Rabbit anti-Human, Mouse KLHL3 Polyclonal Antibody | anti-KLHL3 antibody

KLHL3 Antibody

Cat. Num. AAA9412560

• Gene Names: KLHL3; PHA2D
• Reactivity: Human, Mouse
• Applications: Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Synonyms: KLHL3; Polyclonal Antibody; KLHL3 Antibody; Kelch-like protein 3; KIAA1129; anti-KLHL3 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Specificity: The antibody detects endogenous levels of total KLHL3 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Form/Format: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1.0 mg/ml (varies by lot)
• Sequence Length: 555

• Applicable Applications for anti-KLHL3 antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: Western blotting: 1:500~1:3000; Immunohistochemistry: 1:50~1:100; Immunofluorescence: 1:100~1:500
• Immunogen Type: Peptide
• Immunogen Description: Synthesized peptide derived from N-terminal of human KLHL3.
• Target Name: KLHL3
• Preparation and Storage: Store at -20 degree C

Western Blot (WB)

(Western blot analysis of extracts from RAW264.7 cells and 3T3 cells, using KLHL3 antibody.)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis of paraffin-embedded human brain tissue, using KLHL3 antibody.)

Immunofluorescence (IF)

(Immunofluorescence analysis of A549 cells, using KLHL3 antibody.)

Related Product Information for anti-KLHL3 antibody

Substrate-specific adapter of a BCR (BTB-CUL3-RBX1) E3 ubiquitin ligase complex that acts as a regulator of ion transport in the distal nephron. The BCR(KLHL3) complex acts by mediating ubiquitination of WNK4, an inhibitor of potassium channel KCNJ1, leading to WNK4 degradation.Lai F., Genomics 66:65-75(2000).Hirosawa M., DNA Res. 6:329-336(1999).Ota T., Nat. Genet. 36:40-45(2004).

Product Categories/Family for anti-KLHL3 antibody

Total protein Ab

NCBI and Uniprot Product Information

• NCBI GI #: 380692310
• NCBI GeneID: 26249
• NCBI Accession #: NP_001244123.1
• NCBI GenBank Nucleotide #: NM_001257194.1
• UniProt Accession #: Q9UH77; Q9UH75; Q9UH76; Q9ULU0; Q9Y6V6; B2RBK7
• Molecular Weight: 55,927 Da
• NCBI Official Full Name: kelch-like protein 3 isoform 2
• NCBI Official Synonym Full Names: kelch-like family member 3
• NCBI Official Symbol: KLHL3
• NCBI Official Synonym Symbols: PHA2D
• NCBI Protein Information: kelch-like protein 3
• UniProt Protein Name: Kelch-like protein 3
• Protein Family: Kelch-like protein
• UniProt Gene Name: KLHL3
• UniProt Synonym Gene Names: KIAA1129
• UniProt Entry Name: KLHL3_HUMAN

NCBI Description

This gene is ubiquitously expressed and encodes a full-length protein which has an N-terminal BTB domain followed by a BACK domain and six kelch-like repeats in the C-terminus. These kelch-like repeats promote substrate ubiquitination of bound proteins via interaction of the BTB domain with the CUL3 (cullin 3) component of a cullin-RING E3 ubiquitin ligase (CRL) complex. Muatations in this gene cause pseudohypoaldosteronism type IID (PHA2D); a rare Mendelian syndrome featuring hypertension, hyperkalaemia and metabolic acidosis. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Mar 2012]

Uniprot Description

KLHL3: Substrate-specific adapter of a BCR (BTB-CUL3-RBX1) E3 ubiquitin ligase complex that acts as a regulator of ion transport in the distal nephron. The BCR(KLHL3) complex may act by mediating ubiquitination of SLC12A3/NCC, thereby regulating SLC12A3/NCC subcellular location at the cell membrane. Defects in KLHL3 are the cause of Pseudohypoaldosteronism type 2D (PHA2D). A disorder characterized by severe hypertension, hyperkalemia, hyperchloremia, hyperchloremic metabolic acidosis, and correction of physiologic abnormalities by thiazide diuretics. PHA2D inheritance is autosomal dominant or recessive. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Cytoskeletal; Actin-binding

Chromosomal Location of Human Ortholog: 5q31

Cellular Component: cytoskeleton; cytosol

Molecular Function: protein binding; actin binding; structural molecule activity

Biological Process: ion homeostasis; protein ubiquitination during ubiquitin-dependent protein catabolic process; protein ubiquitination

Disease: Pseudohypoaldosteronism, Type Iid