IL-21 active protein

Murine IL-21

Cat. Num. AAA695720

• Gene Names: Il21; IL-21
• Reactivity: Species Reactivity: Mouse
• Purity: Above 98% as determined by SDS-PAGE Analysis.
• Synonyms: IL-21; Murine IL-21; Recombinant Murine IL-21; IL-21 active protein

• Host: E Coli
• Reactivity: Species Reactivity: Mouse
• Purity/Purification: Above 98% as determined by SDS-PAGE Analysis.
• Sequence: MHKSSPQGP DRLLIRLRH LIDIVEQLK IYENDLDPE LLSAPQDVK GHCEHAAFA CFQKAKLKP SNPGNNKTF IIDLVAQLR RRLPARRGG KKQKHIAKC PSCDSYEKR TPKEFLERL KWLLQKMIH QHLS

• Biological Activity: Measured by its ability to increase proliferation in mouse splenocytes induced by anti-hCD40 mAb. The expected ED50 is 15-20 ng/ml.
• Cell Type: B Cells, T Cells
• Preparation and Storage: At -20 degree C

Testing Data

Related Product Information for IL-21 active protein

IL-21 is a pleiotropic cytokine produced by CD4+ T cells in response to antigenic stimulation. Its action generally enhances antigen-specific responses of immune cells. The biological effects of IL-21 include induction of differentiation of T-cells-stimulated-B-cells into plasma cells and memory B-cells, stimulation (in conjunction) with IL-4 of IgG production, and induction of apoptotic effects in naive B-cells and stimulated B-cells in the absence of T-cell signaling. Additionally, IL-21 promotes the anti-tumor activity of CD8+ T-cells and NK cells. IL-21 exerts its effect through binding to a specific type I cytokine receptor, IL-21R, which also contains the gamma chain (gammac) found in other cytokine receptors including IL-2, IL-4, IL-7, IL-9 and IL-15. The IL-21/IL-21R interaction triggers a cascade of events which includes activation of the tyrosine kinases JAK1 and JAK3, followed by activation of the transcription factors STAT1 and STAT3. Recombinant murine IL-21 is a 15.0 kDa protein consisting of 130 amino acid residues.

Product Categories/Family for IL-21 active protein

Adaptive Immunity; Stem Cell Research; Apoptosis; Immunology; Cancer

NCBI and Uniprot Product Information

• NCBI GI #: 55976590
• NCBI GeneID: 60505
• UniProt Accession #: Q9ES17
• Molecular Weight: 16,811 Da
• NCBI Official Full Name: Interleukin-21
• NCBI Official Synonym Full Names: interleukin 21
• NCBI Official Symbol: Il21
• NCBI Official Synonym Symbols: IL-21
• NCBI Protein Information: interleukin-21
• UniProt Protein Name: Interleukin-21
• UniProt Gene Name: Il21
• UniProt Synonym Gene Names: IL-21
• UniProt Entry Name: IL21_MOUSE

Uniprot Description

IL21: Cytokine with immunoregulatory activity. May promote the transition between innate and adaptive immunity. Induces the production of IgG(1) and IgG(3) in B-cells. May play a role in proliferation and maturation of natural killer (NK) cells in synergy with IL15. May regulate proliferation of mature B- and T-cells in response to activating stimuli. In synergy with IL15 and IL18 stimulates interferon gamma production in T-cells and NK cells. During T-cell mediated immune response may inhibit dendritic cells (DC) activation and maturation. Belongs to the IL-15/IL-21 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Apoptosis; Secreted; Cell development/differentiation; Cytokine; Secreted, signal peptide

Cellular Component: extracellular space; extracellular region

Molecular Function: hematopoietin/interferon-class (D200-domain) cytokine receptor binding; interleukin-2 receptor binding; cytokine activity

Biological Process: positive regulation of interleukin-17 production; positive regulation of natural killer cell cytokine production; cell maturation; B cell apoptosis; positive regulation of natural killer cell mediated cytotoxicity; positive regulation of interleukin-10 production; positive regulation of tyrosine phosphorylation of Stat3 protein; positive regulation of natural killer cell differentiation; positive regulation of tyrosine phosphorylation of Stat1 protein; positive regulation of cell proliferation; positive regulation of B cell proliferation; positive regulation of B cell differentiation; positive regulation of T cell proliferation; immune response; positive regulation of interferon-gamma biosynthetic process