Rabbit anti-Human KCNK7 Polyclonal Antibody | anti-KCNK7 antibody

KCNK7 Antibody (C-term) Affinity Purified Rabbit Polyclonal Antibody (Pab)

Cat. Num. AAA9232380

• Gene Names: KCNK7; TWIK3; K2p7.1
• Reactivity: Human
• Applications: Western Blot, Immunohistochemistry, Flow Cytometry, Functional Assay, ELISA
• Purity: This antibody is purified through a protein A column, followed by peptide affinity purification.
• Synonyms: KCNK7; Polyclonal Antibody; KCNK7 Antibody (C-term) Affinity Purified Rabbit Polyclonal Antibody (Pab); Potassium channel subfamily K member 7; anti-KCNK7 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Clone Number: RB27360
• Specificity: This KCNK7 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 259-288 amino acids from the C-terminal region of human KCNK7.
• Purity/Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.

• Applicable Applications for anti-KCNK7 antibody: Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Flow Cytometry (FC/FACS), ELISA (EIA)
• Application Notes: WB: 1:1000; IHC-P: 1:50-100; FC/FACS: 1:10-50
• Antigen Source: Human
• Antigen Type: Synthetic Peptide
• Antigen Region: 259-288
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 2 weeks. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(WB: 1:1000KCNK7 Antibody (C-term) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the KCNK7 antibody detected the KCNK7 protein (arrow).)

Immunohistochemistry (IHC)-Paraffin

(IHC-P: 1:50-100KCNK7 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the KCNK7 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(FC: 1:10~50KCNK7 Antibody (C-term) flow cytometric analysis of A549 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-KCNK7 antibody

KCNK7 is a member of the superfamily of potassiumchannel proteins containing two pore-forming P domains. The productof this gene has not been shown to be a functional channel;however, it may require other non-pore-forming proteins foractivity. Multiple transcript variants encoding different isoformshave been found for this gene.

Product Categories/Family for anti-KCNK7 antibody

Primary Antibodies; Neuroscience

References

Goldstein, S.A., et al. Pharmacol. Rev. 57(4):527-540(2005)Goldstein, S.A., et al. Nat. Rev. Neurosci. 2(3):175-184(2001)Medhurst, A.D., et al. Brain Res. Mol. Brain Res. 86 (1-2), 101-114 (2001):Salinas, M., et al. J. Biol. Chem. 274(17):11751-11760(1999)

NCBI and Uniprot Product Information

• NCBI GI #: 5031821
• NCBI GeneID: 10089
• NCBI Accession #: NP_005705.1; NP_203133.1; NP_203134.1; NP_258416.1
• NCBI GenBank Nucleotide #: NM_005714.1
• UniProt Accession #: Q9Y2U2; Q3SYI2; Q9Y2U3; Q9Y2U4
• Molecular Weight: 31,947 Da
• NCBI Official Full Name: potassium channel subfamily K member 7 isoform C
• NCBI Official Synonym Full Names: potassium channel, subfamily K, member 7
• NCBI Official Symbol: KCNK7
• NCBI Official Synonym Symbols: TWIK3; K2p7.1
• NCBI Protein Information: potassium channel subfamily K member 7; two pore domain K+ channel; potassium channel, subfamily K, member 7, isoform B
• UniProt Protein Name: Potassium channel subfamily K member 7
• Protein Family: Potassium channel subfamily
• UniProt Gene Name: KCNK7
• UniProt Entry Name: KCNK7_HUMAN

NCBI Description

This gene encodes a member of the superfamily of potassium channel proteins containing two pore-forming P domains. The product of this gene has not been shown to be a functional channel; however, it may require other non-pore-forming proteins for activity. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

KCNK7: Probable potassium channel subunit. No channel activity observed in vitro as protein remains in the endoplasmic reticulum. May need to associate with an as yet unknown partner in order to reach the plasma membrane. Belongs to the two pore domain potassium channel (TC 1.A.1.8) family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, multi-pass; Membrane protein, integral

Chromosomal Location of Human Ortholog: 11q13

Cellular Component: integral to membrane; plasma membrane

Molecular Function: potassium channel activity; voltage-gated ion channel activity

Biological Process: synaptic transmission; potassium ion transport