Rabbit anti-Human KIF20A Polyclonal Antibody | anti-KIF20A antibody

KIF20A Antibody

Cat. Num. AAA9401413

• Gene Names: KIF20A; MKLP2; RAB6KIFL
• Reactivity: Human
• Applications: Western Blot, Immunohistochemistry
• Purity: Antigen affinity purification.
• Synonyms: KIF20A; Polyclonal Antibody; KIF20A Antibody; MKLP2; RAB6KIFL; anti-KIF20A antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Specificity: The antibody detects endogenous levels of total KIF20A protein.
• Purity/Purification: Antigen affinity purification.
• Form/Format: Rabbit IgG in pH7.3 PBS, 0.05% NaN3, 50% Glycerol.
• Concentration: 1.2 mg/ml (varies by lot)
• Sequence Length: 890

• Applicable Applications for anti-KIF20A antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Western blotting: 1:200-1:1000; Immunohistochemistry: 1:50-1:200
• Immunogen Type: Recombinant Protein
• Immunogen Description: Fusion protein corresponding to residues near the C terminal of human kinesin family member 20A
• Target Name: KIF20A
• Preparation and Storage: Store at -20 degree C

Testing Data

(Gel: 6%SDS-PAGE Lysates (from left to right): K562, 293T and hela cell Amount of lysate: 40ug per lane Primary antibody: 1/400 dilution Secondary antibody dilution: 1/8000 Exposure time: 10 seconds)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded Human thyroid cancer tissue using at dilution 1/20.)

Related Product Information for anti-KIF20A antibody

KIF20A (kinesin family member 20A), also known as Rabkinesin-6, RAB6KIFL (Rab6-interacting kinesin-like protein), GG10_2 or MKLP2 (mitotic kinesin-like protein 2), is a 890 amino acid protein that contains one kinesin-motor domain and belongs to the Kinesin-like protein family. KIF20A locates to the Golgi apparatus and interacts with guanosine triphosphate (GTP)-bound forms of RAB 6. KIF20A may be responsible for the retrograde RAB 6 regulated transport of Golgi membranes and related vesicles along microtubules.

Product Categories/Family for anti-KIF20A antibody

Total protein Ab

NCBI and Uniprot Product Information

• NCBI GI #: 5032013
• NCBI GeneID: 10112
• NCBI Accession #: NP_005724.1
• NCBI GenBank Nucleotide #: NM_005733.2
• UniProt Accession #: O95235; B4DL79; D3DQB6
• Molecular Weight: 98,100 Da
• NCBI Official Full Name: kinesin-like protein KIF20A
• NCBI Official Synonym Full Names: kinesin family member 20A
• NCBI Official Symbol: KIF20A
• NCBI Official Synonym Symbols: MKLP2; RAB6KIFL
• NCBI Protein Information: kinesin-like protein KIF20A
• UniProt Protein Name: Kinesin-like protein KIF20A
• Protein Family: Kinesin-like protein
• UniProt Gene Name: KIF20A
• UniProt Synonym Gene Names: MKLP2; RAB6KIFL; MKlp2
• UniProt Entry Name: KI20A_HUMAN

Uniprot Description

KIF20A: Mitotic kinesin required for chromosome passenger complex (CPC)-mediated cytokinesis. Following phosphorylation by PLK1, involved in recruitment of PLK1 to the central spindle. Interacts with guanosine triphosphate (GTP)-bound forms of RAB6A and RAB6B. May act as a motor required for the retrograde RAB6 regulated transport of Golgi membranes and associated vesicles along microtubules. Has a microtubule plus end-directed motility. Belongs to the kinesin-like protein family.

Protein type: Microtubule-binding; Motor

Chromosomal Location of Human Ortholog: 5q31

Cellular Component: nucleoplasm; Golgi apparatus; microtubule; kinesin complex; spindle; midbody

Molecular Function: protein binding; transporter activity; microtubule binding; ATPase activity; microtubule motor activity; protein kinase binding; ATP binding

Biological Process: vesicle-mediated transport; protein transport; cytokinesis after mitosis; cell separation during cytokinesis; metabolic process; cytokinesis; mitotic cell cycle; microtubule-based movement; microtubule bundle formation