Mouse anti-Human CD7 Monoclonal Antibody | anti-CD7 antibody

anti-human CD7-FITC

Cat. Num. AAA666653

• Gene Names: CD7; GP40; TP41; Tp40; LEU-9
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay
• Synonyms: CD7; Monoclonal Antibody; anti-human CD7-FITC; Monoclonal anti-human CD7/FITC; anti-CD7 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG2b
• Clone Number: 3A1e
• Form/Format: 50 mM Sodium Phosphate pH 7.5, 100 mM Potassium Chloride, 150 mM NaCl, 5% Glycerol, 0.2% BSA, 0.04% NaN₃ (as a preservative).
• Sequence Length: 240

• Applicable Applications for anti-CD7 antibody: Flow Cytometry (FC/FACS)
• Application Notes: Working Dilution: 1:50 (or use 1.6ul of concentrated stock per 5 x 10⁵-cell test.)
• Immunogen: Human HSB2-T cells
• Production: Protein A purified antibody from tissue culture supernatant was reacted with FITC. Unconjugated FITC was separated from antibody/FITC conjugate by desalting column. The antibody/FITC conjugate is at 0.5 mg/ml with a Fluorescein/Antibody molar ratio of 4.6.
• Performance: Five x 10⁵ ficoll prepared peripheral blood mononuclear cells (PBMC) were washed and incubated 45 minutes on ice with 80 ul of anti-CD7/FITC at a 1:50 dilution(10ug/ml). Cells were washed three times, fixed and analyzed by FACS. A 67% sub population of the cells stained positive with a mean shift of 0.95 log₁₀ fluorescent units when compared to a Mouse IgG2b/FITC negative control (MBS666481) at a similar concentration. Binding was blocked when cells were pre incubated 10 minutes with 20 ul of 0.5 mg/ml anti-CD7 antibody (MBS666367).
• Preparation and Storage: Store at 2-5°C. Freeze/Thawing is not recommended. Protect from light.; Product should retain activity for at least 12 months after shipping date when stored as recommended.

Flow Cytometry (FC/FACS)

Related Product Information for anti-CD7 antibody

Human CD7 is one of the earliest T lineage markers and is found on most thymocytes and peripheral blood T cells. The function of CD7 is not yet fully known, although cross-linking of CD7 with antibodies induces a T cell transmembrane calcium flux and CD7 expression is induced by ionomycin. Antibody 3Ale recognizes a CD7 molecule of 40 kd. Antibody 3Ale induces T cell transmembrane calcium flux.

References

R.E. Ware, et al, (1989) J Immunol 143: 3632-3640. R.E. Ware, et al, (1991) J Immunol 147: 2787-2794.

NCBI and Uniprot Product Information

• NCBI GI #: 180164
• NCBI GeneID: 924
• NCBI Official Full Name: CD7 antigen protein
• NCBI Official Synonym Full Names: CD7 molecule
• NCBI Official Symbol: CD7
• NCBI Official Synonym Symbols: GP40; TP41; Tp40; LEU-9
• NCBI Protein Information: T-cell antigen CD7; p41 protein; CD7 antigen (p41); T-cell leukemia antigen; T-cell surface antigen Leu-9
• UniProt Protein Name: T-cell antigen CD7
• Protein Family: CD70 antigen
• UniProt Gene Name: CD7
• UniProt Entry Name: CD7_HUMAN

NCBI Description

This gene encodes a transmembrane protein which is a member of the immunoglobulin superfamily. This protein is found on thymocytes and mature T cells. It plays an essential role in T-cell interactions and also in T-cell/B-cell interaction during early lymphoid development. [provided by RefSeq, Jul 2008]

Uniprot Description

CD7: Not yet known.

Protein type: Membrane protein, integral; Immunoglobulin superfamily

Chromosomal Location of Human Ortholog: 17q25.2-q25.3

Cellular Component: membrane; plasma membrane; integral to membrane

Molecular Function: receptor activity

Biological Process: T cell activation; homeostasis of number of cells within a tissue; immune response; transmembrane receptor protein tyrosine kinase signaling pathway