Rabbit JunB Polyclonal Antibody | anti-JunB antibody

JunB (Phospho-Ser259)

Cat. Num. AAA191039

• Gene Names: JUNB; AP-1
• Applications: Immunoblot, Western Blot, Immunohistochemistry
• Synonyms: JunB; Polyclonal Antibody; JunB (Phospho-Ser259); anti-JunB antibody

• Host: Rabbit
• Clonality: Polyclonal
• Specificity: This antibody detects endogenous human, mouse, and rat JunB only when phosphorylated at serine 259.
• Form/Format: 100ul at 1.0mg/ml in PBS (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Affinity-purified on phosphopeptide; non-phosphopeptide- reactive antibodies were removed by chromatography on non-phosphorylated peptide.
• Sequence Length: 347

• Applicable Applications for anti-JunB antibody: Immunoblotting (IB), Immunohistochemistry (IHC)
• Application Notes: Immunoblotting: use at dilution of 1:500-1:1,000. A band of ~43kDa is detected.; Immunohistochemistry: use at dilution of 1:50- 1:100.; Dilute in PBS or medium that is identical to that used in the assay system.
• Antigen: Peptide sequence that includes phosphorylation site of serine 259 (P-B-S(p)-P-I) derived from human JunB and conjugated to KLH.
• Preparation and Storage: This antibody is stable for at least one (1) year at -20 degree C. Can be stored at 4 degree C for short-term use.

Immunohistochemistry (IHC)

Testing Data

Related Product Information for anti-JunB antibody

Transcription factor JunB is a protein that in humans is encoded by the JUNB gene and is involved in regulating gene activity following the primary growth factor response. It binds to the DNA sequence 5'-TGA[CG]TCA-3'.

NCBI and Uniprot Product Information

• NCBI GI #: 4504809
• NCBI GeneID: 3726
• NCBI Accession #: NP_002220.1
• NCBI GenBank Nucleotide #: NM_002229.2
• UniProt Accession #: P17275; Q96GH3
• Molecular Weight: 35,879 Da
• NCBI Official Full Name: transcription factor jun-B
• NCBI Official Synonym Full Names: JunB proto-oncogene, AP-1 transcription factor subunit
• NCBI Official Symbol: JUNB
• NCBI Official Synonym Symbols: AP-1
• NCBI Protein Information: transcription factor jun-B
• UniProt Protein Name: Transcription factor jun-B
• Protein Family: Transcription factor
• UniProt Gene Name: JUNB

Uniprot Description

JunB: Transcription factor involved in regulating gene activity following the primary growth factor response. Binds to the DNA sequence 5'-TGA[CG]TCA-3'. Binds DNA as an homodimer or as an heterodimer with another member of the Jun/Fos family. Interacts with ITCH (via its WW domains). By growth factors. Belongs to the bZIP family. Jun subfamily.

Protein type: Motility/polarity/chemotaxis; Oncoprotein; Transcription factor

Chromosomal Location of Human Ortholog: 19p13.13

Cellular Component: chromatin; nuclear chromatin; nucleoplasm; transcription factor complex

Molecular Function: DNA binding; protein binding; transcription coactivator activity; transcription corepressor activity; transcription factor binding

Biological Process: cellular response to hormone stimulus; negative regulation of transcription from RNA polymerase II promoter; positive regulation of cell differentiation; positive regulation of transcription from RNA polymerase II promoter; regulation of cell cycle; regulation of cell proliferation; regulation of transcription from RNA polymerase II promoter; response to cAMP; response to cytokine stimulus; response to drug; response to lipopolysaccharide; response to mechanical stimulus; response to radiation