in lysates of HepG2, using KSR1 Antibody.)

Related Product Information for anti-KSR1 antibody

Description: Scaffolding protein that is part of a multiprotein signaling complex. Promotes phosphorylation of Raf family members and activation of downstream MAP kinases. Promotes activation of MAPK1 and/or MAPK3, both in response to EGF and to cAMP. Does not have kinase activity by itself.

Post Translational Modifications: Phosphorylated on Ser-311 and, to a higher extent, on Ser-406 by MARK3. Dephosphorylated on Ser-406 by PPP2CA. In resting cells, phosphorylated KSR1 is cytoplasmic and in stimulated cells, dephosphorylated KSR1 is membrane-associated. Phosphorylated by PKA at Ser-888. Phosphorylation at Ser-888 is required for cAMP-dependent activation of MAPK1 and/or MAPK3 (By similarity).

Subunit Structure: Interacts with HSP90AA1/HSP90, YWHAE/14-3-3, CDC37, MAP2K1/MEK-1 and MAP2K2/MEK-2 (PubMed:10409742). Interacts with MARK3, PPP2R1A and PPP2CA. Also interacts with RAF and MAPK/ERK, in a Ras-dependent manner. The binding of 14-3-3 proteins to phosphorylated KSR prevents the membrane localization (By similarity). Interacts with isoform 1 of VRK2 (PubMed:20679487). Interacts with AKAP13 and BRAF (PubMed:21102438). Identified in a complex with AKAP13, MAP2K1 and BRAF (By similarity).

Similarity: The protein kinase domain is predicted to be catalytically inactive. The domain is sufficient for KSR1 and KSR1-mediated MAP2K1 and MAP2K2 membrane localization. The domain is required but not sufficient for MAP kinase-mediated inhibition of ELK1 phosphorylation (PubMed:10409742). The protein kinase domain is predicted to be catalytically inactive. The domain is sufficient for KSR1 and KSR1-mediated MAP2K1 and MAP2K2 membrane localization. The domain is required but not sufficient for MAP kinase-mediated inhibition of ELK1 phosphorylation. The N-terminal region mediates interaction with BRAF and with membranes. Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family.

NCBI and Uniprot Product Information

• NCBI GI #: 75677325
• NCBI GeneID: 8844
• NCBI Accession #: NP_055053.1
• NCBI GenBank Nucleotide #: NP_055053.1
• UniProt Accession #: Q8IVT5
• Molecular Weight: Observed: 120 kDa; Predicted: 103 kDa
• NCBI Official Full Name: kinase suppressor of Ras 1 isoform 1
• NCBI Official Synonym Full Names: kinase suppressor of ras 1
• NCBI Official Symbol: KSR1
• NCBI Official Synonym Symbols: KSR; RSU2
• NCBI Protein Information: kinase suppressor of Ras 1
• UniProt Protein Name: Kinase suppressor of Ras 1
• Protein Family: Kinase suppressor of Ras
• UniProt Gene Name: KSR1
• UniProt Synonym Gene Names: KSR
• UniProt Entry Name: KSR1_HUMAN

Uniprot Description

KSR: a serine/threonine kinase of the Raf family with no demonstrated kinase activity. A location-regulated scaffolding protein connecting MEK to RAF. Promotes MEK and RAF phosphorylation and activity through assembly of an activated signaling complex. Interacts with RAF and MAPK/ERK, in a Ras-dependent manner. The binding of 14-3-3 proteins to phosphorylated KSR prevents the membrane localization. In unstimulated cells, the phosphorylated form is bound to a 14-3-3 protein and sequestered in the cytoplasm. Translocates from the cytoplasm to the cell periphery following TNF treatment. When Bad is available, ceramide signaling through the Ras/Raf cascade can lead to apoptosis. Three alternatively-spliced isoforms have been described.

Protein type: Protein kinase, TKL; Protein kinase, Ser/Thr (non-receptor); Kinase, protein; TKL group; RAF family

Chromosomal Location of Human Ortholog: 17q11.2

Cellular Component: endoplasmic reticulum membrane; membrane; endoplasmic reticulum

Molecular Function: protein serine/threonine kinase activity; MAP-kinase scaffold activity; protein binding; mitogen-activated protein kinase kinase binding; metal ion binding; ATP binding; protein kinase activity

Biological Process: positive regulation of MAPKKK cascade; Ras protein signal transduction; protein amino acid phosphorylation