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Western Blot (WB) (Western Blot: Sample: Recombinant protein.)

Rabbit anti-Mouse Killer Cell Lectin Like Receptor Subfamily K Polyclonal Antibody | anti-KLRK1 antibody

Killer Cell Lectin Like Receptor Subfamily K, Member 1 (KLRK1) Polyclonal Antibody

Gene Names
Klrk1; Nkg2d; NKG2-D; D6H12S2489E
Reactivity
Mouse
Applications
Western Blot, Immunocytochemistry, Immunohistochemistry, ELISA
Purity
Affinity Chromatography
Synonyms
Killer Cell Lectin Like Receptor Subfamily K; Polyclonal Antibody; Member 1 (KLRK1) Polyclonal Antibody; CD314; KLR-K1; KLR; NKG2-D; NKG2-D Type II Integral Membrane Protein; NK cell receptor D; NKG2-D-activating NK receptor; anti-KLRK1 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Mouse
Clonality
Polyclonal
Specificity
The antibody is a rabbit polyclonal antibody raised against KLRK1. It has been selected for its ability to recognize KLRK1 in immunohistochemical staining and western blotting.
Purity/Purification
Affinity Chromatography
Form/Format
Supplied as solution form in PBS, pH7.4 containing 0.02% NaN3, 50% glycerol.
Concentration
500ug/ml (varies by lot)
Sequence Length
216
Applicable Applications for anti-KLRK1 antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA)
Application Notes
Western Blot: 1:50-400
Immunohistochemistry in formalin fixed frozen section: 1:50-500
Enzyme-linked Immunosorbent Assay: 1:100-200
Immunoglobulin Type
IgG
Conjugate
No Conjugate
Cross Reactivity
Mouse
Organism Species
Mouse
Fragment
KLRK1 (Phe90~Val232)
Quality Control
Content: The quality control contains recombinant KLRK1 (Phe90~Val232) disposed in loading buffer.
Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate.
5uL per well when used in enhanced chemilumescent (ECL).
Note: The quality control is specifically manufactured as the positive control.Not used for other purposes.
Loading Buffer: 100mM Tris(pH8.8), 2% SDS, 200mM NaCl, 50% glycerol,BPB 0.01%, NaN3 0.02%.
Conjugated Antibody
The APC conjugated antibody version of this item is also available as catalog #MBS2058829
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Western Blot: Sample: Recombinant protein.)

Western Blot (WB) (Western Blot: Sample: Recombinant protein.)

Immunohistochemistry (IHC)

(DAB staining on fromalin fixed paraffin- embedded Kidney tissue))

Immunohistochemistry (IHC) (DAB staining on fromalin fixed paraffin- embedded Kidney tissue))

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
25,148 Da
NCBI Official Full Name
NKG2-D type II integral membrane protein isoform b
NCBI Official Synonym Full Names
killer cell lectin-like receptor subfamily K, member 1
NCBI Official Symbol
Klrk1
NCBI Official Synonym Symbols
Nkg2d; NKG2-D; D6H12S2489E
NCBI Protein Information
NKG2-D type II integral membrane protein
UniProt Protein Name
NKG2-D type II integral membrane protein
UniProt Gene Name
Klrk1
UniProt Synonym Gene Names
Nkg2d

Uniprot Description

KLRK1: Receptor for MICA, MICB, ULBP1, ULBP2, ULBP3 (ULBP2>ULBP1>ULBP3) and ULBP4. Plays a role as a receptor for the recognition of MHC class I HLA-E molecules by NK cells and some cytotoxic T-cells. Involved in the immune surveillance exerted by T- and B-lymphocytes. 1 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell surface; Membrane protein, integral; Receptor, misc.

Chromosomal Location of Human Ortholog: 6 F3|6 63.44 cM

Cellular Component: cell surface; external side of plasma membrane; integral component of membrane; membrane; plasma membrane

Molecular Function: carbohydrate binding; kinase binding; MHC class I protein binding; MHC class Ib receptor activity; protein binding; protein homodimerization activity

Biological Process: adaptive immune response; cell differentiation; defense response to Gram-positive bacterium; immune system process; innate immune response; natural killer cell activation; positive regulation of apoptosis; positive regulation of interferon-gamma production; positive regulation of myeloid dendritic cell activation; positive regulation of natural killer cell mediated cytotoxicity; positive regulation of natural killer cell mediated cytotoxicity directed against tumor cell target; positive regulation of nitric oxide biosynthetic process; stimulatory C-type lectin receptor signaling pathway

Research Articles on KLRK1

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Product Notes

The KLRK1 klrk1 (Catalog #AAA2032644) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Killer Cell Lectin Like Receptor Subfamily K, Member 1 (KLRK1) Polyclonal Antibody reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Killer Cell Lectin Like Receptor Subfamily K can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA). Western Blot: 1:50-400 Immunohistochemistry in formalin fixed frozen section: 1:50-500 Enzyme-linked Immunosorbent Assay: 1:100-200. Researchers should empirically determine the suitability of the KLRK1 klrk1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Killer Cell Lectin Like Receptor Subfamily K, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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