Rabbit anti-Human KIR2DL2 Polyclonal Antibody | anti-KIR2DL2 antibody

KIR2DL2, CT (KIR2DL2, CD158B1, NKAT6, Killer cell immunoglobulin-like receptor 2DL2, CD158 antigen-like family member B1, MHC class I NK cell receptor, Natural killer-associated transcript 6, p58 natural killer cell receptor clone CL-43, CD158b1) (APC)

Cat. Num. AAA6313370

• Gene Names: KIR2DL2; NKAT6; p58.2; CD158b; NKAT-6; CD158B1
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: KIR2DL2; Polyclonal Antibody; CT (KIR2DL2; CD158B1; NKAT6; Killer cell immunoglobulin-like receptor 2DL2; CD158 antigen-like family member B1; MHC class I NK cell receptor; Natural killer-associated transcript 6; p58 natural killer cell receptor clone CL-43; CD158b1) (APC); CD158b1; anti-KIR2DL2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).

• Applicable Applications for anti-KIR2DL2 antibody: Flow Cytometry (FC/FACS), FLISA, Western Blot (WB)
• Application Notes: FC/FACS: 1:10-50; FLISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: KIR2DL2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 270-299 amino acids from the C-terminal region of human KIR2DL2.
• Conjugate: APC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(KIR2DL2 Antibody (C-Term) western blot analysis in MDA-MB231,Jurkat cell line lysates (35ug/lane).This demonstrates the KIR2DL2 antibody detected the KIR2DL2 protein (arrow).)

Flow Cytometry (FC/FACS)

(KIR2DL2 Antibody (C-Term) flow cytometric analysis of MDA-MB231 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-KIR2DL2 antibody

Killer cell immunoglobulin-like receptors (KIRs) are transmembrane glycoproteins expressed by natural killer cells and subsets of T cells. The KIR genes are polymorphic and highly homologous and they are found in a cluster on chromosome 19q13.4 within the 1 Mb leukocyte receptor complex (LRC). The gene content of the KIR gene cluster varies among haplotypes, although several 'framework' genes are found in all haplotypes (KIR3DL3, KIR3DP1, KIR3DL4, KIR3DL2). The KIR proteins are classified by the number of extracellular immunoglobulin domains (2D or 3D) and by whether they have a long (L) or short (S) cytoplasmic domain. KIR proteins with the long cytoplasmic domain transduce inhibitory signals upon ligand binding via an immune tyrosine-based inhibitory motif (ITIM), while KIR proteins with the short cytoplasmic domain lack the ITIM motif and instead associate with the TYRO protein tyrosine kinase binding protein to transduce activating signals. The ligands for several KIR proteins are subsets of HLA class I molecules; thus, KIR proteins are thought to play an important role in regulation of the immune response.

Product Categories/Family for anti-KIR2DL2 antibody

Antibodies; CD Markers

References

Jiao, Y.L., et al. J. Clin. Immunol. 30(6):840-844(2010). Zhu, B.F., et al. Hum. Immunol. 71(11):1116-1123(2010). Gao, X., et al. Clin. Immunol. 137(1):139-146(2010). Al Omar, S., et al. Hum. Immunol. 71(10):976-981(2010). Velickovic, M., et al. Tissue Antigens (2010).

NCBI and Uniprot Product Information

• NCBI GI #: 134304833
• NCBI GeneID: 3803
• NCBI Accession #: NP_055034.2
• NCBI GenBank Nucleotide #: NM_014219.2
• UniProt Accession #: P43627; Q14951; C6EVR8
• Molecular Weight: 38,472 Da
• NCBI Official Full Name: killer cell immunoglobulin-like receptor 2DL2
• NCBI Official Synonym Full Names: killer cell immunoglobulin-like receptor, two domains, long cytoplasmic tail, 2
• NCBI Official Symbol: KIR2DL2
• NCBI Official Synonym Symbols: NKAT6; p58.2; CD158b; NKAT-6; CD158B1
• NCBI Protein Information: killer cell immunoglobulin-like receptor 2DL2; p58 NK receptor CL-43; MHC class I NK cell receptor; CD158 antigen-like family member B1; natural killer-associated transcript 6; p58 natural killer cell receptor clone CL-43
• UniProt Protein Name: Killer cell immunoglobulin-like receptor 2DL2
• Protein Family: Killer cell immunoglobulin-like receptor
• UniProt Gene Name: KIR2DL2
• UniProt Synonym Gene Names: CD158B1; NKAT6; NKAT-6
• UniProt Entry Name: KI2L2_HUMAN

Uniprot Description

KIR2DL2: Receptor on natural killer (NK) cells for HLA-Cw1, 3, 7, and 8 allotypes. Inhibits the activity of NK cells thus preventing cell lysis. Belongs to the immunoglobulin superfamily.

Protein type: Membrane protein, integral; Receptor, misc.

Chromosomal Location of Human Ortholog: 19q13.4

Cellular Component: plasma membrane; integral to membrane

Biological Process: regulation of immune response