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Western Blot (WB) (DNAJB2 Antibody (N-term) western blot analysis in MDA-MB453 cell line lysates (35ug/lane).This demonstrates the DNAJB2 antibody detected the DNAJB2 protein (arrow).)

Rabbit anti-Human DNAJB2 Polyclonal Antibody | anti-DNAJB2 antibody

DNAJB2, NT (DNAJB2, HSJ1, HSPF3, DnaJ homolog subfamily B member 2, DnaJ protein homolog 1, Heat shock 40kD protein 3, Heat shock protein J1) (APC)

Gene Names
DNAJB2; HSJ1; CMT2T; DSMA5; HSJ-1; HSPF3
Reactivity
Human
Applications
Western Blot
Purity
Purified by Protein A Affinity Chromatography.
Synonyms
DNAJB2; Polyclonal Antibody; NT (DNAJB2; HSJ1; HSPF3; DnaJ homolog subfamily B member 2; DnaJ protein homolog 1; Heat shock 40kD protein 3; Heat shock protein J1) (APC); Heat shock protein J1; anti-DNAJB2 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Purified by Protein A Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).
Sequence Length
324
Applicable Applications for anti-DNAJB2 antibody
Western Blot (WB), FLISA
Application Notes
WB: 1:100-500
FLISA: 1:1,000
Applications are based on unconjugated antibody.
Immunogen
DNAJB2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 42-71 amino acids from the N-terminal region of human DNAJB2.
Conjugate
APC
Note
Preservative Free
Special Handling
Light sensitive
Preparation and Storage
Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(DNAJB2 Antibody (N-term) western blot analysis in MDA-MB453 cell line lysates (35ug/lane).This demonstrates the DNAJB2 antibody detected the DNAJB2 protein (arrow).)

Western Blot (WB) (DNAJB2 Antibody (N-term) western blot analysis in MDA-MB453 cell line lysates (35ug/lane).This demonstrates the DNAJB2 antibody detected the DNAJB2 protein (arrow).)
Related Product Information for anti-DNAJB2 antibody
DNAJB2 is involved in heat shock protein and unfolded protein binding.
Product Categories/Family for anti-DNAJB2 antibody
References
Claeys, K.G., et al. Am. J. Pathol. 176(6):2901-2910(2010). Starr, J.M., et al. Mech. Ageing Dev. 129(12):745-751(2008). Mahoney, D.J., et al. Am. J. Physiol. Regul. Integr. Comp. Physiol. 294 (6), R1901-R1910 (2008): Adaimy, L., et al. Am. J. Hum. Genet. 81(4):821-828(2007). Sugiyama, N., et al. Mol. Cell Proteomics 6(6):1103-1109(2007).

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
dnaJ homolog subfamily B member 2 isoform b
NCBI Official Synonym Full Names
DnaJ heat shock protein family (Hsp40) member B2
NCBI Official Symbol
DNAJB2
NCBI Official Synonym Symbols
HSJ1; CMT2T; DSMA5; HSJ-1; HSPF3
NCBI Protein Information
dnaJ homolog subfamily B member 2
UniProt Protein Name
DnaJ homolog subfamily B member 2
Protein Family
UniProt Gene Name
DNAJB2
UniProt Synonym Gene Names
HSJ1; HSPF3; HSJ-1
UniProt Entry Name
DNJB2_HUMAN

NCBI Description

This gene is almost exclusively expressed in the brain, mainly in the neuronal layers. It encodes a protein that shows sequence similarity to bacterial DnaJ protein and the yeast homologs. In bacteria, this protein is implicated in protein folding and protein complex dissociation. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Jul 2011]

Uniprot Description

DNAJB2: 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Chaperone

Chromosomal Location of Human Ortholog: 2q35

Cellular Component: proteasome complex; inclusion body; nucleus; cytosol

Molecular Function: protein binding; chaperone binding; unfolded protein binding; Hsp70 protein binding; polyubiquitin binding

Biological Process: negative regulation of cell proliferation; ER-associated protein catabolic process; protein folding; positive regulation of protein ubiquitination; positive regulation of proteasomal ubiquitin-dependent protein catabolic process; protein refolding; negative regulation of cell growth; response to unfolded protein

Disease: Spinal Muscular Atrophy, Distal, Autosomal Recessive, 5; Charcot-marie-tooth Disease, Axonal, Type 2t

Research Articles on DNAJB2

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Product Notes

The DNAJB2 dnajb2 (Catalog #AAA6292675) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The DNAJB2, NT (DNAJB2, HSJ1, HSPF3, DnaJ homolog subfamily B member 2, DnaJ protein homolog 1, Heat shock 40kD protein 3, Heat shock protein J1) (APC) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's DNAJB2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), FLISA. WB: 1:100-500 FLISA: 1:1,000 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the DNAJB2 dnajb2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "DNAJB2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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