SDS-Page
(Recombinant PARP1 protein gel 8% SDS-PAGE gel stained with Coomassie blue. MW: 115.1 kDa Purity: ?80%)
PARP1 recombinant protein
Recombinant PARP1 protein
Gene Names
METTL1; TRM8; TRMT8; C12orf1; YDL201w
Synonyms
PARP1; Recombinant PARP1 protein; Poly(ADP-Ribose) Polymerase 1; PARP1 recombinant protein
Host
Baculovirus
Form/Format
Recombinant PARP1 protein is supplied at a concentration of 0.6ug/ul in 25mM HEPES pH7.5, 300mM NaCl, 10% glycerol, 0.04% Triton X-100, 0.5mM TCEP.
Protein Species
Human
Tag
DYKDDDDK-Tag
Notes
This product was manufactured as described in Protein Details. Where possible, We has developed functional or activity assays for recombinant proteins. Additional characterization such as enzyme kinetic activity assays, inhibitor screening or other biological activity assays may not have been performed for every product. All available data for a given product is shown on the lot-specific Technical Data Sheet.
Dry Ice Shipment
Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
Preparation and Storage
Recombinant proteins in solution are temperature sensitive and must be stored at -80 degree C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
Shipping Temp: Dry Ice
Shipping Temp: Dry Ice
SDS-Page
(Recombinant PARP1 protein gel 8% SDS-PAGE gel stained with Coomassie blue. MW: 115.1 kDa Purity: ?80%)
SDS-Page
(Recombinant PARP1 protein gel 8% SDS-PAGE gel stained with Coomassie blue. MW: 115.1 kDa Purity: ?80%)
Related Product Information for PARP1 recombinant protein
Short Description: Recombinant PARP1 protein was expressed in a baculovirus expression system as the full-length protein (accession number NP_005362.3) with an N-terminal DYKDDDDK tag. The molecular weight of PARP1 is 115.1 kDa. It is suitable for use in assays that modify histones and CTCF by poly ADP-ribosylation.
Background: PARP1 (Poly(ADP-Ribose) Polymerase 1, also known as PARP; PPOL; ADPRT; ARTD1; ADPRT1; PARP-1; ADPRT 1; pADPRT-1) is a chromatin-associated enzyme, poly(ADP-ribosyl)transferase, which modifies various nuclear proteins by poly(ADP-ribosyl)ation. Poly(ADP-ribosyl)ation is dependent on DNA and is involved in the regulation of various important cellular processes such as differentiation, proliferation, and tumor transformation and also in the regulation of the molecular events involved in the recovery of cell from DNA damage.
Background: PARP1 (Poly(ADP-Ribose) Polymerase 1, also known as PARP; PPOL; ADPRT; ARTD1; ADPRT1; PARP-1; ADPRT 1; pADPRT-1) is a chromatin-associated enzyme, poly(ADP-ribosyl)transferase, which modifies various nuclear proteins by poly(ADP-ribosyl)ation. Poly(ADP-ribosyl)ation is dependent on DNA and is involved in the regulation of various important cellular processes such as differentiation, proliferation, and tumor transformation and also in the regulation of the molecular events involved in the recovery of cell from DNA damage.
Product Categories/Family for PARP1 recombinant protein
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
17,423 Da
NCBI Official Full Name
tRNA (guanine-N(7)-)-methyltransferase isoform a
NCBI Official Synonym Full Names
methyltransferase like 1
NCBI Official Symbol
METTL1
NCBI Official Synonym Symbols
TRM8; TRMT8; C12orf1; YDL201w
NCBI Protein Information
tRNA (guanine-N(7)-)-methyltransferase; tRNA (guanine-N(7)-)-methyltransferase; D1075-like gene product; METTL1; methyltransferase-like 1; methyltransferase-like protein 1; tRNA (guanine(46)-N(7))-methyltransferase; tRNA(m7G46)-methyltransferase
UniProt Protein Name
tRNA (guanine-N(7)-)-methyltransferase
Protein Family
UniProt Gene Name
METTL1
UniProt Entry Name
TRMB_HUMAN
NCBI Description
This gene is similar in sequence to the S. cerevisiae YDL201w gene. The gene product contains a conserved S-adenosylmethionine-binding motif and is inactivated by phosphorylation. Alternative splice variants encoding different protein isoforms have been described for this gene. A pseudogene has been identified on chromosome X. [provided by RefSeq, Jul 2008]
Uniprot Description
METTL1: Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA. Belongs to the methyltransferase superfamily. TrmB family.
Protein type: Methyltransferase; EC 2.1.1.33; RNA processing
Chromosomal Location of Human Ortholog: 12q13
Cellular Component: nucleoplasm; cytoplasm; nucleolus; nucleus
Molecular Function: protein binding; tRNA (guanine-N7-)-methyltransferase activity; tRNA binding
Biological Process: tRNA modification; tRNA methylation
Research Articles on PARP1
Similar Products
Product Notes
The PARP1 mettl1 (Catalog #AAA389096) is a Recombinant Protein produced from Baculovirus and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "PARP1, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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