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Western Blot (WB) (Western blot analysis of extracts from RAW264.7 cells, treated with forskolin (40nM, 30mins), using Kir5.1 (Phospho-Ser417) antibody.)

anti-Mouse, Rat Kir5.1 Antibody | anti-Kir5.1 antibody

Kir5.1 (Phospho-Ser417) Antibody

Gene Names
KCNJ16; BIR9; KIR5.1
Reactivity
Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, ELISA
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Synonyms
Kir5.1; Kir5.1 (Phospho-Ser417) Antibody; IRK16; IRKG; Inward rectifier K channel Kir5.1; KCNJ16; Potassium channel; inwardly rectifying; subfamily J; member 16; anti-Kir5.1 antibody
Ordering
For Research Use Only!
Reactivity
Mouse, Rat
Specificity
Kir5.1 (Phospho-Ser417) antibody detects endogenous levels of Kir5.1 only when phosphorylated at serine 417.
Purity/Purification
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Form/Format
In phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Sequence Length
418
Applicable Applications for anti-Kir5.1 antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA (EIA)
Application Notes
WB: 1:500~1:1000
IHC: 1:50~1:100
IF: 1:100~1:500
ELISA: 1:5000
Immunogen
The antiserum was produced against synthesized phosphopeptide derived from human Kir5.1 around the phosphorylation site of serine 417 (M-E-SP-Q-M).
All Sites
Mouse: Ser417
Rat: Ser417
Preparation and Storage
Store at-20 degree C for 1 year.

Western Blot (WB)

(Western blot analysis of extracts from RAW264.7 cells, treated with forskolin (40nM, 30mins), using Kir5.1 (Phospho-Ser417) antibody.)

Western Blot (WB) (Western blot analysis of extracts from RAW264.7 cells, treated with forskolin (40nM, 30mins), using Kir5.1 (Phospho-Ser417) antibody.)
References
Liu Y., Cytogenet. Cell Genet. 90:60-63(2000). Derst C., FEBS Lett. 491:305-311(2001).

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
47,949 Da
NCBI Official Full Name
inward rectifier potassium channel 16 isoform b
NCBI Official Synonym Full Names
potassium voltage-gated channel subfamily J member 16
NCBI Official Symbol
KCNJ16
NCBI Official Synonym Symbols
BIR9; KIR5.1
NCBI Protein Information
inward rectifier potassium channel 16
UniProt Protein Name
Inward rectifier potassium channel 16
UniProt Gene Name
KCNJ16

NCBI Description

Potassium channels are present in most mammalian cells, where they participate in a wide range of physiologic responses. The protein encoded by this gene is an integral membrane protein and inward-rectifier type potassium channel. The encoded protein, which tends to allow potassium to flow into rather than out of a cell, can form heterodimers with two other inward-rectifier type potassium channels. It may function in fluid and pH balance regulation. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Apr 2014]

Uniprot Description

Inward rectifier potassium channels are characterized by a greater tendency to allow potassium to flow into the cell rather than out of it. Their voltage dependence is regulated by the concentration of extracellular potassium; as external potassium is raised, the voltage range of the channel opening shifts to more positive voltages. The inward rectification is mainly due to the blockage of outward current by internal magnesium. KCNJ16 may be involved in the regulation of fluid and pH balance. In the kidney, together with KCNJ10, mediates basolateral K+ recycling in distal tubules; this process is critical for Na+ reabsorption at the tubules (PubMed:24561201).

Research Articles on Kir5.1

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Product Notes

The Kir5.1 kcnj16 (Catalog #AAA858554) is an Antibody and is intended for research purposes only. The product is available for immediate purchase. The Kir5.1 (Phospho-Ser417) Antibody reacts with Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Kir5.1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA (EIA). WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:5000. Researchers should empirically determine the suitability of the Kir5.1 kcnj16 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Kir5.1, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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