Immunohistochemistry (IHC)
(MBS9603306 at 1/100 staining Human breast cancer tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Rabbit Kindlin 2 Polyclonal Antibody | anti-FERMT2 antibody
Kindlin 2 Antibody
Gene Names
FERMT2; MIG2; KIND2; mig-2; UNC112; PLEKHC1; UNC112B
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
Kindlin 2; Polyclonal Antibody; Kindlin 2 Antibody; FLJ34213; FLJ44462; UNC112; FERM2_HUMAN; Fermitin family homolog 2; Fermt2; KIND2; Kindlin-2; MIG-2; MIG2; Mitogen-inducible gene 2 protein; PH domain-containing family C member 1; Pleckstrin homology domain containing family C (with FERM domain) member 1; Pleckstrin homology domain-containing family C member 1; PLEKHC1; UNC112B; anti-FERMT2 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
Kindlin 2 antibody detects endogenous levels of Kindlin 2.
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
687
Applicable Applications for anti-FERMT2 antibody
Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
WB: 1:500-1:2000
IHC: 1:50-1:200
IHC: 1:50-1:200
Immunogen
A synthesized peptide derived from human Kindlin 2.
Subcellular Location
Cytoplasm > Cell Cortex. Cytoplasm > Cytoskeleton. Cell Junction > Focal Adhesion. Within actin stress fibers at cell-ECM focal adhesion sites.
Tissue Specificity
Ubiquitous. Found in numerous tumor tissues.
Predicted Cross Reactivity
Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Similarity
Pig (92%), Zebrafish (100%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (100%), Dog (100%), Chicken (100%), Xenopus (100%)
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Immunohistochemistry (IHC)
(MBS9603306 at 1/100 staining Human breast cancer tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Immunohistochemistry (IHC)
(MBS9603306 at 1/100 staining Human breast cancer tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Western Blot (WB)
(Western blot analysis of extracts of human placenta tissue, using Kindlin 2 antibody.)
Western Blot (WB)
(Western blot analysis of extracts of human placenta tissue, using Kindlin 2 antibody.)
Related Product Information for anti-FERMT2 antibody
Function: Scaffolding protein that enhances integrin activation mediated by TLN1 and/or TLN2, but activates integrins only weakly by itself. Binds to membranes enriched in phosphoinositides. Enhances integrin-mediated cell adhesion onto the extracellular matrix and cell spreading; this requires both its ability to interact with integrins and with phospholipid membranes. Required for the assembly of focal adhesions. Participates in the connection between extracellular matrix adhesion sites and the actin cytoskeleton and also in the orchestration of actin assembly and cell shape modulation. Recruits FBLIM1 to focal adhesions. Plays a role in the TGFB1 and integrin signaling pathways. Stabilizes active CTNNB1 and plays a role in the regulation of transcription mediated by CTNNB1 and TCF7L2/TCF4 and in Wnt signaling.
Subunit Structure: Interacts with ILK (By similarity). Interacts with FBLIM1. Interacts with ITGB1 and ITGB3. Interacts with active, unphosphorylated CTNNB1. Identified in a complex with CTNNB1 and TCF7L2/TCF4. Interacts with ITGB1; the interaction is inhibited in presence of ITGB1BP1.
Similarity: The FERM domain is not correctly detected by PROSITE or Pfam techniques because it contains the insertion of a PH domain.The PH domain binds phospholipids. Binds preferentially phosphatidylinositol-3, 4, 5-trisphosphate, and has lower affinity for phosphatidylinositol-4, 5-bisphosphate (PubMed:22030399).The N-terminal region displays a ubiquitin-type fold and mediates interaction with membranes containing negatively charged phosphatidylinositol phosphate via a surface enriched in positively charged residues. Belongs to the kindlin family.
Subunit Structure: Interacts with ILK (By similarity). Interacts with FBLIM1. Interacts with ITGB1 and ITGB3. Interacts with active, unphosphorylated CTNNB1. Identified in a complex with CTNNB1 and TCF7L2/TCF4. Interacts with ITGB1; the interaction is inhibited in presence of ITGB1BP1.
Similarity: The FERM domain is not correctly detected by PROSITE or Pfam techniques because it contains the insertion of a PH domain.The PH domain binds phospholipids. Binds preferentially phosphatidylinositol-3, 4, 5-trisphosphate, and has lower affinity for phosphatidylinositol-4, 5-bisphosphate (PubMed:22030399).The N-terminal region displays a ubiquitin-type fold and mediates interaction with membranes containing negatively charged phosphatidylinositol phosphate via a surface enriched in positively charged residues. Belongs to the kindlin family.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Observed: 78 kDa
Predicted: 78 kDa
Predicted: 78 kDa
NCBI Official Full Name
fermitin family homolog 2 isoform 2
NCBI Official Synonym Full Names
fermitin family member 2
NCBI Official Symbol
FERMT2
NCBI Official Synonym Symbols
MIG2; KIND2; mig-2; UNC112; PLEKHC1; UNC112B
NCBI Protein Information
fermitin family homolog 2
UniProt Protein Name
Fermitin family homolog 2
Protein Family
UniProt Gene Name
FERMT2
UniProt Synonym Gene Names
KIND2; MIG2; PLEKHC1; MIG-2; PH domain-containing family C member 1
Uniprot Description
Scaffolding protein that enhances integrin activation mediated by TLN1 and/or TLN2, but activates integrins only weakly by itself. Binds to membranes enriched in phosphoinositides. Enhances integrin-mediated cell adhesion onto the extracellular matrix and cell spreading; this requires both its ability to interact with integrins and with phospholipid membranes. Required for the assembly of focal adhesions. Participates in the connection between extracellular matrix adhesion sites and the actin cytoskeleton and also in the orchestration of actin assembly and cell shape modulation. Recruits FBLIM1 to focal adhesions. Plays a role in the TGFB1 and integrin signaling pathways. Stabilizes active CTNNB1 and plays a role in the regulation of transcription mediated by CTNNB1 and TCF7L2/TCF4 and in Wnt signaling.
Research Articles on FERMT2
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Product Notes
The FERMT2 fermt2 (Catalog #AAA9603306) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Kindlin 2 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Kindlin 2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC). WB: 1:500-1:2000 IHC: 1:50-1:200. Researchers should empirically determine the suitability of the FERMT2 fermt2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Kindlin 2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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