Rabbit AKR1B1 Polyclonal Antibody | anti-AKR1B1 antibody

Anti-AKR1B1 Antibody

Cat. Num. AAA178791

• Gene Names: AKR1B1; AR; ADR; ALR2; ALDR1
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry
• Purity: Immunogen affinity purified.
• Synonyms: AKR1B1; Polyclonal Antibody; Anti-AKR1B1 Antibody; ADR; AKR1B 1; Akr1b1; Aldehyde reductase 1; Aldehyde reductase; Aldose reductase; aldr 1; aldr1; ALR2; AR; P15121; aldo-keto reductase family 1 member B; anti-AKR1B1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Immunogen affinity purified.
• Form/Format: Lyophilized; Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
• Sequence Length: 316

• Applicable Applications for anti-AKR1B1 antibody: Western Blot (WB), Immunohistochemistry (IHC) Paraffin
• Application Notes: Western Blot: 0.1-0.5ug/ml; Immunohistochemistry(IHC) Paraffin: 0.5-1ug/ml
• Notes: Tested Species: In-house tested species with positive results.; By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections.; Other applications have not been tested.
• Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Immunogen: E. coli-derived human AKR1B1 recombinant protein (Position: L228-F316). Human AKR1B1 shares 87.5% amino acid (aa) sequence identity with both mouse and rat AKR1B1.
• Preparation and Storage: Store at -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.

Western Blot (WB)

(Western blot analysis of AKR1B1 expression in rat cardiac muscle extract (lane 1), human placenta extract (lane 2) and HELA whole cell lysates (lane 3). AKR1B1 at 36KD was detected using rabbit anti- AKR1B1 Antigen Affinity purified polyclonal antibody at 0.5ug/mL. The blot was developed using chemiluminescence (ECL) method. )

Immunohistochemistry (IHC)

(AKR1B1 was detected in paraffin-embedded sections of rat intestine tissues using rabbit anti- AKR1B1 Antigen Affinity purified polyclonal antibody at 1ug/mL. The immunohistochemical section was developed using SABC method. )

Immunohistochemistry (IHC)

(AKR1B1 was detected in paraffin-embedded sections of rat adrenal gland tissues using rabbit anti- AKR1B1 Antigen Affinity purified polyclonal antibody at 1ug/mL. The immunohistochemical section was developed using SABC method. )

Immunohistochemistry (IHC)

(AKR1B1 was detected in paraffin-embedded sections of human placenta tissues using rabbit anti- AKR1B1 Antigen Affinity purified polyclonal antibody at 1 ug/mL. The immunohistochemical section was developed using SABC method. )

Related Product Information for anti-AKR1B1 antibody

Rabbit IgG polyclonal antibody for Aldose reductase(AKR1B1) detection.
Background: Aldo-keto reductase family 1, member B1 (aldose reductase), also known as AR, is an enzyme that in humans is encoded by the AKR1B1 gene. This gene encodes a member of the aldo/keto reductase superfamily, which consists of more than 40 known enzymes and proteins. This member catalyzes the reduction of a number ofaldehydes, including the aldehyde form of glucose, and is thereby implicated in the development of diabetic complications by catalyzing the reduction of glucose to sorbitol.

References

1. Ferraretto A, Negri A, Giuliani A, et al. (1993). "Aldose reductase is involved in long-term adaptation of EUE cells to hyperosmotic stress".Biochim. Biophys. Acta 1175(3): 283-8.
2. Graham A, Heath P, Morten JE, Markham AF (March 1991). "The human aldose reductase gene maps to chromosome region 7q35". Hum. Genet. 86 (5): 509-14.

NCBI and Uniprot Product Information

• NCBI GI #: 4502049
• NCBI GeneID: 231
• NCBI Accession #: NP_001619.1
• NCBI GenBank Nucleotide #: NM_001628.3
• UniProt Accession #: P15121; Q5U031; Q6FGA4; Q6ICP2; Q9BS21; Q9UCI9; B2R8N3
• Molecular Weight: 35,853 Da
• NCBI Official Full Name: aldose reductase isoform 1
• NCBI Official Synonym Full Names: aldo-keto reductase family 1 member B
• NCBI Official Symbol: AKR1B1
• NCBI Official Synonym Symbols: AR; ADR; ALR2; ALDR1
• NCBI Protein Information: aldose reductase
• UniProt Protein Name: Aldose reductase
• Protein Family: Aldose reductase
• UniProt Gene Name: AKR1B1
• UniProt Synonym Gene Names: ALDR1; AR

NCBI Description

This gene encodes a member of the aldo/keto reductase superfamily, which consists of more than 40 known enzymes and proteins. This member catalyzes the reduction of a number of aldehydes, including the aldehyde form of glucose, and is thereby implicated in the development of diabetic complications by catalyzing the reduction of glucose to sorbitol. Multiple pseudogenes have been identified for this gene. The nomenclature system used by the HUGO Gene Nomenclature Committee to define human aldo-keto reductase family members is known to differ from that used by the Mouse Genome Informatics database. [provided by RefSeq, Feb 2009]

Uniprot Description

AKR1B1: Catalyzes the NADPH-dependent reduction of a wide variety of carbonyl-containing compounds to their corresponding alcohols with a broad range of catalytic efficiencies. Belongs to the aldo/keto reductase family.

Protein type: Carbohydrate Metabolism - fructose and mannose; Carbohydrate Metabolism - galactose; Carbohydrate Metabolism - pentose and glucuronate interconversions; Carbohydrate Metabolism - pyruvate; EC 1.1.1.21; Lipid Metabolism - glycerolipid; Oxidoreductase

Chromosomal Location of Human Ortholog: 7q33

Cellular Component: cytoplasm; cytosol; nucleoplasm

Molecular Function: aldehyde reductase activity; aldo-keto reductase activity; electron carrier activity; glyceraldehyde oxidoreductase activity

Biological Process: C21-steroid hormone biosynthetic process; carbohydrate metabolic process; fructose biosynthetic process; response to stress