Western Blot (WB)
(Western Blot analysis of KDEL on different lysates using anti-KDEL antibody at 1/1,000 dilution. Positive control: Lane 1: Rat testis tissueLane 2: Human placenta tissueLane 3: Mouse testis tissueLane 4: 293)
Rabbit KDEL Polyclonal Antibody | anti-KDEL antibody
KDEL Polyclonal Antibody
ICC: 1:400-1:800
IHC: 1:100-1:400
FC/FACS: 1:50-1:100
Aliquot store at -20 degree C or -80 degree C.
Avoid repeated freeze/thaw cycles.
Western Blot (WB)
(Western Blot analysis of KDEL on different lysates using anti-KDEL antibody at 1/1,000 dilution. Positive control: Lane 1: Rat testis tissueLane 2: Human placenta tissueLane 3: Mouse testis tissueLane 4: 293)
Western Blot (WB)
(Western Blot analysis of KDEL on different lysates using anti-KDEL antibody at 1/1,000 dilution. Positive control: Lane 1: Rat testis tissueLane 2: Human placenta tissueLane 3: Mouse testis tissueLane 4: 293)
Immunocytochemistry (ICC)
(ICC staining KDEL in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
Immunocytochemistry (ICC)
(ICC staining KDEL in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
NCBI and Uniprot Product Information
NCBI Description
Retention of resident soluble proteins in the lumen of the endoplasmic reticulum (ER) is achieved in both yeast and animal cells by their continual retrieval from the cis-Golgi, or a pre-Golgi compartment. Sorting of these proteins is dependent on a C-terminal tetrapeptide signal, usually lys-asp-glu-leu (KDEL) in animal cells, and his-asp-glu-leu (HDEL) in S. cerevisiae. This process is mediated by a receptor that recognizes, and binds the tetrapeptide-containing protein, and returns it to the ER. In yeast, the sorting receptor encoded by a single gene, ERD2, which is a seven-transmembrane protein. Unlike yeast, several human homologs of the ERD2 gene, constituting the KDEL receptor gene family, have been described. The protein encoded by this gene was the first member of the family to be identified, and it encodes a protein structurally and functionally similar to the yeast ERD2 gene product. [provided by RefSeq, Jul 2008]
Uniprot Description
KDELR1: Required for the retention of luminal endoplasmic reticulum proteins. Determines the specificity of the luminal ER protein retention system. Also required for normal vesicular traffic through the Golgi. This receptor recognizes K-D-E-L. Belongs to the ERD2 family.
Protein type: Endoplasmic reticulum; Membrane protein, multi-pass; Membrane protein, integral
Chromosomal Location of Human Ortholog: 19q13.3
Cellular Component: COPI coated vesicle membrane; endoplasmic reticulum membrane; ER-Golgi intermediate compartment membrane; cis-Golgi network; membrane; ER-Golgi intermediate compartment; integral to membrane
Molecular Function: KDEL sequence binding
Biological Process: vesicle-mediated transport; intracellular protein transport; protein retention in ER
Research Articles on KDEL
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Product Notes
The KDEL kdelr1 (Catalog #AAA3012948) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The KDEL Polyclonal Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's KDEL can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS). WB: 1:500-1:2000 ICC: 1:400-1:800 IHC: 1:100-1:400 FC/FACS: 1:50-1:100. Researchers should empirically determine the suitability of the KDEL kdelr1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "KDEL, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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