Rabbit anti-Human, Mouse KappaB ras1 Polyclonal Antibody | anti-NKIRAS1 antibody

KappaB ras1 Antibody

Cat. Num. AAA150526

• Gene Names: NKIRAS1; KBRAS1; kappaB-Ras1
• Reactivity: Human, Mouse
• Applications: ELISA, Western Blot, Immunocytochemistry
• Purity: KappaB ras1 Antibody is affinity chromatography purified via peptide column.
• Synonyms: KappaB ras1; Polyclonal Antibody; KappaB ras1 Antibody; KBRAS1; kappaB-Ras1; NF-kappa-B inhibitor-interacting Ras-like protein 1; I-kappa-B-interacting Ras-like protein 1; Kappa B-Ras protein 1; NFKB inhibitor interacting Ras-like 1; anti-NKIRAS1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: KappaB ras1 Antibody is affinity chromatography purified via peptide column.
• Form/Format: Liquid
• Sequence Length: 192

• Applicable Applications for anti-NKIRAS1 antibody: ELISA (EIA), Western Blot (WB), Immunocytochemistry (ICC)
• Application Notes: KappaB ras1 antibody can be used for detection of KappaB ras1 by Western blot at 0.5 - 2 mug/mL. Antibody can also be used for immunocytochemistry starting at 1 mug/mL.
• Conjugate: Unconjugated
• Immunogen: KappaB ras1 antibody was raised against a 15 amino acid synthetic peptide from near the carboxy terminus of human KappaB ras1.
• Buffer: KappaB ras1 Antibody is supplied in PBS containing 0.02% sodium azide.
• Preparation and Storage: KappaB ras1 antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Western Blot (WB)

(Western blot analysis of KappaB ras1 in RAW264.7 cell lysate with KappaB ras1 antibody at (A) 0.5, (B) 1 and (C) 2 μg/mL.)

Immunocytochemistry (IHC)

(Immunocytochemistry of KappaB-ras1 in RAW264.7 cells with KappaB-ras1 antibody at 1 μg/mL.)

Related Product Information for anti-NKIRAS1 antibody

KappaB ras1 Antibody: KappaB ras-1 (κB-ras-1) and kappaB-ras-2 are two small proteins that similar to Ras-like small GTPases that associate with IκB (IκB), an inhibitor of the transcription factor NF-κB. IκB exists in two homologous forms, IκB-alpha and IκB-beta, although IκB-beta contains a unique 47-amino acid region within its ankyrin domain. While inactive IκB-alpha-NF-κB complexes can shuttle in and out of the nucleus, IκB-beta-NF-κB complexes are retained exclusively in the cytoplasm. It is suggested that kappaB-ras proteins preferentially bind to the IκB-beta form through this unique insert within the ankyrin region, thus modulating the cellular location of IκB-beta and regulating the rate of degradation of IκB-beta. This antibody is specific for kappaB-ras1 and has no cross-reactivity to kappaB-ras2.

NCBI and Uniprot Product Information

• NCBI GI #: 7008402
• NCBI GeneID: 28512
• UniProt Accession #: Q9NYS0; Q96K18
• Molecular Weight: 21,643 Da
• NCBI Official Full Name: kappa B-ras 1
• NCBI Official Synonym Full Names: NFKB inhibitor interacting Ras-like 1
• NCBI Official Symbol: NKIRAS1
• NCBI Official Synonym Symbols: KBRAS1; kappaB-Ras1
• NCBI Protein Information: NF-kappa-B inhibitor-interacting Ras-like protein 1; kappa B-ras 1; kappa B-Ras protein 1; I-kappa-B-interacting Ras-like protein 1; NFKB inhibitor interacting Ras-like protein 1
• UniProt Protein Name: NF-kappa-B inhibitor-interacting Ras-like protein 1
• Protein Family: NF-kappa-B inhibitor-interacting Ras-like protein
• UniProt Gene Name: NKIRAS1
• UniProt Synonym Gene Names: KBRAS1; Kappa B-Ras protein 1; KappaB-Ras1
• UniProt Entry Name: KBRS1_HUMAN

Uniprot Description

NKIRAS1: Atypical Ras-like protein that acts as a potent regulator of NF-kappa-B activity by preventing the degradation of NF-kappa-B inhibitor beta (NFKBIB) by most signals, explaining why NFKBIB is more resistant to degradation. May act by blocking phosphorylation of NFKBIB and mediating cytoplasmic retention of p65/RELA NF-kappa-B subunit. It is unclear whether it acts as a GTPase. Both GTP- and GDP-bound forms block phosphorylation of NFKBIB. Belongs to the small GTPase superfamily. Ras family. KappaB-Ras subfamily.

Protein type: G protein; G protein, monomeric, Ras; G protein, monomeric

Chromosomal Location of Human Ortholog: 3p24.2

Cellular Component: membrane; cytoplasm

Molecular Function: GTPase activity; GTP binding

Biological Process: I-kappaB kinase/NF-kappaB cascade; metabolic process; small GTPase mediated signal transduction