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Western Blot (WB) (Western blot analysis of different mouse tissue lysates using antibody 211699.)

Rabbit KRT5 Polyclonal Antibody | anti-KRT5 antibody

KRT5 (Cytokeratin 5, Keratin 5, K5, CK5, Keratin Type II Cytoskeletal 5, Type-II Keratin Kb5, 58 kDa Cytokeratin) (ML550)

Gene Names
KRT5; K5; CK5; DDD; EBS2; KRT5A
Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Immunofluorescence, Western Blot
Purity
Purified by affinity chromatography.
Synonyms
KRT5; Polyclonal Antibody; KRT5 (Cytokeratin 5; Keratin 5; K5; CK5; Keratin Type II Cytoskeletal 5; Type-II Keratin Kb5; 58 kDa Cytokeratin) (ML550); anti-KRT5 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes human KRT5. Species Crossreactivity: mouse and rat
Purity/Purification
Purified by affinity chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight550.
Applicable Applications for anti-KRT5 antibody
Immunohistochemistry (IHC) Paraffin, Immunofluorescence (IF), Western Blot (WB)
Application Notes
IHC-P: 1:50-1:200
IF: 1:50-1:200
WB: 1:500-1:2000
Applications are based on unconjugated antibody.
Immunogen
Recombinant protein corresponding to human KRT5.
Conjugate
MaxLight550
Note
Preservative Free
Preparation and Storage
Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight550 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis of different mouse tissue lysates using antibody 211699.)

Western Blot (WB) (Western blot analysis of different mouse tissue lysates using antibody 211699.)

Immunohistochemistry (IHC)

(IHC analysis of paraffin-embedded human kidney cancer using antibody 211699.)

Immunohistochemistry (IHC) (IHC analysis of paraffin-embedded human kidney cancer using antibody 211699.)

Immunohistochemistry (IHC)

(IHC analysis of paraffin-embedded human metrocarcinoma using antibody 211699.)

Immunohistochemistry (IHC) (IHC analysis of paraffin-embedded human metrocarcinoma using antibody 211699.)
Product Categories/Family for anti-KRT5 antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
62,378 Da
NCBI Official Full Name
keratin, type II cytoskeletal 5
NCBI Official Synonym Full Names
keratin 5
NCBI Official Symbol
KRT5
NCBI Official Synonym Symbols
K5; CK5; DDD; EBS2; KRT5A
NCBI Protein Information
keratin, type II cytoskeletal 5; CK-5; cytokeratin-5; 58 kda cytokeratin; type-II keratin Kb5; epidermolysis bullosa simplex 2 Dowling-Meara/Kobner/Weber-Cockayne types; keratin 5 (epidermolysis bullosa simplex, Dowling-Meara/Kobner/Weber-Cockayne types)
UniProt Protein Name
Keratin, type II cytoskeletal 5
Protein Family
UniProt Gene Name
KRT5
UniProt Synonym Gene Names
CK-5; K5
UniProt Entry Name
K2C5_HUMAN

NCBI Description

The protein encoded by this gene is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. This type II cytokeratin is specifically expressed in the basal layer of the epidermis with family member KRT14. Mutations in these genes have been associated with a complex of diseases termed epidermolysis bullosa simplex. The type II cytokeratins are clustered in a region of chromosome 12q12-q13. [provided by RefSeq, Jul 2008]

Uniprot Description

K5: Heterotetramer of two type I and two type II keratins. Keratin-5 associates with keratin-14. Interacts with TCHP. Belongs to the intermediate filament family.

Protein type: Cytoskeletal

Chromosomal Location of Human Ortholog: 12q13.13

Cellular Component: membrane; mitochondrion; keratin filament; cytoplasm; plasma membrane; intermediate filament; cytosol; nucleus

Molecular Function: protein binding; structural constituent of cytoskeleton

Biological Process: hemidesmosome assembly; epidermis development

Disease: Epidermolysis Bullosa Simplex, Localized; Epidermolysis Bullosa Simplex, Generalized; Dowling-degos Disease 1; Epidermolysis Bullosa Simplex With Migratory Circinate Erythema; Epidermolysis Bullosa Simplex, Autosomal Recessive 1; Epidermolysis Bullosa Simplex With Mottled Pigmentation; Epidermolysis Bullosa Simplex, Dowling-meara Type

Research Articles on KRT5

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Product Notes

The KRT5 krt5 (Catalog #AAA6424270) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The KRT5 (Cytokeratin 5, Keratin 5, K5, CK5, Keratin Type II Cytoskeletal 5, Type-II Keratin Kb5, 58 kDa Cytokeratin) (ML550) reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's KRT5 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC) Paraffin, Immunofluorescence (IF), Western Blot (WB). IHC-P: 1:50-1:200 IF: 1:50-1:200 WB: 1:500-1:2000 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the KRT5 krt5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "KRT5, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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