Rabbit anti-Human KREMEN2 Polyclonal Antibody | anti-KREMEN2 antibody

KREMEN2, ID (KREMEN2, KRM2, Kremen protein 2, Dickkopf receptor 2, Kringle domain-containing transmembrane protein 2, Kringle-containing protein marking the eye and the nose) (Biotin)

Cat. Num. AAA6314493

• Gene Names: KREMEN2; KRM2
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: KREMEN2; Polyclonal Antibody; ID (KREMEN2; KRM2; Kremen protein 2; Dickkopf receptor 2; Kringle domain-containing transmembrane protein 2; Kringle-containing protein marking the eye and the nose) (Biotin); Kringle-containing protein marking the eye and the nose; anti-KREMEN2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human KREMEN2.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

• Applicable Applications for anti-KREMEN2 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 248-276 in the central region of human KREMEN2, UniProt Accession #Q8NCW0; NP_757384.1; NP_078783.1.
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot analysis in ZR-75-1, K562, NCI-H460 cell line lysates (35ug/lane) usingMBS641795. This demonstrates that MBS641795 detected the KREMEN2 protein (arrow).)

Related Product Information for anti-KREMEN2 antibody

This gene encodes a high-affinity dickkopf homolog 1 (DKK1) transmembrane receptor that functionally cooperates with DKK1 to block wingless (WNT)/beta-catenin signaling. The encoded protein forms a ternary membrane complex with DKK1 and the WNT receptor lipoprotein receptor-related protein 6 (LRP6), and induces rapid endocytosis and removal of LRP6 from the plasma membrane. It contains extracellular kringle, WSC, and CUB domains. Alternatively spliced transcript variants encoding distinct isoforms have been observed for this gene.

Product Categories/Family for anti-KREMEN2 antibody

Antibodies; Dickkopf Proteins

References

1. Mao, B., et al. Nature 417(6889):664-667(2002).

NCBI and Uniprot Product Information

• NCBI GI #: 27437008
• NCBI GeneID: 79412
• NCBI Accession #: NP_757384.1; NP_078783.1
• NCBI GenBank Nucleotide #: NM_172229.2
• UniProt Accession #: Q8NCW0; Q8N2J4; Q8NCW1; Q96GL8; Q9BTP9; B4DXF6; I3L2S2
• Molecular Weight: 40,433 Da
• NCBI Official Full Name: kremen protein 2 isoform d
• NCBI Official Synonym Full Names: kringle containing transmembrane protein 2
• NCBI Official Symbol: KREMEN2
• NCBI Official Synonym Symbols: KRM2
• NCBI Protein Information: kremen protein 2
• UniProt Protein Name: Kremen protein 2
• Protein Family: Kremen protein
• UniProt Gene Name: KREMEN2
• UniProt Synonym Gene Names: KRM2
• UniProt Entry Name: KREM2_HUMAN

NCBI Description

This gene encodes a high-affinity dickkopf homolog 1 (DKK1) transmembrane receptor. A similar protein in mouse functions interacts with with DKK1 to block wingless (WNT)/beta-catenin signaling. The encoded protein forms a ternary membrane complex with DKK1 and the WNT receptor lipoprotein receptor-related protein 6 (LRP6), and induces rapid endocytosis and removal of LRP6 from the plasma membrane. It contains extracellular kringle, WSC, and CUB domains. Alternatively spliced transcript variants encoding distinct isoforms have been observed for this gene. [provided by RefSeq, Dec 2011]

Uniprot Description

KREMEN2: Receptor for Dickkopf protein. Cooperates with Dickkopf to block Wnt/beta-catenin signaling. Forms a ternary complex with Dkk1 and LRP6 and induces rapid endocytosis and removal of the Wnt receptor LRP6 from the plasma membrane. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Receptor, misc.; Membrane protein, integral

Chromosomal Location of Human Ortholog: 16p13.3

Cellular Component: membrane; early endosome membrane; plasma membrane; integral to membrane

Biological Process: Wnt receptor signaling pathway; cell communication