Western Blot (WB)
(TIGAR Antibody (N-term) western blot analysis in mouse kidney tissue lysates (35ug/lane).This demonstrates the TIGAR antibody detected the TIGAR protein (arrow).)
Rabbit anti-Mouse TIGAR Polyclonal Antibody | anti-C5H12orf5 antibody
TIGAR, NT (TIGAR, C12orf5, Fructose-2,6-bisphosphatase TIGAR, TP53-induced glycolysis and apoptosis regulator)
Gene Names
C5H12orf5; TIGAR
Reactivity
Mouse
Applications
ELISA, Western Blot
Purity
Affinity PurifiedPurified by Protein A affinity chromatography.
Synonyms
TIGAR; Polyclonal Antibody; NT (TIGAR; C12orf5; Fructose-2; 6-bisphosphatase TIGAR; TP53-induced glycolysis and apoptosis regulator); Anti -TIGAR; anti-C5H12orf5 antibody
Host
Rabbit
Reactivity
Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
Mouse
Purity/Purification
Affinity Purified
Purified by Protein A affinity chromatography.
Purified by Protein A affinity chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Applicable Applications for anti-C5H12orf5 antibody
ELISA (EL/EIA), Western Blot (WB)
Application Notes
Suitable for use in Western Blot, ELISA
Dilution: ELISA: 1:1,000
Western Blot: 1:100-500
Dilution: ELISA: 1:1,000
Western Blot: 1:100-500
Immunogen
TIGAR antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human TIGAR.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Western Blot (WB)
(TIGAR Antibody (N-term) western blot analysis in mouse kidney tissue lysates (35ug/lane).This demonstrates the TIGAR antibody detected the TIGAR protein (arrow).)
Western Blot (WB)
(TIGAR Antibody (N-term) western blot analysis in mouse kidney tissue lysates (35ug/lane).This demonstrates the TIGAR antibody detected the TIGAR protein (arrow).)
Related Product Information for anti-C5H12orf5 antibody
This gene is regulated as part of the p53 tumor suppressor pathway and encodes a protein with sequence similarity to the bisphosphate domain of the glycolytic enzyme that degrades fructose-2,6-bisphosphate. The protein functions by blocking glycolysis and directing the pathway into the pentose phosphate shunt. Expression of this protein also protects cells from DNA damaging reactive oxygen species and provides some protection from DNA damage-induced apoptosis. The 12p13.32 region that includes this gene is paralogous to the 11q13.3 region. [provided by RefSeq].
Product Categories/Family for anti-C5H12orf5 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
29,467 Da
NCBI Official Full Name
TPA: probable fructose-2,6-bisphosphatase TIGAR
NCBI Official Symbol
C5H12orf5
NCBI Official Synonym Symbols
TIGAR
NCBI Protein Information
fructose-2,6-bisphosphatase TIGAR; probable fructose-2,6-bisphosphatase TIGAR; TP53-induced glycolysis and apoptosis regulator
UniProt Protein Name
Fructose-2,6-bisphosphatase TIGAR
UniProt Gene Name
TIGAR
UniProt Entry Name
TIGAR_BOVIN
Uniprot Description
Function: Fructose-bisphosphatase hydrolyzing fructose-2,6-bisphosphate as well as fructose-1,6-bisphosphate. Inhibits glycolysis by reducing cellular levels of fructose-2,6-bisphosphate. May protect cells against reactive oxygen species and against apoptosis induced by tp53
By similarity.
Catalytic activity: Beta-D-fructose 2,6-bisphosphate + H2O = D-fructose 6-phosphate + phosphate.
Subunit structure: Monomer
By similarity.
Sequence similarities: Belongs to the phosphoglycerate mutase family.
Caution: Not expected to have any kinase activity.
Similar Products
Product Notes
The C5H12orf5 tigar (Catalog #AAA6000931) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The TIGAR, NT (TIGAR, C12orf5, Fructose-2,6-bisphosphatase TIGAR, TP53-induced glycolysis and apoptosis regulator) reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's TIGAR can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB). Suitable for use in Western Blot, ELISA Dilution: ELISA: 1:1,000 Western Blot: 1:100-500. Researchers should empirically determine the suitability of the C5H12orf5 tigar for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TIGAR, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
