Immunohistochemistry (IHC)
(Anti-KLHL25 antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)
Chicken anti-Human KLHL25 Polyclonal Antibody | anti-KLHL25 antibody
Chicken Polyclonal to Human KLHL25
Usage: ENC-2 antibody can be used for detection of ENC-2 by Western blot at 1-2 ug/ml.
Immunohistochemistry (IHC)
(Anti-KLHL25 antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)
Immunohistochemistry (IHC)
(Anti-KLHL25 antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)
Immunohistochemistry (IHC)
(Anti-KLHL25 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)
Immunohistochemistry (IHC)
(Anti-KLHL25 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)
Immunohistochemistry (IHC)
(Anti-KLHL25 antibody IHC of human thymus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)
Immunohistochemistry (IHC)
(Anti-KLHL25 antibody IHC of human thymus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)
Immunofluorescence (IF)
(Immunofluorescence of ENC-2 in Jurkat cells with ENC-2 antibody at 20 ug/ml.)
Immunofluorescence (IF)
(Immunofluorescence of ENC-2 in Jurkat cells with ENC-2 antibody at 20 ug/ml.)
Immunocytochemistry (ICC)
(Immunocytochemistry of ENC-2 in Jurkat cells with ENC-2 antibody at 5 ug/ml.)
Immunocytochemistry (ICC)
(Immunocytochemistry of ENC-2 in Jurkat cells with ENC-2 antibody at 5 ug/ml.)
Western Blot (WB)
(Western blot of ENC-2 in Jurkat cell lysate with ENC-2 antibody at 1 ug/ml in (A) the absence and (B) the presence of blocking peptide.)
Western Blot (WB)
(Western blot of ENC-2 in Jurkat cell lysate with ENC-2 antibody at 1 ug/ml in (A) the absence and (B) the presence of blocking peptide.)
NCBI and Uniprot Product Information
Uniprot Description
KLHL25: Substrate-specific adapter of a BCR (BTB-CUL3-RBX1) E3 ubiquitin ligase complex required for translational homeostasis. The BCR(KLHL25) ubiquitin ligase complex acts by mediating ubiquitination of hypophosphorylated EIF4EBP1 (4E-BP1): ubiquitination and subsequent degradation of hypophosphorylated EIF4EBP1 (4E-BP1) probably serves as a homeostatic mechanism to maintain translation and prevent eIF4E inhibition when eIF4E levels are low. The BCR(KLHL25) complex does not target EIF4EBP1 (4E-BP1) when it is hyperphosphorylated or associated with eIF4E. Component of the BCR(KLHL25) E3 ubiquitin ligase complex, at least composed of CUL3, KLHL25 and RBX1. Interacts with EIF4EBP1 (when hypophosphorylated)
Protein type: Unknown function
Chromosomal Location of Human Ortholog: 15q25.3
Cellular Component: cytoplasm
Biological Process: protein ubiquitination during ubiquitin-dependent protein catabolic process; protein ubiquitination; regulation of translational initiation
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Product Notes
The KLHL25 klhl25 (Catalog #AAA244320) is an Antibody produced from Chicken and is intended for research purposes only. The product is available for immediate purchase. The Chicken Polyclonal to Human KLHL25 reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's KLHL25 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA). ELISA, IHC-P (5 ug/ml), WB (1 - 2 ug/ml) Usage: ENC-2 antibody can be used for detection of ENC-2 by Western blot at 1-2 ug/ml. Researchers should empirically determine the suitability of the KLHL25 klhl25 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "KLHL25, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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