Chromatin Immunoprecipitation (ChIP)
(Chromatin Immunoprecipitation (ChIP) Using Suv420h1 antibody - C-terminal region and HCT116 Cells)
Rabbit KMT5B Polyclonal Antibody | anti-KMT5B antibody
KMT5B Antibody - C-terminal region
Predicted: Cow, Dog, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat, Zebrafish
Predicted: Cow, Dog, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat, Zebrafish
Chromatin Immunoprecipitation (ChIP)
(Chromatin Immunoprecipitation (ChIP) Using Suv420h1 antibody - C-terminal region and HCT116 Cells)
Chromatin Immunoprecipitation (ChIP)
(Chromatin Immunoprecipitation (ChIP) Using Suv420h1 antibody - C-terminal region and HCT116 Cells)
Chromatin Immunoprecipitation (ChIP)
(Quiescent human colon carcinoma HCT116 cultures were treated with 10% FBS for three time points (0, 15, 30min) or (0, 30, 60min) were used in Matrix-ChIP and real-time PCR assays at EGR1 gene (Exon1) and 15kb upstream site.)
Chromatin Immunoprecipitation (ChIP)
(Quiescent human colon carcinoma HCT116 cultures were treated with 10% FBS for three time points (0, 15, 30min) or (0, 30, 60min) were used in Matrix-ChIP and real-time PCR assays at EGR1 gene (Exon1) and 15kb upstream site.)
Western Blot (WB)
(WB Suggested Anti-Suv420h1 AntibodyTitration: 1.0 ug/mlPositive Control: Mouse Thymus)
Western Blot (WB)
(WB Suggested Anti-Suv420h1 AntibodyTitration: 1.0 ug/mlPositive Control: Mouse Thymus)
NCBI and Uniprot Product Information
Uniprot Description
SUV420H1: a protein methyltransferase that specifically trimethylates 'Lys-20' of histone H4. H4 'Lys-20' trimethylation represents a specific tag for epigenetic transcriptional repression. Mainly functions in pericentric heterochromatin regions, thereby playing a central role in the establishment of constitutive heterochromatin in these regions. SUV420H1 is targeted to histone H3 via its interaction with RB1 family proteins (RB1, RBL1 and RBL2). Strongly down-regulated in breast cancer cells. Belongs to the histone-lysine methyltransferase family. Suvar4-20 subfamily. Three alternatively-spliced isoforms have been described.
Protein type: EC 2.1.1.43; Amino Acid Metabolism - lysine degradation; Methyltransferase, protein lysine; Methyltransferase
Cellular Component: chromosome; nucleus; condensed nuclear chromosome, pericentric region
Molecular Function: transferase activity; methyltransferase activity; histone lysine N-methyltransferase activity (H4-K20 specific); histone-lysine N-methyltransferase activity
Biological Process: histone methylation; methylation; muscle development; regulation of transcription, DNA-dependent; transcription, DNA-dependent; chromatin modification; peptidyl-lysine methylation
Research Articles on KMT5B
Similar Products
Product Notes
The KMT5B suv420h1 (Catalog #AAA3210270) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The KMT5B Antibody - C-terminal region reacts with Tested: Mouse Predicted: Cow, Dog, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat, Zebrafish and may cross-react with other species as described in the data sheet. AAA Biotech's KMT5B can be used in a range of immunoassay formats including, but not limited to, Chromatin IP (ChIP), Western Blot (WB). Researchers should empirically determine the suitability of the KMT5B suv420h1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Synthetic peptide located within the following region: FINHDCRPNC KFVSTGRDTA CVKALRDIEP GEEISCYYGD GFFGENNEFC. It is sometimes possible for the material contained within the vial of "KMT5B, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
