Immunofluorescence (IF)
(Immunofluorescence analysis of HeLa cells, using Kir5.1 (Phospho-Ser417) Antibody. The picture on the right is blocked with the phospho peptide.)
Rabbit anti-Mouse, Rat Kir5.1 Polyclonal Antibody | anti-Kir5.1 antibody
Kir5.1 (Phospho-Ser417) Antibody
Gene Names
Kcnj16; Kir5.1; AI132396; 6430410F18Rik
Reactivity
Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, ELISA
Purity
Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Synonyms
Kir5.1; Polyclonal Antibody; Kir5.1 (Phospho-Ser417) Antibody; IRK16; IRKG; Inward rectifier K channel Kir5.1; KCNJ16; Potassium channel inwardly rectifying subfamily J member 16; anti-Kir5.1 antibody
Host
Rabbit
Reactivity
Mouse, Rat
Clonality
Polyclonal
Specificity
Kir5.1 (Phospho-Ser417) Antibody detects endogenous levels of Kir5.1 only when phosphorylated at Ser417.
Purity/Purification
Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Form/Format
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1 mg/ml (varies by lot)
Sequence Length
419
Applicable Applications for anti-Kir5.1 antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA (EIA)
Application Notes
WB: 1:500~1:1000
IHC: 1:50~1:100
IF: 1:100~1:500
ELISA: 1:5000
IHC: 1:50~1:100
IF: 1:100~1:500
ELISA: 1:5000
Target Modification
Phospho
Modification Sites
M:S417; R:S417
Immunogen
The antiserum was produced against synthesized peptide derived from mouse Kir5.1 around the phosphorylation site of Ser417.
Immunogen Range
369-418
Preparation and Storage
Stable at -20 degree C for at least 1 year.
Immunofluorescence (IF)
(Immunofluorescence analysis of HeLa cells, using Kir5.1 (Phospho-Ser417) Antibody. The picture on the right is blocked with the phospho peptide.)
Immunofluorescence (IF)
(Immunofluorescence analysis of HeLa cells, using Kir5.1 (Phospho-Ser417) Antibody. The picture on the right is blocked with the phospho peptide.)
Immunohistochemistry (IHC)
(Immunohistochemistry analysis of paraffin-embedded human brain, using Kir5.1 (Phospho-Ser417) Antibody. The picture on the right is blocked with the phospho peptide.)
Immunohistochemistry (IHC)
(Immunohistochemistry analysis of paraffin-embedded human brain, using Kir5.1 (Phospho-Ser417) Antibody. The picture on the right is blocked with the phospho peptide.)
Western Blot (WB)
(Western blot analysis of lysates from RAW264.7 cells treated with forskolin 40nM 30', using Kir5.1 (Phospho-Ser417) Antibody. The lane on the right is blocked with the phospho peptide.)
Western Blot (WB)
(Western blot analysis of lysates from RAW264.7 cells treated with forskolin 40nM 30', using Kir5.1 (Phospho-Ser417) Antibody. The lane on the right is blocked with the phospho peptide.)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
47 kDa
NCBI Official Full Name
inward rectifier potassium channel 16
NCBI Official Synonym Full Names
potassium inwardly-rectifying channel, subfamily J, member 16
NCBI Official Symbol
Kcnj16
NCBI Official Synonym Symbols
Kir5.1; AI132396; 6430410F18Rik
NCBI Protein Information
inward rectifier potassium channel 16
UniProt Protein Name
Inward rectifier potassium channel 16
UniProt Gene Name
Kcnj16
Uniprot Description
Inward rectifier potassium channels are characterized by a greater tendency to allow potassium to flow into the cell rather than out of it. Their voltage dependence is regulated by the concentration of extracellular potassium; as external potassium is raised, the voltage range of the channel opening shifts to more positive voltages. The inward rectification is mainly due to the blockage of outward current by internal magnesium. KCNJ16 may be involved in the regulation of fluid and pH balance. In the kidney, together with KCNJ10, mediates basolateral K+ recycling in distal tubules; this process is critical for Na+ reabsorption at the tubules.
Research Articles on Kir5.1
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Product Notes
The Kir5.1 kcnj16 (Catalog #AAA9502854) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Kir5.1 (Phospho-Ser417) Antibody reacts with Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Kir5.1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA (EIA). WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:5000. Researchers should empirically determine the suitability of the Kir5.1 kcnj16 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Kir5.1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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