Rabbit KDEL 2 Polyclonal Antibody | anti-KDEL2 antibody

KDEL Receptor 2 (L119) Polyclonal Antibody

Cat. Num. AAA3003442

• Gene Names: KDELR2; ELP-1; ERD2.2
• Reactivity: Human, Mouse, Rat
• Applications: Immunohistochemistry, Immunofluorescence
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: KDEL 2; Polyclonal Antibody; KDEL Receptor 2 (L119) Polyclonal Antibody; ER lumen protein retaining receptor 2; ERD2-like protein 1; ELP-1; ELP1; KDEL endoplasmic reticulum protein retention receptor 2; KDEL receptor 2; KDELR2; ERD2.2; anti-KDEL2 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: KDEL Receptor 2 (L119) polyclonal antibody detects endogenous levels of KDEL Receptor 2 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 186

• Applicable Applications for anti-KDEL2 antibody: Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: IHC: 1:50-1:200; IF: 1:50-1:200
• Immunogen: Synthetic peptide, corresponding to amino acids 100-150 of Human KDEL Receptor 2.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Immunohistochemistry (IHC)

(Immunohistochemistry (IHC) analyzes of KDEL Receptor 2 (L119) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.)

Related Product Information for anti-KDEL2 antibody

Retention of resident soluble proteins in the lumen of the endoplasmic reticulum (ER) is achieved in both yeast and animal cells by their continual retrieval from the cis-Golgi, or a pre-Golgi compartment. Sorting of these proteins is dependent on a C-terminal tetrapeptide signal, usually lys-asp-glu-leu (KDEL) in animal cells, and his-asp-glu-leu (HDEL) in S. cerevisiae. This process is mediated by a receptor that recognizes, and binds the tetrapeptide-containing protein, and returns it to the ER. In yeast, the sorting receptor encoded by a single gene, ERD2, which is a seven-transmembrane protein. Unlike yeast, several human homologs of the ERD2 gene, constituting the KDEL receptor gene family, have been described. The protein encoded by this gene was the first member of the family to be identified, and it encodes a protein structurally and functionally similar to the yeast ERD2 gene product.

NCBI and Uniprot Product Information

• NCBI GI #: 154736713
• NCBI GeneID: 11014
• NCBI Accession #: NP_001094073.1
• NCBI GenBank Nucleotide #: NP_001094073.1
• UniProt Accession #: P33947
• Molecular Weight: ~ 24kDa
• NCBI Official Full Name: ER lumen protein-retaining receptor 2 isoform 2
• NCBI Official Synonym Full Names: KDEL endoplasmic reticulum protein retention receptor 2
• NCBI Official Symbol: KDELR2
• NCBI Official Synonym Symbols: ELP-1; ERD2.2
• NCBI Protein Information: ER lumen protein-retaining receptor 2
• UniProt Protein Name: ER lumen protein retaining receptor 2
• UniProt Gene Name: KDELR2
• UniProt Synonym Gene Names: ERD2.2; ELP-1
• UniProt Entry Name: ERD22_HUMAN

NCBI Description

Retention of resident soluble proteins in the lumen of the endoplasmic reticulum (ER) is achieved in both yeast and animal cells by their continual retrieval from the cis-Golgi, or a pre-Golgi compartment. Sorting of these proteins is dependent on a C-terminal tetrapeptide signal, usually lys-asp-glu-leu (KDEL) in animal cells, and his-asp-glu-leu (HDEL) in S. cerevisiae. This process is mediated by a receptor that recognizes, and binds the tetrapeptide-containing protein, and returns it to the ER. In yeast, the sorting receptor encoded by a single gene, ERD2, is a seven-transmembrane protein. Unlike yeast, several human homologs of the ERD2 gene, constituting the KDEL receptor gene family, have been described. KDELR2 was the second member of the family to be identified, and it encodes a protein which is 83% identical to the KDELR1 gene product. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Jul 2008]

Uniprot Description

KDELR2: Required for the retention of luminal endoplasmic reticulum proteins. Determines the specificity of the luminal ER protein retention system. Also required for normal vesicular traffic through the Golgi. This receptor recognizes K-D-E-L. Belongs to the ERD2 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 7p22.1

Cellular Component: Golgi apparatus; endoplasmic reticulum membrane; cis-Golgi network; endoplasmic reticulum; integral to membrane

Molecular Function: KDEL sequence binding

Biological Process: vesicle-mediated transport; intracellular protein transport; protein retention in ER