Mouse anti-Human PBX3 Monoclonal Antibody | anti-PBX3 antibody

Mouse Monoclonal [clone 1A11] (IgG2a,k) to Human PBX3

Cat. Num. AAA245763

• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot, ELISA
• Purity: Protein A Purified
• Synonyms: PBX3; Monoclonal Antibody; Mouse Monoclonal [clone 1A11] (IgG2a; k) to Human PBX3; Anti-PBX3 Antibody (clone 1A11) IHC-plus; Pre-B-cell leukemia homeobox 3; Homeobox protein PBX3; Human PBX3; anti-PBX3 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG2a,k
• Clone Number: 1A11
• Specificity: Human Pbx3
• Purity/Purification: Protein A Purified
• Form/Format: PBS, pH 7.2. Sourced in Ascites.
• Concentration: 0.5 mg/ml (varies by lot)
• Sequence Length: 434

• Applicable Applications for anti-PBX3 antibody: Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA)
• Application Notes: ELISA, IHC-P (5 ug/ml), WB; Usage: Immunohistochemistry: Formalin-fixed paraffin-embedded sections. Sandwich ELISA: Recombinant protein. Western Blot: Cell lysate, Recombinant protein.
• Target Species: Human
• Immunogen Description: PBX3 (NP_006186, 342 a.a. ~ 435 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
• Immunogen: PBX3 antibody was raised against pBX3 (NP_006186, 342 a.a. ~ 435 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
• Preparation and Storage: Store at -20 degree C or lower. Aliquot to avoid repeated freezing and thawing.

Immunohistochemistry (IHC)

(Anti-PBX3 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

Western Blot (WB)

(Western blot of PBX3 expression in K-562.)

ELISA (EIA)

(Detection limit for recombinant GST tagged PBX3 is approximately 1 ng/ml as a capture antibody.)

NCBI and Uniprot Product Information

• NCBI GI #: 5453852
• NCBI GeneID: 5090
• NCBI Accession #: NP_006186.1
• NCBI GenBank Nucleotide #: NM_006195.5
• UniProt Accession #: P40426; Q5JSA0; Q5JSA1; Q5VXL3; Q96PF9; Q96PG0; E9PB27
• Molecular Weight: 39,175 Da
• NCBI Official Full Name: pre-B-cell leukemia transcription factor 3 isoform 1
• NCBI Official Synonym Full Names: pre-B-cell leukemia homeobox 3
• NCBI Official Symbol: PBX3
• NCBI Protein Information: pre-B-cell leukemia transcription factor 3; homeobox protein PBX3
• UniProt Protein Name: Pre-B-cell leukemia transcription factor 3
• Protein Family: Pre-B-cell leukemia transcription factor
• UniProt Gene Name: PBX3
• UniProt Entry Name: PBX3_HUMAN

Uniprot Description

PBX3: Transcriptional activator that binds the sequence 5'- ATCAATCAA-3'. Belongs to the TALE/PBX homeobox family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: DNA-binding

Chromosomal Location of Human Ortholog: 9q33.3

Cellular Component: transcription factor complex; nucleus

Molecular Function: sequence-specific DNA binding; transcription factor activity

Biological Process: dorsal spinal cord development; posterior compartment specification; anterior compartment specification; transcription, DNA-dependent; regulation of transcription, DNA-dependent; adult locomotory behavior; neuron development; neurological control of breathing; respiratory gaseous exchange