Western Blot (WB)
(Western blot analysis of extracts of mouse kidney tissue, using KIFC3 antibody.)
Rabbit KIFC3 Polyclonal Antibody | anti-KIFC3 antibody
KIFC3 Antibody
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
KIFC3; Polyclonal Antibody; KIFC3 Antibody; DKFZp686D23201; FLJ34694; Kifc3; KIFC3_HUMAN; Kinesin family member C3; Kinesin-like protein KIFC3; anti-KIFC3 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
KIFC3 antibody detects endogenous levels of KIFC3.
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
687
Applicable Applications for anti-KIFC3 antibody
Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
WB: 1:500-1:2000
IHC: 1:50-1:200
IHC: 1:50-1:200
Immunogen
A synthesized peptide derived from human KIFC3.
Subcellular Location
Cell Junction > Adherens Junction. Cytoplasm > Cytoskeleton > Centrosome. Cytoplasmic Vesicle Membrane. Apical Cell Membrane. On membrane organelles immediately beneath the apical plasma membrane of renal tubular epithelial cells. Localized in the distal tubules and loops of Henle in the kidney, but not in the proximal tubules or the glomeruli, with stronger staining in the apical area of these epithelial cells (By similarity). Localizes along zonula adherens only at mature cell-cell contacts.
Predicted Cross Reactivity
Pig, Bovine, Horse, Sheep, Rabbit, Dog
Similarity
Pig (92%), Bovine (92%), Horse (83%), Sheep (92%), Rabbit (83%), Dog (92%)
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Western Blot (WB)
(Western blot analysis of extracts of mouse kidney tissue, using KIFC3 antibody.)
Western Blot (WB)
(Western blot analysis of extracts of mouse kidney tissue, using KIFC3 antibody.)
Immunohistochemistry (IHC)
(MBS9603515 at 1/100 staining Human lymph cancer tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Immunohistochemistry (IHC)
(MBS9603515 at 1/100 staining Human lymph cancer tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Related Product Information for anti-KIFC3 antibody
Function: Minus-end microtubule-dependent motor protein. Involved in apically targeted transport (By similarity). Required for zonula adherens maintenance.
Similarity: Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family.
Similarity: Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Observed: 80-85 kDa; 95-100 kDa
Predicted: 93 kDa
Predicted: 93 kDa
NCBI Official Full Name
kinesin-like protein KIFC3 isoform 3
NCBI Official Synonym Full Names
kinesin family member C3
NCBI Official Symbol
KIFC3
NCBI Protein Information
kinesin-like protein KIFC3
UniProt Protein Name
Kinesin-like protein KIFC3
Protein Family
UniProt Gene Name
KIFC3
NCBI Description
This gene encodes a member of the kinesin-14 family of microtubule motors. Members of this family play a role in the formation, maintenance and remodeling of the bipolar mitotic spindle. The protein encoded by this gene has cytoplasmic functions in the interphase cells. It may also be involved in the final stages of cytokinesis. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jan 2016]
Uniprot Description
Minus-end microtubule-dependent motor protein. Involved in apically targeted transport (). Required for zonula adherens maintenance.
Research Articles on KIFC3
Similar Products
Product Notes
The KIFC3 kifc3 (Catalog #AAA9603515) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The KIFC3 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's KIFC3 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC). WB: 1:500-1:2000 IHC: 1:50-1:200. Researchers should empirically determine the suitability of the KIFC3 kifc3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "KIFC3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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