Rat ApoC3 ELISA Kit | ApoC3 elisa kit

Rat ApoC3 (Apolipoprotein C3) ELISA Kit

Cat. Num. AAA2516294

• Gene Names: APOC3; HALP2; APOCIII
• Reactivity: Rat
• Synonyms: ApoC3; Rat ApoC3 (Apolipoprotein C3) ELISA Kit; ApoC3 elisa kit

• Reactivity: Rat
• Specificity: This kit recognizes natural and recombinant Rat ApoC3. No significant cross-reactivity or interference between Rat ApoC3 and analogues was observed.
• Sequence Length: 99

• Samples: Serum, Plasma, Biological Fluids
• Assay Type: Sandwich
• Detection Range: 6.25-400ng/ml
• Sensitivity: Min: 3.75ng/ml; Max: 400ng/ml
• Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Rat ApoC3 concentrations in serum, plasma and other biological fluids.
• Preparation and Storage: Store at 4 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for ApoC3 elisa kit

Principle of the assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to ApoC3. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for ApoC3 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain ApoC3, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of ApoC3. You can calculate the concentration of ApoC3 in the samples by comparing the OD of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 4557323
• NCBI GeneID: 345
• NCBI Accession #: NP_000031.1
• NCBI GenBank Nucleotide #: NM_000040.1
• Molecular Weight: 10,852 Da
• NCBI Official Full Name: apolipoprotein C-III
• NCBI Official Synonym Full Names: apolipoprotein C-III
• NCBI Official Symbol: APOC3
• NCBI Official Synonym Symbols: HALP2; APOCIII
• NCBI Protein Information: apolipoprotein C-III; apo-CIII; apoC-III; apolipoprotein C3
• UniProt Protein Name: Apolipoprotein C-III
• Protein Family: Apolipoprotein
• UniProt Gene Name: APOC3
• UniProt Synonym Gene Names: Apo-CIII; ApoC-III
• UniProt Entry Name: APOC3_HUMAN

NCBI Description

Apolipoprotein C-III is a very low density lipoprotein (VLDL) protein. APOC3 inhibits lipoprotein lipase and hepatic lipase; it is thought to delay catabolism of triglyceride-rich particles. The APOA1, APOC3 and APOA4 genes are closely linked in both rat and human genomes. The A-I and A-IV genes are transcribed from the same strand, while the A-1 and C-III genes are convergently transcribed. An increase in apoC-III levels induces the development of hypertriglyceridemia. [provided by RefSeq, Jul 2008]

Uniprot Description

APOC3: Inhibits lipoprotein lipase and hepatic lipase and decreases the uptake of lymph chylomicrons by hepatic cells. This suggests that it delays the catabolism of triglyceride-rich particles. Defects in APOC3 are the cause of hyperalphalipoproteinemia type 2 (HALP2). HALP2 is a condition characterized by high levels of high density lipoprotein (HDL) and increased HDL cholesterol levels. Belongs to the apolipoprotein C3 family.

Protein type: Secreted, signal peptide; Secreted; Lipid-binding

Chromosomal Location of Human Ortholog: 11q23.3

Cellular Component: extracellular space; chylomicron; early endosome; extracellular region

Molecular Function: lipase inhibitor activity; cholesterol binding; phospholipid binding; enzyme regulator activity

Biological Process: response to drug; cholesterol metabolic process; response to peptide hormone stimulus; phototransduction, visible light; regulation of Cdc42 protein signal transduction; cholesterol efflux; lipoprotein metabolic process; G-protein coupled receptor protein signaling pathway; cholesterol homeostasis; reverse cholesterol transport; triacylglycerol metabolic process; negative regulation of lipid catabolic process; phospholipid efflux; lipoprotein transport; triacylglycerol catabolic process; negative regulation of receptor-mediated endocytosis; negative regulation of lipoprotein lipase activity; triacylglycerol mobilization; retinoid metabolic process; inflammatory response; negative regulation of lipid metabolic process; negative regulation of fatty acid biosynthetic process; response to nutrient

Disease: Apolipoprotein C-iii Deficiency