Rabbit anti-Human, Mouse KLF12 Polyclonal Antibody | anti-KLF12 antibody

KLF12 Rabbit pAb

Cat. Num. AAA4755141

• Gene Names: KLF12; AP2REP; AP-2rep; HSPC122
• Reactivity: Human, Mouse
• Applications: Western Blot, Immunohistochemistry, ELISA
• Purity: Affinity purified
• Synonyms: KLF12; Polyclonal Antibody; KLF12 Rabbit pAb; Krueppel-like factor 12; AP2REP; HSPC122; Transcriptional repressor AP-2rep; anti-KLF12 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Specificity: KLF12 Polyclonal Antibody detects endogenous levels of KLF12 protein.
• Purity/Purification: Affinity purified
• Form/Format: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
• Concentration: 1mg/ml (varies by lot)

• Applicable Applications for anti-KLF12 antibody: Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), ELISA (EIA)
• Application Notes: WB: 1:500-1:2000; IHC-P: 1:100-300; ELISA: 1:20,000
• Immunogen: Synthesized peptide derived from the Internal region of human KLF12.
• Conjugation: Unconjugated
• Nucleotide Accession: 11278
• Modification: Unmodified
• Preparation and Storage: Store at 4 degree C for short term. Store at -20 degree C for long term. Avoid freeze/thaw cycle.

Western Blot (WB)

(Western Blot analysis of rat muscle cells using KLF12 Polyclonal Antibody. Secondary antibody was diluted at 1:20000)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded mouse-brain, antibody was diluted at 1:100)

Product Categories/Family for anti-KLF12 antibody

Primary antibody

NCBI and Uniprot Product Information

• NCBI GI #: 91771555
• NCBI GeneID: 11278
• UniProt Accession #: Q9Y4X4; Q5VZM7; Q9UHZ0; A8K5T2; L0R3J4
• Molecular Weight: 402
• NCBI Official Full Name: Krueppel-like factor 12
• NCBI Official Synonym Full Names: Kruppel-like factor 12
• NCBI Official Symbol: KLF12
• NCBI Official Synonym Symbols: AP2REP; AP-2rep; HSPC122
• NCBI Protein Information: Krueppel-like factor 12; AP-2 repressor; AP-2rep transcription factor; transcriptional repressor AP-2rep; KLF12 zinc finger transcriptional repressor
• UniProt Protein Name: Krueppel-like factor 12
• Protein Family: Krueppel-like factor
• UniProt Gene Name: KLF12
• UniProt Synonym Gene Names: AP2REP
• UniProt Entry Name: KLF12_HUMAN

NCBI Description

Activator protein-2 alpha (AP-2 alpha) is a developmentally-regulated transcription factor and important regulator of gene expression during vertebrate development and carcinogenesis. The protein encoded by this gene is a member of the Kruppel-like zinc finger protein family and can repress expression of the AP-2 alpha gene by binding to a specific site in the AP-2 alpha gene promoter. Repression by the encoded protein requires binding with a corepressor, CtBP1. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

KLF12: Confers strong transcriptional repression to the AP-2- alpha gene. Binds to a regulatory element (A32) in the AP-2-alpha gene promoter. Belongs to the Sp1 C2H2-type zinc-finger protein family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; C2H2-type zinc finger protein; DNA-binding

Chromosomal Location of Human Ortholog: 13q22

Cellular Component: nucleus

Molecular Function: DNA binding; metal ion binding; transcription corepressor activity; transcription factor activity

Biological Process: regulation of transcription from RNA polymerase II promoter; transcription, DNA-dependent; positive regulation of transcription from RNA polymerase II promoter; negative regulation of transcription from RNA polymerase II promoter