Western Blot (WB)
(KRT6C Antibody (N-term) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the KRT6C antibody detected the KRT6C protein (arrow).)
Rabbit anti-Human KRT6C Polyclonal Antibody | anti-KRT6C antibody
KRT6C, NT (KRT6C, KRT6E, Keratin, type II cytoskeletal 6C, Cytokeratin-6C, Cytokeratin-6E, Keratin K6h, Keratin-6C, Type-II keratin Kb12) (Biotin)
Gene Names
KRT6C; K6E; KRT6E; PPKNEFD
Reactivity
Human
Applications
Immunohistochemistry, ELISA, Western Blot
Purity
Purified by Protein A Affinity Chromatography.
Synonyms
KRT6C; Polyclonal Antibody; NT (KRT6C; KRT6E; Keratin; type II cytoskeletal 6C; Cytokeratin-6C; Cytokeratin-6E; Keratin K6h; Keratin-6C; Type-II keratin Kb12) (Biotin); Type-II keratin Kb12; anti-KRT6C antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Purified by Protein A Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.
Applicable Applications for anti-KRT6C antibody
Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
Application Notes
IHC: 1:10-50
ELISA: 1:1,000
WB: 1:100-500
Applications are based on unconjugated antibody.
ELISA: 1:1,000
WB: 1:100-500
Applications are based on unconjugated antibody.
Immunogen
KRT6C antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 134-164 amino acids from the N-terminal region of human KRT6C.
Conjugate
Biotin
Note
Preservative Free
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Western Blot (WB)
(KRT6C Antibody (N-term) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the KRT6C antibody detected the KRT6C protein (arrow).)
Western Blot (WB)
(KRT6C Antibody (N-term) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the KRT6C antibody detected the KRT6C protein (arrow).)
Immunohistochemistry (IHC)
(KRT6C Antibody (N-term) (AP14194a)immunohistochemistry analysis in formalin fixed and paraffin embedded human esophagus carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of KRT6C Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Immunohistochemistry (IHC)
(KRT6C Antibody (N-term) (AP14194a)immunohistochemistry analysis in formalin fixed and paraffin embedded human esophagus carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of KRT6C Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Related Product Information for anti-KRT6C antibody
Keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into epithelial keratins and hair keratins. The type II keratins are clustered in a region of chromosome 12q13. [provided by RefSeq].
Product Categories/Family for anti-KRT6C antibody
References
Bowden, P.E. J. Invest. Dermatol. 130(2):336-338(2010). Wilson, N.J., et al. J. Invest. Dermatol. 130(2):425-429(2010). Schweizer, J., et al. J. Cell Biol. 174(2):169-174(2006). Scherer, S.E., et al. Nature 440(7082):346-351(2006). Hesse, M., et al. J. Cell. Sci. 114 (PT 14), 2569-2575 (2001).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
60 kDa
NCBI Official Full Name
keratin, type II cytoskeletal 6C
NCBI Official Synonym Full Names
keratin 6C, type II
NCBI Official Symbol
KRT6C
NCBI Official Synonym Symbols
K6E; KRT6E; PPKNEFD
NCBI Protein Information
keratin, type II cytoskeletal 6C; CK-6C; CK-6E; K6C; cytokeratin-6C; cytokeratin-6E; keratin 6E; keratin K6h; type-II keratin Kb12
UniProt Protein Name
Keratin, type II cytoskeletal 6C
Protein Family
UniProt Gene Name
KRT6C
UniProt Synonym Gene Names
KRT6E; CK-6C; CK-6E; K6C
UniProt Entry Name
K2C6C_HUMAN
Uniprot Description
Subunit structure: Heterodimer of a type I and a type II keratin. KRT6 isomers associate with KRT16 and/or KRT17.
Tissue specificity: Constitutively expressed in distinct types of epithelia such as those in oral mucosa, esophagus, papillae of tongue and hair follicle outer root sheath.
Miscellaneous: There are at least six isoforms of human type II keratin-6 (K6).There are two types of cytoskeletal and microfibrillar keratin, I (acidic) and II (neutral to basic) (40-55 and 56-70 kDa, respectively).
Sequence similarities: Belongs to the intermediate filament family.
Similar Products
Product Notes
The KRT6C krt6c (Catalog #AAA6314735) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The KRT6C, NT (KRT6C, KRT6E, Keratin, type II cytoskeletal 6C, Cytokeratin-6C, Cytokeratin-6E, Keratin K6h, Keratin-6C, Type-II keratin Kb12) (Biotin) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's KRT6C can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB). IHC: 1:10-50 ELISA: 1:1,000 WB: 1:100-500 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the KRT6C krt6c for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "KRT6C, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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