Rabbit anti-Human, Mouse CDK3 Polyclonal Antibody | anti-CDK3 antibody

CDK3 (Cyclin-dependent Kinase 3, Cell Division Protein Kinase 3, CDKN3) (PE)

Cat. Num. AAA6373531

• Reactivity: Human, Mouse
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: CDK3; Polyclonal Antibody; CDK3 (Cyclin-dependent Kinase 3; Cell Division Protein Kinase 3; CDKN3) (PE); anti-CDK3 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human CDK3. Species Crossreactivity: mouse.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-CDK3 antibody: Western Blot (WB)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: Full length human CDK3, aa1-305 (NP_001249.1).
• Immunogen Sequence: MDMFQKVEKIGEGTYGVVYKAKNRETGQLVALKKIRLDLEMEGVPSTAIREISLLKELKHPNIVRLLDVVHNERKLYLVFEFLSQDLKKYMDSTPGSELPLHLIKSYLFQLLQGVSFCHSHRVIHRDLKPQNLLINELGAIKLADFGLARAFGVPLRTYTHEVVTLWYRAPEILLGSKFYTTAVDIWSIGCIFAEMVTRKALFPGDSEIDQLFRIFRMLGTPSEDTWPGVTQLPDYKGSFPKWTRKGLEEIVPNLEPEGRDLLMQLLQYDPSQRITAKTALAHPYFSSPEPSPAARQYVLQRFRH
• Conjugate: PE
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(CDK3 rabbit polyclonal antibody. Western Blot analysis of CDK3 expression in mouse brain.)

Western Blot (WB)

(Western Blot analysis of CDK3 expression in transfected 293T cell line by CDK3 polyclonal antibody. Lane 1: CDK3 transfected lysate (35kD). Lane 2: Non-transfected lysate.)

Related Product Information for anti-CDK3 antibody

Serine/threonine-protein kinase that plays a critical role in the control of the eukaryotic cell cycle; involved in G0-G1 and G1-S cell cycle transitions. Interacts with CCNC/cyclin-C during interphase. Phosphorylates histone H1, ATF1, RB1 and CABLES1. ATF1 phosphorylation triggers ATF1 transactivation and transcriptional activities, and promotes cell proliferation and transformation. CDK3/cyclin-C mediated RB1 phosphorylation is required for G0-G1 transition. Promotes G1-S transition probably by contributing to the activation of E2F1, E2F2 and E2F3 in a RB1-independent manner.

Product Categories/Family for anti-CDK3 antibody

Antibodies; Protein Kinases

NCBI and Uniprot Product Information

• NCBI GI #: 4557439
• NCBI GeneID: 1018
• NCBI Accession #: NP_001249.1
• NCBI GenBank Nucleotide #: NM_001258.1
• UniProt Accession #: Q00526
• Molecular Weight: 35,046 Da
• NCBI Official Full Name: cyclin-dependent kinase 3
• NCBI Official Synonym Full Names: cyclin-dependent kinase 3
• NCBI Official Symbol: CDK3
• NCBI Protein Information: cyclin-dependent kinase 3; cell division protein kinase 3
• UniProt Protein Name: Cyclin-dependent kinase 3
• Protein Family: Cyclin-dependent kinase
• UniProt Gene Name: CDK3
• UniProt Synonym Gene Names: CDKN3
• UniProt Entry Name: CDK3_HUMAN

NCBI Description

This gene encodes a member of the cyclin-dependent protein kinase family. The protein promotes entry into S phase, in part by activating members of the E2F family of transcription factors. The protein also associates with cyclin C and phosphorylates the retinoblastoma 1 protein to promote exit from G0. [provided by RefSeq, Jul 2008]

Uniprot Description

CDK3: a protein kinase of the CDK family. May be involved in cell cycle control. Can phosphorylate histone H1.

Protein type: Protein kinase, CMGC; Cell cycle regulation; EC 2.7.11.22; Protein kinase, Ser/Thr (non-receptor); Kinase, protein; CMGC group; CDK family; CDK/CDK1 subfamily; CDK1 subfamily

Chromosomal Location of Human Ortholog: 17q25.1

Molecular Function: cyclin-dependent protein kinase activity; ATP binding

Biological Process: mitosis; cell proliferation; G0 to G1 transition; regulation of cell cycle; cell division; protein amino acid phosphorylation; G1/S transition of mitotic cell cycle