Human Alpha-methylacyl-CoA racemase ELISA Kit | AMACR elisa kit

Human Alpha-methylacyl-CoA racemase ELISA Kit

Cat. Num. AAA764693

• Gene Names: Amacr; Da1-8; Marc1
• Reactivity: Human
• Synonyms: Alpha-methylacyl-CoA racemase; Human Alpha-methylacyl-CoA racemase ELISA Kit; AMACR/AMACRD/CBAS4/RACE/RM/2-methylacyl-CoA racemase/alpha-methylacyl-CoA racemase/; AMACR elisa kit

• Reactivity: Human
• Sequence Length: 382

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 0.156-10ng/ml
• Sensitivity: 0.094ng/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for AMACR elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- AMACR antibody was pre-coated onto 96-well plates. And the biotin conjugated anti- AMACR antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the AMACR amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of AMACR can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 401709963
• NCBI GeneID: 25284
• NCBI Accession #: NP_036948.2
• NCBI GenBank Nucleotide #: NM_012816.2
• Molecular Weight: 41,828 Da
• NCBI Official Full Name: alpha-methylacyl-CoA racemase
• NCBI Official Synonym Full Names: alpha-methylacyl-CoA racemase
• NCBI Official Symbol: Amacr
• NCBI Official Synonym Symbols: Da1-8; Marc1
• NCBI Protein Information: alpha-methylacyl-CoA racemase
• UniProt Protein Name: Alpha-methylacyl-CoA racemase
• Protein Family: Alpha-methylacyl-CoA racemase
• UniProt Gene Name: Amacr
• UniProt Entry Name: AMACR_RAT

NCBI Description

enzmyme that converts pristanoyl-CoA and C27-bile acyl-CoAs to (S)-stereoisomers; involved in the degradation of the side chain of bile acid intermediates [RGD, Feb 2006]

Uniprot Description

AMACR: Racemization of 2-methyl-branched fatty acid CoA esters. Responsible for the conversion of pristanoyl-CoA and C27-bile acyl-CoAs to their (S)-stereoisomers. Has a role in drug metabolism as well as probably in lipid metabolism. The 2-arylpropionic acid (2-APA's) derivatives, including ibuprofen, are the most widely used anti-inflammatory analgesic cyclooxygenase inhibitors. The (-)-R-enantiomer, which is inactive in terms of cyclooxygenase inhibition, is epimerized in vivo via this enzyme to the cyclooxygenase-inhibiting (+)-S-enantiomer

Protein type: EC 5.1.99.4; Isomerase; Lipid Metabolism - primary bile acid biosynthesis; Mitochondrial

Cellular Component: cytoplasm; mitochondrion; peroxisome

Molecular Function: alpha-methylacyl-CoA racemase activity; catalytic activity; receptor binding

Biological Process: bile acid biosynthetic process; bile acid metabolic process; fatty acid metabolic process