Western Blot (WB)
(Western blot analysis of K22E using MCF7 whole cell lysates)
Rabbit anti-Human K22E Polyclonal Antibody | anti-K22E antibody
K22E Antibody
Gene Names
KRT2; K2e; KRTE; CK-2e; KRT2A; KRT2E
Reactivity
Human
Applications
Western Blot, ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
K22E; Polyclonal Antibody; K22E Antibody; CK 2e; Cytokeratin-2e; Epithelial keratin-2e; K2e; Keratin type II cytoskeletal 2 epidermal; Keratin-2 epidermis; KRT2; KRT2A; KRT2E; Type-II keratin Kb2; anti-K22E antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
K22E antibody detects endogenous levels of total K22E
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
639
Applicable Applications for anti-K22E antibody
Western Blot (WB), ELISA (EIA)
Application Notes
WB: 1:1000-3000
Immunogen
A synthesized peptide derived from human K22E
Subcellular Location
Cytoplasmic
Tissue Specificity
Expressed in the upper spinous and granular suprabasal layers of normal adult epidermal tissues from most body sites including thigh, breast nipple, foot sole, penile shaft and axilla. Not present in foreskin, squamous metaplasias and carcinomas. Expression in hypertrophic and keloid scars begins in the deepest suprabasal layer. Weakly expressed in normal gingiva and tongue, however expression is induced in benign keratoses of lingual mucosa and in mild-to-moderate oral dysplasia with orthokeratinization.
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Western Blot (WB)
(Western blot analysis of K22E using MCF7 whole cell lysates)
Western Blot (WB)
(Western blot analysis of K22E using MCF7 whole cell lysates)
Western Blot (WB)
(Western blot analysis of K22E using MCF7 whole cell lysates)
Western Blot (WB)
(Western blot analysis of K22E using MCF7 whole cell lysates)
Related Product Information for anti-K22E antibody
Function: Probably contributes to terminal cornification (PubMed:1380918). Associated with keratinocyte activation, proliferation and keratinization (PubMed:12598329). Plays a role in the establishment of the epidermal barrier on plantar skin (By similarity).
Subunit Structure: Heterotetramer of two type I and two type II keratins. Associates with KRT10 (By similarity).
Similarity: Belongs to the intermediate filament family.
Subunit Structure: Heterotetramer of two type I and two type II keratins. Associates with KRT10 (By similarity).
Similarity: Belongs to the intermediate filament family.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Observed: 65 kDa
Predicted: 66 kDa
Predicted: 66 kDa
NCBI Official Full Name
keratin, type II cytoskeletal 2 epidermal
NCBI Official Synonym Full Names
keratin 2
NCBI Official Symbol
KRT2
NCBI Official Synonym Symbols
K2e; KRTE; CK-2e; KRT2A; KRT2E
NCBI Protein Information
keratin, type II cytoskeletal 2 epidermal
UniProt Protein Name
Keratin, type II cytoskeletal 2 epidermal
UniProt Gene Name
KRT2
UniProt Synonym Gene Names
KRT2A; KRT2E; CK-2e; K2e
NCBI Description
The protein encoded by this gene is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. This type II cytokeratin is expressed largely in the upper spinous layer of epidermal keratinocytes and mutations in this gene have been associated with bullous congenital ichthyosiform erythroderma. The type II cytokeratins are clustered in a region of chromosome 12q12-q13. [provided by RefSeq, Jul 2008]
Uniprot Description
Probably contributes to terminal cornification (PubMed:1380918). Associated with keratinocyte activation, proliferation and keratinization (PubMed:12598329). Plays a role in the establishment of the epidermal barrier on plantar skin ().
Research Articles on K22E
Similar Products
Product Notes
The K22E krt2 (Catalog #AAA9609325) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The K22E Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's K22E can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB: 1:1000-3000. Researchers should empirically determine the suitability of the K22E krt2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "K22E, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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