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Western Blot (WB) (Western blot analysis of KNP-I expression in SW480 (A), HepG2 (B), mouse skin (C), mouse heart (D), rat skeletal muscle (E) whole cell lysates.)

Rabbit anti-Human KNP-I Polyclonal Antibody | anti-C21orf33 antibody

Anti-KNP-I Antibody

Gene Names
C21orf33; ES1; HES1; KNPH; KNPI; GT335
Reactivity
Human
Applications
Western Blot, Immunofluorescence, Immunocytochemistry
Purity
The antibody was purified by immunogen affinity chromatography.
Synonyms
KNP-I; Polyclonal Antibody; Anti-KNP-I Antibody; HES1; KNPI; ES1 protein homolog; mitochondrial; Protein GT335; Protein KNP-I; anti-C21orf33 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Specificity
Recognizes endogenous levels of KNP-I protein.
Purity/Purification
The antibody was purified by immunogen affinity chromatography.
Form/Format
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Sequence Length
268
Applicable Applications for anti-C21orf33 antibody
Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC)
Application Notes
WB: 1/500 - 1/2000; IF/IC: 1/10 - 1/100
Immunogen
Recombinant full length protein of human KNP-I
Preparation and Storage
Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C for one year. Avoid freeze/thaw cycles.

Western Blot (WB)

(Western blot analysis of KNP-I expression in SW480 (A), HepG2 (B), mouse skin (C), mouse heart (D), rat skeletal muscle (E) whole cell lysates.)

Western Blot (WB) (Western blot analysis of KNP-I expression in SW480 (A), HepG2 (B), mouse skin (C), mouse heart (D), rat skeletal muscle (E) whole cell lysates.)

Immunofluorescence (IF)

(Immunofluorescent analysis of KNP-I staining in HepG2 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.)

Immunofluorescence (IF) (Immunofluorescent analysis of KNP-I staining in HepG2 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.)
Related Product Information for anti-C21orf33 antibody
Rabbit polyclonal antibody to KNP-I

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
24,758 Da
NCBI Official Full Name
ES1 protein homolog, mitochondrial isoform 1
NCBI Official Synonym Full Names
chromosome 21 open reading frame 33
NCBI Official Symbol
C21orf33
NCBI Official Synonym Symbols
ES1; HES1; KNPH; KNPI; GT335
NCBI Protein Information
ES1 protein homolog, mitochondrial
UniProt Protein Name
ES1 protein homolog, mitochondrial
UniProt Gene Name
C21orf33
UniProt Synonym Gene Names
HES1; KNPI
UniProt Entry Name
ES1_HUMAN

NCBI Description

This gene encodes a potential mitochondrial protein that is a member of the DJ-1/PfpI gene family. This protein is overexpressed in fetal Down syndrome brain. Alternate splicing results in multiple transcript variants. [provided by RefSeq, May 2010]

Uniprot Description

C21orf33: a mitochondrial protein. Two alternatively spliced isoforms have been described.

Protein type: Unknown function

Chromosomal Location of Human Ortholog: 21q22.3

Cellular Component: mitochondrion

Research Articles on C21orf33

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Product Notes

The C21orf33 c21orf33 (Catalog #AAA8247278) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-KNP-I Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's KNP-I can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC). WB: 1/500 - 1/2000; IF/IC: 1/10 - 1/100. Researchers should empirically determine the suitability of the C21orf33 c21orf33 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "KNP-I, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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