Western Blot (WB)
(Western Blot analysis in T47D cell line lysates (35ug/lane) usingMBS6010323. KCTD12 (arrow) was detected using the purified Pab.)
Rabbit anti-Human KCTD12 Polyclonal Antibody | anti-KCTD12 antibody
KCTD12, ID (KCTD12, C13orf2, KIAA1778, PFET1, BTB/POZ domain-containing protein KCTD12, Pfetin, Predominantly fetal expressed T1 domain) (MaxLight 490)
Gene Names
KCTD12; PFET1; PFETIN; C13orf2
Reactivity
Human
Applications
Flow Cytometry, Functional Assay, Immunohistochemistry, Western Blot
Purity
Purified by Protein A and Peptide Affinity Chromatography.
Synonyms
KCTD12; Polyclonal Antibody; ID (KCTD12; C13orf2; KIAA1778; PFET1; BTB/POZ domain-containing protein KCTD12; Pfetin; Predominantly fetal expressed T1 domain) (MaxLight 490); Predominantly fetal expressed T1 domain; anti-KCTD12 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes human KCTD12.
Purity/Purification
Purified by Protein A and Peptide Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight490.
Applicable Applications for anti-KCTD12 antibody
Flow Cytometry (FC/FACS), FLISA, Immunohistochemistry (IHC), Western Blot (WB)
Application Notes
FC/FACS: 1:10-50
FLISA: 1:1,000
IHC: 1:50-100
WB: 1:100-500
Applications are based on unconjugated antibody.
FLISA: 1:1,000
IHC: 1:50-100
WB: 1:100-500
Applications are based on unconjugated antibody.
Immunogen
KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 242-269 in the central region of human KCTD12, UniProt Accession #Q96CX2;Q6WVG3. Species sequence homology: mouse
Conjugate
MaxLight490
Note
Preservative Free
Preparation and Storage
Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight490 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Western Blot (WB)
(Western Blot analysis in T47D cell line lysates (35ug/lane) usingMBS6010323. KCTD12 (arrow) was detected using the purified Pab.)
Western Blot (WB)
(Western Blot analysis in T47D cell line lysates (35ug/lane) usingMBS6010323. KCTD12 (arrow) was detected using the purified Pab.)
Immunohistochemistry (IHC)
(Immunohistochemistry analysis in formalin-fixed and paraffin-embedded human brain tissue reacted withMBS6010323, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of MBS6010323 for immunohistochemistry.)
Immunohistochemistry (IHC)
(Immunohistochemistry analysis in formalin-fixed and paraffin-embedded human brain tissue reacted withMBS6010323, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of MBS6010323 for immunohistochemistry.)
Flow Cytometry (FC/FACS)
(Flow Cytometry analysis of T47D cells using MBS6010323 (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Flow Cytometry (FC/FACS)
(Flow Cytometry analysis of T47D cells using MBS6010323 (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Related Product Information for anti-KCTD12 antibody
MaxLight490 is a new Blue-Green photostable dye conjugate comparable to DyLight488, Alexa Fluor488 and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (491nm); Emission (515nm); Extinction Coefficient 73,000.
Product Categories/Family for anti-KCTD12 antibody
References
1. Drenos, F., et.al., Hum. Mol. Genet. 18 (12), 2305-2316 (2009). 2. Suehara, Y., et.al., Clin. Cancer Res. 14 (6), 1707-1717 (2008). 3. Cauchi, S., et.al., J. Mol. Med. 86 (3), 341-348 (2008).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
35,701 Da
NCBI Official Full Name
BTB/POZ domain-containing protein KCTD12
NCBI Official Synonym Full Names
potassium channel tetramerization domain containing 12
NCBI Official Symbol
KCTD12
NCBI Official Synonym Symbols
PFET1; PFETIN; C13orf2
NCBI Protein Information
BTB/POZ domain-containing protein KCTD12; predominantly fetal expressed T1 domain; potassium channel tetramerisation domain containing 12
UniProt Protein Name
BTB/POZ domain-containing protein KCTD12
Protein Family
UniProt Gene Name
KCTD12
UniProt Synonym Gene Names
C13orf2; KIAA1778; PFET1
UniProt Entry Name
KCD12_HUMAN
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Product Notes
The KCTD12 kctd12 (Catalog #AAA6312836) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The KCTD12, ID (KCTD12, C13orf2, KIAA1778, PFET1, BTB/POZ domain-containing protein KCTD12, Pfetin, Predominantly fetal expressed T1 domain) (MaxLight 490) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's KCTD12 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), FLISA, Immunohistochemistry (IHC), Western Blot (WB). FC/FACS: 1:10-50 FLISA: 1:1,000 IHC: 1:50-100 WB: 1:100-500 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the KCTD12 kctd12 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "KCTD12, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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