Rabbit anti-Human KPTN Polyclonal Antibody | anti-KPTN antibody

KPTN, ID (KPTN, Kaptin, Actin-associated protein 2E4) (APC)

Cat. Num. AAA6314437

• Gene Names: KPTN; 2E4; MRT41
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: KPTN; Polyclonal Antibody; ID (KPTN; Kaptin; Actin-associated protein 2E4) (APC); Actin-associated protein 2E4; anti-KPTN antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).

• Applicable Applications for anti-KPTN antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: KPTN antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 244-273 amino acids from the Central region of human KPTN.
• Conjugate: APC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(KPTN Antibody (Center) western blot analysis in NCI-H460 cell line lysates (35ug/lane).This demonstrates the KPTN antibody detected the KPTN protein (arrow).)

Related Product Information for anti-KPTN antibody

KPTN may be involved in actin dynamics. May play a role in producing the sensory apparatus in hair cells. May play a role in actin rearrangements that accompany platelet activation and stereocilia formation.

Product Categories/Family for anti-KPTN antibody

Antibodies; Actin Related Proteins

References

Ewing, R.M., et al. Mol. Syst. Biol. 3, 89 (2007): Bearer, E.L., et al. Ann. Hum. Genet. 64 (PT 3), 189-196 (2000): Bearer, E.L., et al. Eur. J. Cell Biol. 78(2):117-126(1999). Bearer, E.L. J. Neurosci. 12(3):750-761(1992).

NCBI and Uniprot Product Information

• NCBI GI #: 103471989
• NCBI GeneID: 11133
• NCBI Accession #: NP_008990.2
• NCBI GenBank Nucleotide #: NM_007059.3
• UniProt Accession #: Q9Y664; Q96GT1; B3KN86; B4DQ76
• Molecular Weight: 21,583 Da
• NCBI Official Full Name: KICSTOR complex protein kaptin isoform 1
• NCBI Official Synonym Full Names: kaptin, actin binding protein
• NCBI Official Symbol: KPTN
• NCBI Official Synonym Symbols: 2E4; MRT41
• NCBI Protein Information: KICSTOR complex protein kaptin
• UniProt Protein Name: KICSTOR complex protein kaptin
• Protein Family: Kaptin
• UniProt Gene Name: KPTN

NCBI Description

This gene encodes a filamentous-actin-associated protein, which is involved in actin dynamics and plays an important role in neuromorphogenesis. This protein is part of the KICSTOR protein complex that localizes to lysosomes. Mutations in this gene result in an autosomal recessive form of intellectual disability. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jul 2017]

Uniprot Description

As part of the KICSTOR complex functions in the amino acid-sensing branch of the TORC1 signaling pathway. Recruits, in an amino acid-independent manner, the GATOR1 complex to the lysosomal membranes and allows its interaction with GATOR2 and the RAG GTPases. Functions upstream of the RAG GTPases and is required to negatively regulate mTORC1 signaling in absence of amino acids. In absence of the KICSTOR complex mTORC1 is constitutively localized to the lysosome and activated. The KICSTOR complex is also probably involved in the regulation of mTORC1 by glucose.