Rabbit anti-Mouse, Rat Csf1r Polyclonal Antibody | anti-Csf1r antibody

Csf1r, CT (Csf1r, Csfmr, Fms, Macrophage colony-stimulating factor 1 receptor, CSF-1 receptor, Proto-oncogene c-Fms, CD115) (MaxLight 550)

Cat. Num. AAA6289194

• Gene Names: Csf1r; Fms; CD115; Csfmr; Fim-2; CSF-1R; M-CSFR; M-CSF-R; AI323359
• Reactivity: Mouse, Rat
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by Protein A and Peptide Affinity Chromatography.
• Synonyms: Csf1r; Polyclonal Antibody; CT (Csf1r; Csfmr; Fms; Macrophage colony-stimulating factor 1 receptor; CSF-1 receptor; Proto-oncogene c-Fms; CD115) (MaxLight 550); CD115; anti-Csf1r antibody

• Host: Rabbit
• Reactivity: Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes mouse Csf1r. Species Crossreactivity: rat
• Purity/Purification: Purified by Protein A and Peptide Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight550.

• Applicable Applications for anti-Csf1r antibody: Immunohistochemistry (IHC) Paraffin, FLISA, Western Blot (WB)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 895-923 in the C-terminal region of mouse Csf1r.
• Conjugate: MaxLight550
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight550 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western Blot analysis in mouse heart tissue lysates (35ug/lane) usingMBS645517. This demonstrates that MBS645517 detected the Csf1r protein (arrow).)

Western Blot (WB)

(Western Blot using MBS645517 at 1:2000 dilution Lane 1: mouse skeletal muscle lysate 20ug/lane Lane 2: rat skeletal muscle lysate 20ug/lane Lane 3: mouse heart lysate 20ug/lane. Secondary IgG, (H+L) (HRP) goat anti-rabbit. Predicted band size: 109kD Blocking/Dilution buffer: 5% NFDM/TBST.)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis in formalin fixed and paraffin embedded mouse cerebellum tissue using MBS645517 followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of MBS645517 for immunohistochemistry.)

Product Categories/Family for anti-Csf1r antibody

Antibodies; CD Markers

References

1. Gueller, S., et al. J. Leukoc. Biol. 88(4):699-706 (2010). 2. Nagamachi, A., et al. Dev. Biol. 345(2):226-236 (2010). 3. Wei, S., et al. J. Leukoc. Biol. 88(3):495-505 (2010). 4. Maitra, R., et al. J. Immunol. 185(3):1485-1491 (2010). 5. Aikawa, Y., et al. Nat. Med. 16(5):580-585 (2010).

NCBI and Uniprot Product Information

• NCBI GI #: 126723423
• NCBI GeneID: 12978
• NCBI Accession #: NP_001032948.2
• NCBI GenBank Nucleotide #: NM_001037859.2
• UniProt Accession #: P09581; Q3U3P1; Q9DBH9
• Molecular Weight: 109,179 Da
• NCBI Official Full Name: macrophage colony-stimulating factor 1 receptor
• NCBI Official Synonym Full Names: colony stimulating factor 1 receptor
• NCBI Official Symbol: Csf1r
• NCBI Official Synonym Symbols: Fms; CD115; Csfmr; Fim-2; CSF-1R; M-CSFR; M-CSF-R; AI323359
• NCBI Protein Information: macrophage colony-stimulating factor 1 receptor; CSF-1 receptor; CSF-1-R; c-fms; proto-oncogene c-Fms; proto-oncogene fms
• UniProt Protein Name: Macrophage colony-stimulating factor 1 receptor
• Protein Family: Macrophage colony-stimulating factor 1 receptor
• UniProt Gene Name: Csf1r
• UniProt Synonym Gene Names: Csfmr; Fms; CSF-1-R; CSF-1R; M-CSF-R
• UniProt Entry Name: CSF1R_MOUSE

Uniprot Description

CSFR: an oncogenic tyrosine kinase receptor for CSF-1 (M-CSF). Drives growth and development of monocytes. Binding of CSF-1 induces receptor dimerization, activation and autophosphorylation of cytoplasmic tyrosine residues used as docking sites for SH2-containing signaling proteins. There are at least five major tyrosine autophosphorylation sites. Two point mutations seen in 10-20% of patients with acute myeloid leukemia, chronic myelomonocytic leukemia or myelodysplasia. One mutation appears to be both somatic and germline, and disrupts Cbl binding and receptor turnover. v-fms lacks the Cbl binding site and causes feline leukemia. Mutations may also develop after chemotherapy for lymphoma. A distinct point mutation was found in some cases of hepatocellular carcinoma and related to increased expression, and another mutation was found in 2 of 40 patients with idiopathic myelofibrosis. Expression is elevated in breast tumors and cell lines, and expression in xenografts and transgenic mice has been correlated with xenograft growth and breast cancer development. Inhibitors: Ki-20227 and other Kit/PDGFR inhibitors.

Protein type: Protein kinase, TK; Oncoprotein; EC 2.7.10.1; Protein kinase, tyrosine (receptor); Membrane protein, integral; Kinase, protein; TK group; PDGFR family

Cellular Component: cell surface; membrane; plasma membrane; integral to membrane; intracellular; receptor complex

Molecular Function: transferase activity; macrophage colony stimulating factor receptor activity; protein binding; protein homodimerization activity; protein-tyrosine kinase activity; cytokine binding; transferase activity, transferring phosphorus-containing groups; nucleotide binding; kinase activity; transmembrane receptor protein tyrosine kinase activity; protein phosphatase binding; ATP binding; protein kinase activity

Biological Process: regulation of bone resorption; phosphoinositide-mediated signaling; peptidyl-tyrosine phosphorylation; positive regulation of osteoclast differentiation; immune system process; cytokine and chemokine mediated signaling pathway; protein amino acid autophosphorylation; osteoclast differentiation; protein amino acid phosphorylation; positive regulation of tyrosine phosphorylation of Stat3 protein; cell proliferation; regulation of cell shape; positive regulation of cell proliferation; ruffle organization and biogenesis; innate immune response; hemopoiesis; positive regulation of protein amino acid phosphorylation; phosphatidylinositol metabolic process; inflammatory response; intercellular junction maintenance; phosphorylation; transmembrane receptor protein tyrosine kinase signaling pathway; positive regulation of cell migration