Rabbit Kip1, p27 Polyclonal Antibody
Kip1, p27 (cdk Inhibitor, Cyclin Dependent Kinase Inhibitor)
Reactivity
Human, Mouse, Rat
Applications
ELISA, Western Blot, Immunoprecipitation, Immunohistochemistry
Purity
Affinity PurifiedPurified by immunoaffinity chromatography.
Synonyms
Kip1; p27; Polyclonal Antibody; p27 (cdk Inhibitor; Cyclin Dependent Kinase Inhibitor); Anti -Kip1; anti-Kip1; p27 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Specificity
Recognizes a protein of 27kD, identified as human p27Kip1. Species Crossreactivity: Mouse and Rat.
Purity/Purification
Affinity Purified
Purified by immunoaffinity chromatography.
Purified by immunoaffinity chromatography.
Form/Format
Supplied as a liquid in 10mM PBS, pH 7.6, 0.2% BSA and 15mM sodium azide, before the addition of glycerol to 40%.
Applicable Applications for anti-Kip1, p27 antibody
ELISA (EL/EIA), Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC)
Application Notes
Suitable for use in Western Blot, Immunoprecipitation and Immunohistochemistry.
Dilution: Western Blot: 1.0ug/ml for 2hrs at RT.
Immunohistochemistry (Formalin/paraffin): 1:50 for 30 min at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.
Note: If staining is too light, use lower dilution or longer incubation time. If the staining is too strong, use higher dilution or shorter time.
Dilution: Western Blot: 1.0ug/ml for 2hrs at RT.
Immunohistochemistry (Formalin/paraffin): 1:50 for 30 min at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.
Note: If staining is too light, use lower dilution or longer incubation time. If the staining is too strong, use higher dilution or shorter time.
Immunogen
Synthetic peptide derived from C-terminal of human p27Kip1. Cellular Localization: Nuclear
Positive Control
MAD109, Ls174T. Colon carcinoma.
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage, aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Related Product Information for anti-Kip1, p27 antibody
p27Kip1 is a candidate tumor suppressor gene and functions as a negative regulator of G1 progression and has been proposed to function as a possible mediator of TGF-b induced G1 arrest.
Product Categories/Family for anti-Kip1, p27 antibody
Similar Products
Product Notes
The Kip1, p27 (Catalog #AAA630915) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Kip1, p27 (cdk Inhibitor, Cyclin Dependent Kinase Inhibitor) reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Kip1, p27 can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC). Suitable for use in Western Blot, Immunoprecipitation and Immunohistochemistry. Dilution: Western Blot: 1.0ug/ml for 2hrs at RT. Immunohistochemistry (Formalin/paraffin): 1:50 for 30 min at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min. Note: If staining is too light, use lower dilution or longer incubation time. If the staining is too strong, use higher dilution or shorter time. Researchers should empirically determine the suitability of the Kip1, p27 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Kip1, p27, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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