Rabbit KLF4 Polyclonal Antibody | anti-KLF4 antibody

KLF4 Polyclonal Antibody

Cat. Num. AAA3012957

• Gene Names: KLF4; EZF; GKLF
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry
• Purity: ProA affinity purified
• Synonyms: KLF4; Polyclonal Antibody; KLF4 Polyclonal Antibody; Endothelial Kruppel like zinc finger protein; Epithelial zinc finger protein EZF; EZF; GKLF; gut Kruppel-like factor; Gut-enriched krueppel-like factor; KLF; Krueppel-like factor 4; Kruppel like factor 4 (Epithelial zinc finger protein EZF) (Gut enriched Krueppel like factor); Kruppel like factor 4 (gut); anti-KLF4 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: KLF4 polyclonal antibody detects endogenous levels of KLF4 protein.
• Purity/Purification: ProA affinity purified
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 479

• Applicable Applications for anti-KLF4 antibody: Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC)
• Application Notes: WB: 1:1,000-1:5,000; ICC: 1:50-1:200; IHC: 1:50-1:200
• Immunogen: recombinant protein
• Preparation and Storage: Store at 4 degree C after thawing.; Aliquot store at -20 degree C or -80 degree C.; Avoid repeated freeze/thaw cycles.

Western Blot (WB)

(Western Blot analysis of KLF4 on different lysates using anti-KLF4 antibody at 1/1,000 dilution. Positive control: Lane 1: 293 Lane 2: NCCIT)

Immunocytochemistry (ICC)

(ICC staining KLF4 in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

Related Product Information for anti-KLF4 antibody

The Kruppel-type zinc finger transcription factors comprise a conserved family of DNA binding proteins that are important in developmental regulation. The Kruppel zinc finger transcription factor was initially identified in Drosophila as a segmentation gene. Kruppel-like factors that have been characterized in mammals include EKLF, LKLF and GKLF (6-8). EKLF is expressed principally in erythroid tissues, and LKLF expression is limited to the lung. GKLF is found predominantly in gut and has been shown to be expressed during growth arres.

NCBI and Uniprot Product Information

• NCBI GI #: 194248077
• NCBI GeneID: 9314
• NCBI Accession #: NP_004226.3
• NCBI GenBank Nucleotide #: NP_004226.3
• UniProt Accession #: O43474; Q60793
• Molecular Weight: 55kDa
• NCBI Official Full Name: Krueppel-like factor 4 isoform 2
• NCBI Official Synonym Full Names: Kruppel like factor 4
• NCBI Official Symbol: KLF4
• NCBI Official Synonym Symbols: EZF; GKLF
• NCBI Protein Information: Krueppel-like factor 4
• UniProt Protein Name: Krueppel-like factor 4
• Protein Family: Krueppel-like factor
• UniProt Gene Name: KLF4
• UniProt Synonym Gene Names: EZF; GKLF
• UniProt Entry Name: KLF4_HUMAN

NCBI Description

This gene encodes a protein that belongs to the Kruppel family of transcription factors. The encoded zinc finger protein is required for normal development of the barrier function of skin. The encoded protein is thought to control the G1-to-S transition of the cell cycle following DNA damage by mediating the tumor suppressor gene p53. Mice lacking this gene have a normal appearance but lose weight rapidly, and die shortly after birth due to fluid evaporation resulting from compromised epidermal barrier function. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Sep 2015]