HLA-DOB recombinant protein

HLA-DOB, 27-224aa, Human, His tag, E Coli (Denatured)

Cat. Num. AAA205672

• Gene Names: HLA-DOB; DOB; HLA_DOB
• Applications: SDS-Page
• Purity: > 95% by SDS-PAGE
• Synonyms: HLA-DOB; 27-224aa; Human; His tag; E Coli (Denatured); Major histocompatibility complex class II DO beta; Major histocompatibility complex; class II; DO beta; DOB; HLA-DOB recombinant protein

• Host: E Coli
• Purity/Purification: > 95% by SDS-PAGE
• Form/Format: Liquid. In 20mM Tris-HCl Buffer (pH 8.0) containing 0.4M urea, 10% glycerol
• Concentration: 1 mg/ml (determined by Bradford assay) (varies by lot)
• Sequence: MGSSHHHHHH SSGLVPRGSH MGSGTDSPED FVIQAKADCY FTNGTEKVQF VVRFIFNLEE YVRFDSDVGM FVALTKLGQP DAEQWNSRLD LLERSRQAVD GVCRHNYRLG APFTVGRKVQ PEVTVYPERT PLLHQHNLLH CSVTGFYPGD IKIKWFLNGQ EERAGVMSTG PIRNGDWTFQ TVVMLEMTPE LGHVYTCLVD HSSLLSPVSV EWRAQSEYSW RK
• Sequence Length: 273

• Applicable Applications for HLA-DOB recombinant protein: SDS-PAGE
• Antigen Species: Human
• Tag Information: His-tag
• Preparation and Storage: Can be stored at 4 degree C short term (1-2 weeks).; For long term storage, aliquot and store at -20 degree C or -70 degree C.; Avoid repeated freezing and thawing cycles.

SDS-Page

Related Product Information for HLA-DOB recombinant protein

HLA-DOB belongs to the HLA class II beta chain paralogues. This class II molecule is a heterodimer consisting of an alpha (DOA) and a beta chain (DOB), both anchored in the membrane. It is located in intracellular vesicles. DO suppresses peptide loading of MHC class II molecules by inhibiting HLA-DM. Class II molecules are expressed in antigen presenting cells (APC: B lymphocytes, dendritic cells, macrophages). The beta chain is approximately 26-28 kDa and its gene contains 6 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the two extracellular domains, exon 4 encodes the transmembrane domain and exon 5 encodes the cytoplasmic tail. Recombinant human HLA-DOB protein, fused to His-tag at N-terminus, was expressed in E Coli.

Product Categories/Family for HLA-DOB recombinant protein

Immunology

References

Naruse T.K., et al. (2002) Tissue Antigens. 59:512-519; Beck S., et al. (1996) J. Mol. Biol. 255:1-13;

NCBI and Uniprot Product Information

• NCBI GI #: 4504403
• NCBI GeneID: 3112
• NCBI Accession #: NP_002111
• NCBI GenBank Nucleotide #: NM_002120.3
• UniProt Accession #: P13765; Q29746; Q29825; Q6FHC2; B0V0Y0
• Molecular Weight: 25.2 kDa (222aa)
• NCBI Official Full Name: HLA class II histocompatibility antigen, DO beta chain
• NCBI Official Synonym Full Names: major histocompatibility complex, class II, DO beta
• NCBI Official Symbol: HLA-DOB
• NCBI Official Synonym Symbols: DOB; HLA_DOB
• NCBI Protein Information: HLA class II histocompatibility antigen, DO beta chain
• UniProt Protein Name: HLA class II histocompatibility antigen, DO beta chain
• Protein Family: HLA class II histocompatibility antigen
• UniProt Gene Name: HLA-DOB

NCBI Description

HLA-DOB belongs to the HLA class II beta chain paralogues. This class II molecule is a heterodimer consisting of an alpha (DOA) and a beta chain (DOB), both anchored in the membrane. It is located in intracellular vesicles. DO suppresses peptide loading of MHC class II molecules by inhibiting HLA-DM. Class II molecules are expressed in antigen presenting cells (APC: B lymphocytes, dendritic cells, macrophages). The beta chain is approximately 26-28 kDa and its gene contains 6 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the two extracellular domains, exon 4 encodes the transmembrane domain and exon 5 encodes the cytoplasmic tail. [provided by RefSeq, Jul 2008]

Uniprot Description

Important modulator in the HLA class II restricted antigen presentation pathway by interaction with the HLA-DM molecule in B-cells. Modifies peptide exchange activity of HLA-DM.