anti-Human KLHL3 Antibody | anti-KLHL3 antibody

KLHL3 Antibody

Cat. Num. AAA853527

• Gene Names: KLHL3; PHA2D
• Reactivity: Human
• Applications: Western Blot, Immunohistochemistry, Immunofluorescence, ELISA
• Purity: Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Synonyms: KLHL3; KLHL3 Antibody; Kelch-like protein 3; KIAA1129; anti-KLHL3 antibody

• Reactivity: Human
• Specificity: KLHL3 antibody detects endogenous levels of total KLHL3 protein.
• Purity/Purification: Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Form/Format: In phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Sequence Length: 555

• Applicable Applications for anti-KLHL3 antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA (EIA)
• Application Notes: WB: 1:500~1:1000; IHC: 1:50~1:100; IF: 1:100~1:500; ELISA: 1:20000
• Immunogen: The antiserum was produced against synthesized peptide derived from N-terminal of human KLHL3.
• Preparation and Storage: Store at-20 degree C for 1 year.

Testing Data

(Peptide-+)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis of paraffin-embedded human brain tissue, using KLHL3 antibody. Western blot analysis of extracts from RAW264.7 cells and 3T3 cells, using KLHL3 antibody.)

Immunofluorescence (IF)

(Peptide-+ Immunofluorescence analysis of A549 cells, using KLHL3 antibody.)

Product Categories/Family for anti-KLHL3 antibody

Autophagy antibody; Cancer; Cardiovascular; Cell Biology; Epigenetics & Nuclear Signaling; Developmental Biologys; Immunology; Drug Discovery Products; Metabolism; Neuroscience; Signal Transduction; Stem Cells

References

Lai F., Genomics 66:65-75(2000). Hirosawa M., DNA Res. 6:329-336(1999). Ota T., Nat. Genet. 36:40-45(2004).

NCBI and Uniprot Product Information

• NCBI GI #: 380692310
• NCBI GeneID: 26249
• NCBI Accession #: NP_001244123.1
• NCBI GenBank Nucleotide #: NM_001257194.1
• UniProt Accession #: Q9UH77; Q9UH75; Q9UH76; Q9ULU0; Q9Y6V6; B2RBK7
• Molecular Weight: 55,927 Da
• NCBI Official Full Name: kelch-like protein 3 isoform 2
• NCBI Official Synonym Full Names: kelch like family member 3
• NCBI Official Symbol: KLHL3
• NCBI Official Synonym Symbols: PHA2D
• NCBI Protein Information: kelch-like protein 3
• UniProt Protein Name: Kelch-like protein 3
• Protein Family: Kelch-like protein
• UniProt Gene Name: KLHL3

NCBI Description

This gene is ubiquitously expressed and encodes a full-length protein which has an N-terminal BTB domain followed by a BACK domain and six kelch-like repeats in the C-terminus. These kelch-like repeats promote substrate ubiquitination of bound proteins via interaction of the BTB domain with the CUL3 (cullin 3) component of a cullin-RING E3 ubiquitin ligase (CRL) complex. Muatations in this gene cause pseudohypoaldosteronism type IID (PHA2D); a rare Mendelian syndrome featuring hypertension, hyperkalaemia and metabolic acidosis. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Mar 2012]

Uniprot Description

Substrate-specific adapter of a BCR (BTB-CUL3-RBX1) E3 ubiquitin ligase complex that acts as a regulator of ion transport in the distal nephron (PubMed:14528312, PubMed:22406640, PubMed:23387299, PubMed:23453970, PubMed:23576762, PubMed:23665031). The BCR(KLHL3) complex acts by mediating ubiquitination of WNK4, an inhibitor of potassium channel KCNJ1, leading to WNK4 degradation (PubMed:23387299, PubMed:23453970, PubMed:23576762, PubMed:23665031). The BCR(KLHL3) complex also mediates ubiquitination and degradation of CLDN8, a tight-junction protein required for paracellular chloride transport in the kidney ().