Western Blot (WB)
(Western Blot analysis in mouse heart tissue lysates (35ug/lane) using MBS6011112. This demonstrates that MBS6011112 detected the KRT82 protein (arrow).)
Rabbit anti-Human, Mouse KRT82 Polyclonal Antibody | anti-Krt82 antibody
KRT82, ID (KRT82, KRTHB2, Keratin, type II cuticular Hb2, Keratin-82, Type II hair keratin Hb2, Type-II keratin Kb22)
Gene Names
Krt82; Krt2-20
Reactivity
Human, Mouse
Applications
ELISA, Western Blot, Immunohistochemistry
Purity
Affinity PurifiedPurified by Protein A affinity chromatography.
Synonyms
KRT82; Polyclonal Antibody; ID (KRT82; KRTHB2; Keratin; type II cuticular Hb2; Keratin-82; Type II hair keratin Hb2; Type-II keratin Kb22); Anti -KRT82; anti-Krt82 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
Human, mouse
Purity/Purification
Affinity Purified
Purified by Protein A affinity chromatography.
Purified by Protein A affinity chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Applicable Applications for anti-Krt82 antibody
ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
Suitable for use in Western Blot, Immunohistochemistry, ELISA
Dilution: ELISA: 1:1,000
Western Blot: 1:100-500
Immunohistochemistry: 1:50-100
Dilution: ELISA: 1:1,000
Western Blot: 1:100-500
Immunohistochemistry: 1:50-100
Immunogen
KRT82 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 320-350 amino acids from the Central region of human KRT82.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Western Blot (WB)
(Western Blot analysis in mouse heart tissue lysates (35ug/lane) using MBS6011112. This demonstrates that MBS6011112 detected the KRT82 protein (arrow).)
Western Blot (WB)
(Western Blot analysis in mouse heart tissue lysates (35ug/lane) using MBS6011112. This demonstrates that MBS6011112 detected the KRT82 protein (arrow).)
Immunohistochemistry (IHC)
(Immunohistochemistry analysis of formalin fixed and paraffin embedded human skin carcinoma using MBS6011112 followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of MBS6011112 for immunohistochemistry.)
Immunohistochemistry (IHC)
(Immunohistochemistry analysis of formalin fixed and paraffin embedded human skin carcinoma using MBS6011112 followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of MBS6011112 for immunohistochemistry.)
Related Product Information for anti-Krt82 antibody
KRT82 is a member of the keratin gene family. As a type II hair keratin, it is a basic protein which heterodimerizes with type I keratins to form hair and nails. The type II hair keratins are clustered in a region of chromosome 12q13 and are grouped into two distinct subfamilies based on structure similarity. One subfamily, consisting of KRTHB1, KRTHB3, and KRTHB6, is highly related. The other less-related subfamily includes KRTHB2, KRTHB4, and KRTHB5. All hair keratins are expressed in the hair follicle; this keratin appears to be a hair cuticle-specific keratin.
Product Categories/Family for anti-Krt82 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
57,140 Da
NCBI Official Full Name
keratin, type II cuticular Hb2
NCBI Official Synonym Full Names
keratin 82
NCBI Official Symbol
Krt82
NCBI Official Synonym Symbols
Krt2-20
NCBI Protein Information
keratin, type II cuticular Hb2; K82; keratin-82; type-II keratin Kb22; type II hair keratin Hb2; keratin complex 2, basic, gene 20
UniProt Protein Name
Keratin, type II cuticular Hb2
Protein Family
UniProt Gene Name
Krt82
UniProt Synonym Gene Names
Krt2-20; Krthb2; K82
UniProt Entry Name
KRT82_MOUSE
Uniprot Description
K82: a type II cytoskeletal keratin. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. There are two types of cytoskeletal and microfibrillar keratin: type I (acidic; 40-55 kDa) [K9 to K20] and type II (neutral to basic; 56-70 kDa) [K1 to K8]. Both a basic and an acidic keratin are required for filament assembly.
Protein type: Cytoskeletal; Motility/polarity/chemotaxis
Cellular Component: keratin filament; intermediate filament
Molecular Function: structural molecule activity
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Product Notes
The Krt82 krt82 (Catalog #AAA6011112) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The KRT82, ID (KRT82, KRTHB2, Keratin, type II cuticular Hb2, Keratin-82, Type II hair keratin Hb2, Type-II keratin Kb22) reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's KRT82 can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC). Suitable for use in Western Blot, Immunohistochemistry, ELISA Dilution: ELISA: 1:1,000 Western Blot: 1:100-500 Immunohistochemistry: 1:50-100. Researchers should empirically determine the suitability of the Krt82 krt82 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "KRT82, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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