IFN-omega Recombinant Protein | IFNW1 recombinant protein

IFN-omega Protein, Human, Recombinant (hFc Tag)

Cat. Num. AAA8118616

• Purity: >92 % as determined by SDS-PAGE
• Synonyms: IFN-omega; IFN-omega Protein; Human; Recombinant (hFc Tag); Human Interferon omega-1/IFNomega/IFNW1 Protein (Fc Tag); interferon; omega 1; IFN-omega 1 Protein; IFNW1 Protein; interferon omega-1 Protein; IFNW1 recombinant protein

• Host: HEK293 Cells
• Purity/Purification: >92 % as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4 Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Sequence Positions: 409aa
• Sequence: Cys24-Ser195

• Species: Human
• Tag: hFc
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Glu20
• Protein Construction: |A DNA sequence encoding the human IFNomega (NP_002168.1) (Cys 24-Ser 195) was fused with the Fc region of human IgG1 at the N-terminus
• Activity: Measured in antiviral assays using WISH human amnion cells infected with vesicular stomatitis virus (VSV). The EC50 for this effect is 0.03-0.3ng/mL.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; Shipping: They are shipped out with blue ice pack

Testing Data

Testing Data

(Measured in antiviral assays using WISH human amnion cells infected with vesicular stomatitis virus (VSV). The EC50 for this effect is 0.03-0.3 ng/mL.)

Related Product Information for IFNW1 recombinant protein

IFNs are a large family of proteins having antiviral, antiproliferative, and immunomodulatory effects, and are divided into two major classes, type I and type II, based on differences in receptor binding and nucleotide sequence. Type I IFNs consist of IFN alpha, beta, tau, and omega and bind to the type I IFN receptor, whereas IFN-gamma is the only type II IFN and is specific for the type II IFN receptor. Human IFN-omega, was identified by three independent groups in 1985 and is structurally related to IFN-alpha and -beta. Both human IFN-omega and IFN-alpha are produced by virally induced leukocytes and have similar antiviral activities on human cell lines, and a sizeable proportion (at least 1%) of the total antiviral activity of leukocyte IFN is contributed by IFN-omegal. Also, it was reported that IFN-omega could inhibit the growth of human tumors in vivo.

References

Capon D.J., et al., (1985), Two distinct families of human and bovine interferon-alpha genes are coordinately expressed and encode functional polypeptides. Mol. Cell. Biol. 5:768-779.Adolf G.R., et al., (1991), Human interferon omega 1: isolation of the gene, expression in Chinese hamster ovary cells and characterization of the recombinant protein.Biochim. Biophys. Acta 1089:167-174.Humphray S.J., et al., (2004), DNA sequence and analysis of human chromosome 9.Nature 429:369-374.

NCBI and Uniprot Product Information

• NCBI GI #: 4504605
• NCBI GeneID: 3467
• NCBI Accession #: NP_002168.1
• NCBI GenBank Nucleotide #: NM_002177.1
• UniProt Accession #: P05000; Q13168; Q5U802; Q5VWD0; Q7M4P5
• Molecular Weight: 22,319 Da
• NCBI Official Full Name: interferon omega-1
• NCBI Official Synonym Full Names: interferon, omega 1
• NCBI Official Symbol: IFNW1
• NCBI Protein Information: interferon omega-1; IFN-omega 1, interferon omega-1; interferon alpha-II-1
• UniProt Protein Name: Interferon omega-1
• Protein Family: Interferon
• UniProt Gene Name: IFNW1
• UniProt Entry Name: IFNW1_HUMAN

Uniprot Description

IFNW1: Belongs to the alpha/beta interferon family.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 9p22

Cellular Component: extracellular space

Molecular Function: hematopoietin/interferon-class (D200-domain) cytokine receptor binding; interferon-alpha/beta receptor binding; cytokine activity

Biological Process: B cell proliferation; adaptive immune response; response to virus; cytokine and chemokine mediated signaling pathway; regulation of MHC class I biosynthetic process; humoral immune response; T cell activation during immune response; response to exogenous dsRNA; B cell differentiation; natural killer cell activation during immune response; innate immune response; cell cycle arrest; defense response to virus; positive regulation of peptidyl-serine phosphorylation of STAT protein